ID   CSHB_LISMO              Reviewed;         435 AA.
AC   Q8Y755;
DT   03-SEP-2014, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2002, sequence version 1.
DT   03-AUG-2022, entry version 130.
DE   RecName: Full=DEAD-box ATP-dependent RNA helicase CshB {ECO:0000255|HAMAP-Rule:MF_01494};
DE            EC=3.6.4.13 {ECO:0000255|HAMAP-Rule:MF_01494};
GN   Name=cshB {ECO:0000255|HAMAP-Rule:MF_01494}; OrderedLocusNames=lmo1450;
OS   Listeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Listeriaceae; Listeria.
OX   NCBI_TaxID=169963;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-679 / EGD-e;
RX   PubMed=11679669; DOI=10.1126/science.1063447;
RA   Glaser P., Frangeul L., Buchrieser C., Rusniok C., Amend A., Baquero F.,
RA   Berche P., Bloecker H., Brandt P., Chakraborty T., Charbit A.,
RA   Chetouani F., Couve E., de Daruvar A., Dehoux P., Domann E.,
RA   Dominguez-Bernal G., Duchaud E., Durant L., Dussurget O., Entian K.-D.,
RA   Fsihi H., Garcia-del Portillo F., Garrido P., Gautier L., Goebel W.,
RA   Gomez-Lopez N., Hain T., Hauf J., Jackson D., Jones L.-M., Kaerst U.,
RA   Kreft J., Kuhn M., Kunst F., Kurapkat G., Madueno E., Maitournam A.,
RA   Mata Vicente J., Ng E., Nedjari H., Nordsiek G., Novella S., de Pablos B.,
RA   Perez-Diaz J.-C., Purcell R., Remmel B., Rose M., Schlueter T., Simoes N.,
RA   Tierrez A., Vazquez-Boland J.-A., Voss H., Wehland J., Cossart P.;
RT   "Comparative genomics of Listeria species.";
RL   Science 294:849-852(2001).
RN   [2]
RP   FUNCTION, INDUCTION, AND DISRUPTION PHENOTYPE.
RC   STRAIN=ATCC BAA-679 / EGD-e;
RX   PubMed=22820328; DOI=10.1128/aem.01526-12;
RA   Markkula A., Lindstrom M., Johansson P., Bjorkroth J., Korkeala H.;
RT   "Roles of four putative DEAD-box RNA helicase genes in growth of Listeria
RT   monocytogenes EGD-e under heat, pH, osmotic, ethanol, and oxidative stress
RT   conditions.";
RL   Appl. Environ. Microbiol. 78:6875-6882(2012).
RN   [3]
RP   FUNCTION, INDUCTION, AND DISRUPTION PHENOTYPE.
RC   STRAIN=ATCC BAA-679 / EGD-e;
RX   PubMed=22564273; DOI=10.1111/j.1462-2920.2012.02761.x;
RA   Markkula A., Mattila M., Lindstrom M., Korkeala H.;
RT   "Genes encoding putative DEAD-box RNA helicases in Listeria monocytogenes
RT   EGD-e are needed for growth and motility at 3 degrees C.";
RL   Environ. Microbiol. 14:2223-2232(2012).
CC   -!- FUNCTION: DEAD-box RNA helicase involved in cold tolerance, motility,
CC       and tolerance to heat, alkali and oxidative stress.
CC       {ECO:0000269|PubMed:22564273, ECO:0000269|PubMed:22820328}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01494};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01494}.
CC   -!- INDUCTION: By growth at 3 degrees Celsius (PubMed:22564273), and 3.5%
CC       ethanol (PubMed:22820328). {ECO:0000269|PubMed:22564273,
CC       ECO:0000269|PubMed:22820328}.
CC   -!- DISRUPTION PHENOTYPE: Growth rate decreased at 37 and 25 degrees
CC       Celsius, almost completely stopped at 3 degrees Celsius. No motility at
CC       25 or 3 degrees Celsius (PubMed:22564273). Decreased growth at 42
CC       degrees Celsius, pH 9.4 and in the presence of 5 mM H(2)O(2)
CC       (PubMed:22820328). {ECO:0000269|PubMed:22564273,
CC       ECO:0000269|PubMed:22820328}.
CC   -!- SIMILARITY: Belongs to the DEAD box helicase family. CshB subfamily.
