ACPX_STRCO
ID ACPX_STRCO Reviewed; 86 AA.
AC Q02054;
DT 01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1995, sequence version 1.
DT 25-MAY-2022, entry version 142.
DE RecName: Full=Actinorhodin polyketide synthase acyl carrier protein;
DE Short=ACP;
DE AltName: Full=actI ORF3;
GN OrderedLocusNames=SCO5089; ORFNames=SCBAC28G1.15;
OS Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145).
OC Bacteria; Actinobacteria; Streptomycetales; Streptomycetaceae;
OC Streptomyces; Streptomyces albidoflavus group.
OX NCBI_TaxID=100226;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PHOSPHOPANTETHEINYLATION AT SER-42.
RC STRAIN=ATCC BAA-471 / A3(2) / M145;
RX PubMed=1527048; DOI=10.1016/s0021-9258(18)41772-3;
RA Fernandez-Moreno M.A., Martinez E., Boto L., Hopwood D.A., Malpartida F.;
RT "Nucleotide sequence and deduced functions of a set of cotranscribed genes
RT of Streptomyces coelicolor A3(2) including the polyketide synthase for the
RT antibiotic actinorhodin.";
RL J. Biol. Chem. 267:19278-19290(1992).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC BAA-471 / A3(2) / M145;
RX PubMed=12000953; DOI=10.1038/417141a;
RA Bentley S.D., Chater K.F., Cerdeno-Tarraga A.-M., Challis G.L.,
RA Thomson N.R., James K.D., Harris D.E., Quail M.A., Kieser H., Harper D.,
RA Bateman A., Brown S., Chandra G., Chen C.W., Collins M., Cronin A.,
RA Fraser A., Goble A., Hidalgo J., Hornsby T., Howarth S., Huang C.-H.,
RA Kieser T., Larke L., Murphy L.D., Oliver K., O'Neil S., Rabbinowitsch E.,
RA Rajandream M.A., Rutherford K.M., Rutter S., Seeger K., Saunders D.,
RA Sharp S., Squares R., Squares S., Taylor K., Warren T., Wietzorrek A.,
RA Woodward J.R., Barrell B.G., Parkhill J., Hopwood D.A.;
RT "Complete genome sequence of the model actinomycete Streptomyces coelicolor
RT A3(2).";
RL Nature 417:141-147(2002).
RN [3]
RP STRUCTURE BY NMR.
RC STRAIN=A3(2) / NRRL B-16638;
RX PubMed=9166770; DOI=10.1021/bi970006+;
RA Crump M.P., Crosby J., Dempsey C.E., Parkinson J.A., Murray M.,
RA Hopwood D.A., Simpson T.J.;
RT "Solution structure of the actinorhodin polyketide synthase acyl carrier
RT protein from Streptomyces coelicolor A3(2).";
RL Biochemistry 36:6000-6008(1997).
CC -!- FUNCTION: Acyl carrier protein.
CC -!- PATHWAY: Antibiotic biosynthesis; actinorhodin biosynthesis.
CC -!- PTM: 4'-phosphopantetheine is transferred from CoA to a specific serine
CC of the apo-ACP-like protein. {ECO:0000305}.
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DR EMBL; X63449; CAA45045.1; -; Genomic_DNA.
DR EMBL; AL939122; CAC44202.1; -; Genomic_DNA.
DR PIR; S25842; S25842.
DR RefSeq; NP_629239.1; NC_003888.3.
DR RefSeq; WP_003973889.1; NZ_VNID01000008.1.
DR PDB; 1AF8; NMR; -; A=1-86.
DR PDB; 2AF8; NMR; -; A=1-86.
DR PDB; 2K0X; NMR; -; A=1-86.
DR PDB; 2K0Y; NMR; -; A=1-86.
DR PDB; 2KG6; NMR; -; A=1-86.
DR PDB; 2KG8; NMR; -; A=1-86.
DR PDB; 2KG9; NMR; -; A=1-86.
DR PDB; 2KGA; NMR; -; A=1-86.
