CSLA2_ORYSJ
ID CSLA2_ORYSJ Reviewed; 580 AA.
AC Q7PC67; Q0IXV9; Q7XEU7; Q8W1N9; Q948I4;
DT 26-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT 26-FEB-2008, sequence version 2.
DT 03-AUG-2022, entry version 86.
DE RecName: Full=Probable glucomannan 4-beta-mannosyltransferase 2 {ECO:0000305};
DE EC=2.4.1.32 {ECO:0000250|UniProtKB:Q7PC76};
DE AltName: Full=Cellulose synthase-like protein A2 {ECO:0000303|PubMed:11842136};
DE Short=OsCslA2 {ECO:0000303|PubMed:11842136};
DE AltName: Full=Glucomannan synthase {ECO:0000305};
DE AltName: Full=Mannan synthase 2 {ECO:0000305};
GN Name=CSLA2 {ECO:0000303|PubMed:11842136};
GN OrderedLocusNames=Os10g0406400, LOC_Os10g26630; ORFNames=OSJNBa0060A14.12;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12791992; DOI=10.1126/science.1083523;
RA Yu Y., Rambo T., Currie J., Saski C., Kim H.-R., Collura K., Thompson S.,
RA Simmons J., Yang T.-J., Nah G., Patel A.J., Thurmond S., Henry D.,
RA Oates R., Palmer M., Pries G., Gibson J., Anderson H., Paradkar M.,
RA Crane L., Dale J., Carver M.B., Wood T., Frisch D., Engler F.,
RA Soderlund C., Palmer L.E., Teytelman L., Nascimento L., De la Bastide M.,
RA Spiegel L., Ware D., O'Shaughnessy A., Dike S., Dedhia N., Preston R.,
RA Huang E., Ferraro K., Kuit K., Miller B., Zutavern T., Katzenberger F.,
RA Muller S., Balija V., Martienssen R.A., Stein L., Minx P., Johnson D.,
RA Cordum H., Mardis E., Cheng Z., Jiang J., Wilson R., McCombie W.R.,
RA Wing R.A., Yuan Q., Ouyang S., Liu J., Jones K.M., Gansberger K.,
RA Moffat K., Hill J., Tsitrin T., Overton L., Bera J., Kim M., Jin S.,
RA Tallon L., Ciecko A., Pai G., Van Aken S., Utterback T., Reidmuller S.,
RA Bormann J., Feldblyum T., Hsiao J., Zismann V., Blunt S., de Vazeille A.R.,
RA Shaffer T., Koo H., Suh B., Yang Q., Haas B., Peterson J., Pertea M.,
RA Volfovsky N., Wortman J., White O., Salzberg S.L., Fraser C.M., Buell C.R.,
RA Messing J., Song R., Fuks G., Llaca V., Kovchak S., Young S., Bowers J.E.,
RA Paterson A.H., Johns M.A., Mao L., Pan H., Dean R.A.;
RT "In-depth view of structure, activity, and evolution of rice chromosome
RT 10.";
RL Science 300:1566-1569(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [5]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 317-580, IDENTIFICATION, GENE FAMILY, AND
RP NOMENCLATURE.
RX PubMed=11842136; DOI=10.1104/pp.010875;
RA Hazen S.P., Scott-Craig J.S., Walton J.D.;
RT "Cellulose synthase-like genes of rice.";
RL Plant Physiol. 128:336-340(2002).
CC -!- FUNCTION: Probable mannan synthase which consists of a 4-beta-
CC mannosyltransferase activity on mannan using GDP-mannose. The beta-1,4-
CC mannan product is the backbone for galactomannan synthesis by
CC galactomannan galactosyltransferase. Galactomannan is a noncellulosic
CC polysaccharides of plant cell wall. {ECO:0000250|UniProtKB:Q7PC76}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=GDP-mannose + (glucomannan)n = GDP + (glucomannan)n+1.;
CC EC=2.4.1.32; Evidence={ECO:0000250|UniProtKB:Q7PC76};
CC -!- SUBCELLULAR LOCATION: Golgi apparatus membrane {ECO:0000305}; Multi-
CC pass membrane protein {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the glycosyltransferase 2 family. Plant
CC cellulose synthase-like A subfamily. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAK98678.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=AAP53691.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=BAF26478.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC021893; AAK98678.1; ALT_SEQ; Genomic_DNA.
