ID   CSK_CALVO               Reviewed;         140 AA.
AC   Q7M3V5;
DT   10-MAY-2004, integrated into UniProtKB/Swiss-Prot.
DT   15-DEC-2003, sequence version 1.
DT   25-MAY-2022, entry version 38.
DE   RecName: Full=Callisulfakinin;
DE   Contains:
DE     RecName: Full=Callisulfakinin-1;
DE     AltName: Full=Callisulfakinin I;
DE   Contains:
DE     RecName: Full=Callisulfakinin-2;
DE     AltName: Full=Callisulfakinin II;
DE   Flags: Precursor;
OS   Calliphora vomitoria (Blue bottle fly) (Musca vomitoria).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Oestroidea;
OC   Calliphoridae; Calliphorinae; Calliphora.
OX   NCBI_TaxID=27454;
RN   [1]
RP   NUCLEOTIDE SEQUENCE, PROTEIN SEQUENCE OF 111-119, SULFATION AT TYR-114 AND
RP   TYR-131, AMIDATION AT PHE-119 AND PHE-136, AND TISSUE SPECIFICITY.
RC   TISSUE=Head;
RX   PubMed=7556217; DOI=10.1111/j.1432-1033.1995.tb20854.x;
RA   Duve H., Thorpe A., Scott A.G., Johnsen A.H., Rehfeld J.F., Hines E.,
RA   East P.D.;
RT   "The sulfakinins of the blowfly Calliphora vomitoria. Peptide isolation,
RT   gene cloning and expression studies.";
RL   Eur. J. Biochem. 232:633-640(1995).
CC   -!- FUNCTION: Callisulfakinin I is a neuropeptide. The existence of
CC       Callisulfakinin II is uncertain.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- TISSUE SPECIFICITY: In brain, it is specifically expressed in four
CC       pairs of neurons. Not expressed in other cells of the brain and in the
CC       thoracico-abdominal ganglion. {ECO:0000269|PubMed:7556217}.
CC   -!- SIMILARITY: Belongs to the gastrin/cholecystokinin family.
CC       {ECO:0000305}.
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DR   PIR; S66610; S66610.
DR   AlphaFoldDB; Q7M3V5; -.
DR   PRIDE; Q7M3V5; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0007218; P:neuropeptide signaling pathway; IEA:UniProtKB-KW.
DR   InterPro; IPR013152; Gastrin/cholecystokinin_CS.
DR   InterPro; IPR013259; Sulfakinin.
DR   Pfam; PF08257; Sulfakinin; 2.
DR   PROSITE; PS00259; GASTRIN; 2.
PE   1: Evidence at protein level;
KW   Amidation; Cleavage on pair of basic residues; Direct protein sequencing;
KW   Neuropeptide; Secreted; Signal; Sulfation.
FT   SIGNAL          1..30
FT                   /evidence="ECO:0000255"
FT   PROPEP          31..109
FT                   /id="PRO_0000010662"
FT   PEPTIDE         111..119
FT                   /note="Callisulfakinin-1"
FT                   /id="PRO_0000010663"
FT   PEPTIDE         123..136
FT                   /note="Callisulfakinin-2"
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_0000010664"
FT   PROPEP          139..140
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_0000010665"
FT   MOD_RES         114
FT                   /note="Sulfotyrosine"
FT                   /evidence="ECO:0000269|PubMed:7556217"
FT   MOD_RES         119
FT                   /note="Phenylalanine amide"
FT                   /evidence="ECO:0000269|PubMed:7556217"
FT   MOD_RES         131
FT                   /note="Sulfotyrosine"
FT                   /evidence="ECO:0000269|PubMed:7556217"
FT   MOD_RES         136
FT                   /note="Phenylalanine amide"
FT                   /evidence="ECO:0000269|PubMed:7556217"
SQ   SEQUENCE   140 AA;  16352 MW;  2DDB3AB0259F404B CRC64;
     MYSQQRIFNS KYFIFFIAVL SIFWLPTMSA RNLENSKNEN GISGSNSGNG KMNSQYNTGS
     PSAYYSAKHN LRSMLMAPKD YQQKLHAKIP LNLDLMDFLL EYEDEDRSKR FDDYGHMRFG
     KRGGEEQFDD YGHMRFGRSI