DR PDB; 2KGC; NMR; -; A=1-86.
DR PDB; 2KGD; NMR; -; A=1-86.
DR PDB; 2KGE; NMR; -; A=1-86.
DR PDB; 2MVU; NMR; -; A=1-86.
DR PDB; 2MVV; NMR; -; A=1-86.
DR PDBsum; 1AF8; -.
DR PDBsum; 2AF8; -.
DR PDBsum; 2K0X; -.
DR PDBsum; 2K0Y; -.
DR PDBsum; 2KG6; -.
DR PDBsum; 2KG8; -.
DR PDBsum; 2KG9; -.
DR PDBsum; 2KGA; -.
DR PDBsum; 2KGC; -.
DR PDBsum; 2KGD; -.
DR PDBsum; 2KGE; -.
DR PDBsum; 2MVU; -.
DR PDBsum; 2MVV; -.
DR AlphaFoldDB; Q02054; -.
DR BMRB; Q02054; -.
DR SMR; Q02054; -.
DR STRING; 100226.SCO5089; -.
DR DrugBank; DB08585; S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] hexanethioate.
DR DrugBank; DB08586; S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] octanethioate.
DR GeneID; 1100530; -.
DR KEGG; sco:SCO5089; -.
DR PATRIC; fig|100226.15.peg.5169; -.
DR eggNOG; COG0236; Bacteria.
DR HOGENOM; CLU_108696_12_0_11; -.
DR InParanoid; Q02054; -.
DR OMA; EFTELGY; -.
DR PhylomeDB; Q02054; -.
DR UniPathway; UPA00173; -.
DR EvolutionaryTrace; Q02054; -.
DR PRO; PR:Q02054; -.
DR Proteomes; UP000001973; Chromosome.
DR GO; GO:0017000; P:antibiotic biosynthetic process; IEA:UniProtKB-KW.
DR Gene3D; 1.10.1200.10; -; 1.
DR InterPro; IPR036736; ACP-like_sf.
DR InterPro; IPR009081; PP-bd_ACP.
DR InterPro; IPR006162; Ppantetheine_attach_site.
DR Pfam; PF00550; PP-binding; 1.
DR SUPFAM; SSF47336; SSF47336; 1.
DR PROSITE; PS50075; CARRIER; 1.
DR PROSITE; PS00012; PHOSPHOPANTETHEINE; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Antibiotic biosynthesis; Phosphopantetheine; Phosphoprotein;
KW Reference proteome.
FT CHAIN 1..86
FT /note="Actinorhodin polyketide synthase acyl carrier
FT protein"
FT /id="PRO_0000180255"
FT DOMAIN 4..82
FT /note="Carrier"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00258"
FT MOD_RES 42
FT /note="O-(pantetheine 4'-phosphoryl)serine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00258,
FT ECO:0000269|PubMed:1527048, ECO:0000269|PubMed:9166770"
FT HELIX 7..15
FT /evidence="ECO:0007829|PDB:1AF8"
FT STRAND 18..22
FT /evidence="ECO:0007829|PDB:2KG8"
FT TURN 26..28
FT /evidence="ECO:0007829|PDB:1AF8"
FT HELIX 30..32
FT /evidence="ECO:0007829|PDB:2KG8"
FT TURN 35..39
FT /evidence="ECO:0007829|PDB:1AF8"
FT TURN 42..44
FT /evidence="ECO:0007829|PDB:1AF8"
FT HELIX 45..52
FT /evidence="ECO:0007829|PDB:1AF8"
FT TURN 53..56
FT /evidence="ECO:0007829|PDB:1AF8"
FT TURN 62..67
FT /evidence="ECO:0007829|PDB:1AF8"
FT HELIX 71..85
FT /evidence="ECO:0007829|PDB:1AF8"
SQ SEQUENCE 86 AA; 9248 MW; FF9D516AC14E212D CRC64;
MATLLTTDDL RRALVECAGE TDGTDLSGDF LDLRFEDIGY DSLALMETAA RLESRYGVSI
PDDVAGRVDT PRELLDLING ALAEAA
