CSLA1_ORYSJ
ID CSLA1_ORYSJ Reviewed; 521 AA.
AC Q7PC76; B7ETH8;
DT 26-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT 15-DEC-2003, sequence version 1.
DT 03-AUG-2022, entry version 115.
DE RecName: Full=Glucomannan 4-beta-mannosyltransferase 1 {ECO:0000305};
DE EC=2.4.1.32 {ECO:0000269|PubMed:17307900};
DE AltName: Full=Cellulose synthase-like protein A1 {ECO:0000303|PubMed:11842136};
DE Short=OsCslA1 {ECO:0000303|PubMed:11842136};
DE AltName: Full=Glucomannan-synthase 1 {ECO:0000305};
DE Short=Mannan synthase 1 {ECO:0000305};
GN Name=CSLA1 {ECO:0000303|PubMed:11842136};
GN OrderedLocusNames=Os02g0192500, LOC_Os02g09930;
GN ORFNames=P0437H03.110, P0453H10.31;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
RN [5]
RP IDENTIFICATION, GENE FAMILY, AND NOMENCLATURE.
RX PubMed=11842136; DOI=10.1104/pp.010875;
RA Hazen S.P., Scott-Craig J.S., Walton J.D.;
RT "Cellulose synthase-like genes of rice.";
RL Plant Physiol. 128:336-340(2002).
RN [6]
RP FUNCTION, AND CATALYTIC ACTIVITY.
RX PubMed=17307900; DOI=10.1104/pp.106.093989;
RA Liepman A.H., Nairn C.J., Willats W.G.T., Soerensen I., Roberts A.W.,
RA Keegstra K.;
RT "Functional genomic analysis supports conservation of function among
RT cellulose synthase-like a gene family members and suggests diverse roles of
RT mannans in plants.";
RL Plant Physiol. 143:1881-1893(2007).
CC -!- FUNCTION: Possesses glucomannan synthase and mannan synthase activities
CC in vitro. Mannan synthase consists of a 4-beta-mannosyltransferase
CC activity on mannan using GDP-mannose. The beta-1,4-mannan product is
CC the backbone for galactomannan synthesis by galactomannan
CC galactosyltransferase. Galactomannan is a noncellulosic polysaccharides
CC of plant cell wall. {ECO:0000269|PubMed:17307900}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=GDP-mannose + (glucomannan)n = GDP + (glucomannan)n+1.;
CC EC=2.4.1.32; Evidence={ECO:0000269|PubMed:17307900};
CC -!- SUBCELLULAR LOCATION: Golgi apparatus membrane {ECO:0000305}; Multi-
CC pass membrane protein {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the glycosyltransferase 2 family. Plant
CC cellulose synthase-like A subfamily. {ECO:0000305}.
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DR EMBL; AP000366; BAD15390.1; -; Genomic_DNA.
DR EMBL; AP005785; BAD34025.1; -; Genomic_DNA.
DR EMBL; AP008208; BAF08078.1; -; Genomic_DNA.
DR EMBL; AP014958; BAS77418.1; -; Genomic_DNA.
DR EMBL; AK102694; BAG95675.1; -; mRNA.
DR EMBL; BK000080; DAA01743.1; -; Genomic_DNA.
DR RefSeq; XP_015625335.1; XM_015769849.1.
DR AlphaFoldDB; Q7PC76; -.
DR STRING; 4530.OS02T0192500-01; -.
DR CAZy; GT2; Glycosyltransferase Family 2.
DR PaxDb; Q7PC76; -.
DR PRIDE; Q7PC76; -.
DR EnsemblPlants; Os02t0192500-01; Os02t0192500-01; Os02g0192500.
DR GeneID; 4328585; -.
DR Gramene; Os02t0192500-01; Os02t0192500-01; Os02g0192500.
DR KEGG; osa:4328585; -.
DR eggNOG; ENOG502QR7J; Eukaryota.
DR InParanoid; Q7PC76; -.
DR OMA; ETEASCH; -.
DR OrthoDB; 559375at2759; -.
DR Proteomes; UP000000763; Chromosome 2.
DR Proteomes; UP000059680; Chromosome 2.
DR ExpressionAtlas; Q7PC76; baseline and differential.
DR Genevisible; Q7PC76; OS.
DR GO; GO:0005794; C:Golgi apparatus; IBA:GO_Central.
DR GO; GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0047259; F:glucomannan 4-beta-mannosyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0016757; F:glycosyltransferase activity; IBA:GO_Central.
DR GO; GO:0051753; F:mannan synthase activity; IBA:GO_Central.
DR GO; GO:0071555; P:cell wall organization; IEA:UniProtKB-KW.
DR Gene3D; 3.90.550.10; -; 1.
DR InterPro; IPR001173; Glyco_trans_2-like.
DR InterPro; IPR029044; Nucleotide-diphossugar_trans.
DR Pfam; PF13632; Glyco_trans_2_3; 1.
DR SUPFAM; SSF53448; SSF53448; 1.
PE 1: Evidence at protein level;
KW Cell wall biogenesis/degradation; Glycosyltransferase; Golgi apparatus;
KW Membrane; Reference proteome; Transferase; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..521
FT /note="Glucomannan 4-beta-mannosyltransferase 1"
FT /id="PRO_0000319372"
FT TRANSMEM 22..42
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 355..375
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 391..411
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 471..491
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 495..515
FT /note="Helical"
FT /evidence="ECO:0000255"
FT ACT_SITE 123
FT /evidence="ECO:0000255"
FT ACT_SITE 276
FT /evidence="ECO:0000255"
FT BINDING 182
FT /ligand="substrate"
FT /evidence="ECO:0000255"
FT BINDING 184
FT /ligand="substrate"
FT /evidence="ECO:0000255"
SQ SEQUENCE 521 AA; 59447 MW; 854A40E268B6D98E CRC64;
MEVNGGGAAG LPEAWSQVRA PVIVPLLRLA VAVCLTMSVL LFLERMYMAV VISGVKILRR
RPDRRYRCDP IPDDDPELGT SAFPVVLIQI PMFNEREVYQ LSIGAVCGLS WPSDRLVVQV
LDDSTDPVIK EMVRIECERW AHKGVNITYQ IRENRKGYKA GALKEGMKHG YVRECEYVAI
FDADFQPDPD FLRRTIPFLV HNSDIALVQA RWRFVNADEC LMTRMQEMSL DYHFTVEQEV
SSSVCAFFGF NGTAGVWRVS AVNEAGGWKD RTTVEDMDLA IRASLKGWKF VYLGDVQVKS
ELPSTFKAFR FQQHRWSCGP ANLFRKMLME IVRNKKVTIW KKIHVIYNFF LIRKIIAHIV
TFAFYCLIIP ATIFVPEVRI PKWGCVYIPT IITLLNSVGT PRSFHLLFFW ILFENVMSLH
RTKATLIGLL EAGRANEWVV TEKLGNALKM KSSSKSSAKK SFMRVWDRLN VTELGVAAFL
FSCGWYDLAF GKDHFFIYLF FQGAAFFIVG IGYVGTIVPQ S