DR EMBL; DP000086; AAP53691.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AP008216; BAF26478.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AP014966; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AF435640; AAL38525.1; -; mRNA.
DR EMBL; BK000092; DAA01755.1; -; Genomic_DNA.
DR RefSeq; XP_015612871.1; XM_015757385.1.
DR AlphaFoldDB; Q7PC67; -.
DR SMR; Q7PC67; -.
DR STRING; 4530.OS10T0406400-00; -.
DR CAZy; GT2; Glycosyltransferase Family 2.
DR PaxDb; Q7PC67; -.
DR PRIDE; Q7PC67; -.
DR GeneID; 4348586; -.
DR KEGG; osa:4348586; -.
DR eggNOG; ENOG502SHF0; Eukaryota.
DR InParanoid; Q7PC67; -.
DR OrthoDB; 559375at2759; -.
DR Proteomes; UP000000763; Chromosome 10.
DR Proteomes; UP000059680; Chromosome 10.
DR GO; GO:0005794; C:Golgi apparatus; IBA:GO_Central.
DR GO; GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0047259; F:glucomannan 4-beta-mannosyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0016757; F:glycosyltransferase activity; IBA:GO_Central.
DR GO; GO:0051753; F:mannan synthase activity; IBA:GO_Central.
DR GO; GO:0071555; P:cell wall organization; IEA:UniProtKB-KW.
DR Gene3D; 3.90.550.10; -; 1.
DR InterPro; IPR001173; Glyco_trans_2-like.
DR InterPro; IPR029044; Nucleotide-diphossugar_trans.
DR Pfam; PF13632; Glyco_trans_2_3; 1.
DR SUPFAM; SSF53448; SSF53448; 1.
PE 2: Evidence at transcript level;
KW Cell wall biogenesis/degradation; Glycosyltransferase; Golgi apparatus;
KW Membrane; Reference proteome; Transferase; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..580
FT /note="Probable glucomannan 4-beta-mannosyltransferase 2"
FT /id="PRO_0000319373"
FT TRANSMEM 85..105
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 414..434
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 437..457
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 528..548
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 554..574
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 1..33
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..30
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 182
FT /evidence="ECO:0000255"
FT ACT_SITE 335
FT /evidence="ECO:0000255"
FT BINDING 241
FT /ligand="substrate"
FT /evidence="ECO:0000255"
FT BINDING 243
FT /ligand="substrate"
FT /evidence="ECO:0000255"
SQ SEQUENCE 580 AA; 65341 MW; 9D8ABF4975DBFA5B CRC64;
MSTNGGAPSQ KRSWLPSRPL LTTTTQTYPP PLLPFKKLHA PPTAARRSLP PAASKPMASS
SSSSLPAAWA AAVRAWAVAP ALRAAVWACL AMSAMLVAEA AWMGLASLAA AAARRLRGYG
YRWEPMAAPP DVEAPAPAPA EFPMVLVQIP MYNEKEVYKL SIGAACALTW PPDRIIIQVL
DDSTDPFVKE LVELECKEWA SKKINIKYEV RNNRKGYKAG ALRKGMEHTY AQLCDFVAIF
DADFEPESDF LLKTMPYLLH NPKIALVQTR WEFVNYNVCL MTRIQKMSLD YHFKVEQESG
SFMHAFFGFN GTAGVWRVSA INQSGGWKDR TTVEDMDLAV RASLKGWEFL YVGDIRVKSE
LPSTFQAYRH QQHRWTCGAA NLFRKMAWEI ITNKEVSMWK KYHLLYSFFF VRRAIAPILT
FLFYCIVIPL SAMVPEVTIP VWGLVYIPTA ITIMNAIRNP GSVHLMPFWI LFENVMAMHR
MRAALSGLLE TARANDWVVT EKVGDQVKDE LDVPLLEPLK PTECAERIYI PELLLALYLL
ICASYDFVLG NHKYYIYIYL QAVAFTVMGF GFVGTRTPCS