CC       {ECO:0000255|HAMAP-Rule:MF_01494}.
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DR   EMBL; AL591979; CAC99528.1; -; Genomic_DNA.
DR   PIR; AB1256; AB1256.
DR   RefSeq; NP_464975.1; NC_003210.1.
DR   RefSeq; WP_003721956.1; NZ_CP023861.1.
DR   AlphaFoldDB; Q8Y755; -.
DR   SMR; Q8Y755; -.
DR   STRING; 169963.lmo1450; -.
DR   PaxDb; Q8Y755; -.
DR   EnsemblBacteria; CAC99528; CAC99528; CAC99528.
DR   GeneID; 66483994; -.
DR   GeneID; 987133; -.
DR   KEGG; lmo:lmo1450; -.
DR   PATRIC; fig|169963.11.peg.1489; -.
DR   eggNOG; COG0513; Bacteria.
DR   HOGENOM; CLU_003041_1_3_9; -.
DR   OMA; IKPICRK; -.
DR   PhylomeDB; Q8Y755; -.
DR   BioCyc; LMON169963:LMO1450-MON; -.
DR   PHI-base; PHI:7619; -.
DR   Proteomes; UP000000817; Chromosome.
DR   GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR   GO; GO:0003723; F:RNA binding; IBA:GO_Central.
DR   GO; GO:0003724; F:RNA helicase activity; IBA:GO_Central.
DR   GO; GO:0033592; F:RNA strand annealing activity; IBA:GO_Central.
DR   GO; GO:0009409; P:response to cold; IEA:InterPro.
DR   GO; GO:0000027; P:ribosomal large subunit assembly; IBA:GO_Central.
DR   GO; GO:0006401; P:RNA catabolic process; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.40.50.300; -; 2.
DR   HAMAP; MF_01494; DEAD_helicase_CshB; 1.
DR   InterPro; IPR030881; CshB.
DR   InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR   InterPro; IPR014001; Helicase_ATP-bd.
DR   InterPro; IPR001650; Helicase_C.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR014014; RNA_helicase_DEAD_Q_motif.
DR   Pfam; PF00270; DEAD; 1.
DR   Pfam; PF00271; Helicase_C; 1.
DR   SMART; SM00487; DEXDc; 1.
DR   SMART; SM00490; HELICc; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR   PROSITE; PS51194; HELICASE_CTER; 1.
DR   PROSITE; PS51195; Q_MOTIF; 1.
PE   2: Evidence at transcript level;
KW   ATP-binding; Cytoplasm; Helicase; Hydrolase; Nucleotide-binding;
KW   Reference proteome; RNA-binding; Stress response.
FT   CHAIN           1..435
FT                   /note="DEAD-box ATP-dependent RNA helicase CshB"
FT                   /id="PRO_0000430110"
FT   DOMAIN          36..208
FT                   /note="Helicase ATP-binding"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01494"
FT   DOMAIN          235..378
FT                   /note="Helicase C-terminal"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01494"
FT   REGION          383..435
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOTIF           5..33
FT                   /note="Q motif"
FT   MOTIF           156..159
FT                   /note="DEAD box"
FT   COMPBIAS        383..404
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   BINDING         49..56
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01494"
SQ   SEQUENCE   435 AA;  49651 MW;  FBEDDD4E4977684F CRC64;
     MTKKSRFDQF GFQPFIGLAI DKLGFYEPTE VQQKLIPGIL KGESIIGQSQ TGTGKTHTFI
     LPIINNVNPE KDAVQAVITA PSRELATQIY NEIRKVTKYS EKEIAVQLVI GGTDKQRAID
     KLKKQPQIIV GTPGRINDLI REQALFVHTA KTLVIDEADM TLDMGFLNDV DHIAGKMPAN
     LQMLVFSATI PQKLKPFLSK YMENPRYEHI QPKVAASKTV EHRIMATRSR NKLDLLKNVL
     VGSQPYLAIV FTNTKTTADE VANGLIERGL KVAKIHGDVN PRERKRTMKQ IENLDYQYVV
     ATDLAARGID IQGISHVVNY ELPDDLDFYI HRTGRTGRAG HSGIALTLFE PADEDRLNQL
     EKMGIEFKHV DWKNKEFVTL EDRNRRAKRE AKRETADPRE IGMRKKAKQK GKPNYKKKIN
     YKMNEIKRRE RRKKR