CSLA3_ORYSJ
ID CSLA3_ORYSJ Reviewed; 551 AA.
AC Q67X45; Q0DDE0; Q7PC75;
DT 26-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT 11-OCT-2004, sequence version 1.
DT 03-AUG-2022, entry version 71.
DE RecName: Full=Probable glucomannan 4-beta-mannosyltransferase 3 {ECO:0000305};
DE EC=2.4.1.32 {ECO:0000250|UniProtKB:Q7PC76};
DE AltName: Full=Cellulose synthase-like protein A3 {ECO:0000303|PubMed:11842136};
DE Short=OsCslA3 {ECO:0000303|PubMed:11842136};
DE AltName: Full=Glucomannan synthase {ECO:0000305};
DE AltName: Full=Mannan synthase 3 {ECO:0000305};
GN Name=CSLA3 {ECO:0000303|PubMed:11842136};
GN OrderedLocusNames=Os06g0230100, LOC_Os06g12460; ORFNames=P0525F01.26;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [4]
RP IDENTIFICATION, GENE FAMILY, AND NOMENCLATURE.
RX PubMed=11842136; DOI=10.1104/pp.010875;
RA Hazen S.P., Scott-Craig J.S., Walton J.D.;
RT "Cellulose synthase-like genes of rice.";
RL Plant Physiol. 128:336-340(2002).
CC -!- FUNCTION: Probable mannan synthase which consists of a 4-beta-
CC mannosyltransferase activity on mannan using GDP-mannose. The beta-1,4-
CC mannan product is the backbone for galactomannan synthesis by
CC galactomannan galactosyltransferase. Galactomannan is a noncellulosic
CC polysaccharides of plant cell wall. {ECO:0000250|UniProtKB:Q7PC76}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=GDP-mannose + (glucomannan)n = GDP + (glucomannan)n+1.;
CC EC=2.4.1.32; Evidence={ECO:0000250|UniProtKB:Q7PC76};
CC -!- SUBCELLULAR LOCATION: Golgi apparatus membrane {ECO:0000305}; Multi-
CC pass membrane protein {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the glycosyltransferase 2 family. Plant
CC cellulose synthase-like A subfamily. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAF19133.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AP003509; BAD37274.1; -; Genomic_DNA.
DR EMBL; AP008212; BAF19133.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AP014962; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BK000081; DAA01744.1; -; Genomic_DNA.
DR RefSeq; XP_015644248.1; XM_015788762.1.
DR AlphaFoldDB; Q67X45; -.
DR STRING; 4530.OS06T0230100-00; -.
DR CAZy; GT2; Glycosyltransferase Family 2.
DR PRIDE; Q67X45; -.
DR GeneID; 4340570; -.
DR KEGG; osa:4340570; -.
DR InParanoid; Q67X45; -.
DR OrthoDB; 559375at2759; -.
DR Proteomes; UP000000763; Chromosome 6.
DR Proteomes; UP000059680; Chromosome 6.
DR GO; GO:0005794; C:Golgi apparatus; IBA:GO_Central.
DR GO; GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0047259; F:glucomannan 4-beta-mannosyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0016757; F:glycosyltransferase activity; IBA:GO_Central.
DR GO; GO:0051753; F:mannan synthase activity; IBA:GO_Central.
DR GO; GO:0071555; P:cell wall organization; IEA:UniProtKB-KW.
DR Gene3D; 3.90.550.10; -; 1.
DR InterPro; IPR001173; Glyco_trans_2-like.
DR InterPro; IPR029044; Nucleotide-diphossugar_trans.
DR Pfam; PF13632; Glyco_trans_2_3; 1.
DR SUPFAM; SSF53448; SSF53448; 1.
PE 2: Evidence at transcript level;
KW Cell wall biogenesis/degradation; Glycosyltransferase; Golgi apparatus;
KW Membrane; Reference proteome; Transferase; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..551
FT /note="Probable glucomannan 4-beta-mannosyltransferase 3"
FT /id="PRO_0000319374"
FT TRANSMEM 60..80
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 386..406
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 409..429
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 504..524
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 525..545
FT /note="Helical"
FT /evidence="ECO:0000255"
FT ACT_SITE 154
FT /evidence="ECO:0000255"
FT ACT_SITE 307
FT /evidence="ECO:0000255"
FT BINDING 213
FT /ligand="substrate"
FT /evidence="ECO:0000255"
FT BINDING 215
FT /ligand="substrate"
FT /evidence="ECO:0000255"
SQ SEQUENCE 551 AA; 61548 MW; 38076BDC650A036E CRC64;
MAMAGADGPT AGAAAAVRWR GGESLLLLLL RWPSSAELVA AWGAARASAV APALAAASAA
CLALSAMLLA DAVLMAAACF ARRRPDRRYR ATPLGAGAGA DDDDDDEEAG RVAYPMVLVQ
IPMYNEREVY KLSIGAACGL SWPSDRLIVQ VLDDSTDPTV KGLVELECKS WGNKGKNVKY
EVRNTRKGYK AGALKEGLLR DYVQQCNYVA IFDADFQPEP DFLLRTIPYL VRNPQIGLVQ
AHWEFVNTSE CLMTRIQKMT LHYHFKVEQE GGSSTFAFFG FNGTAGVWRI SALEEAGGWK
DRTTVEDMDL AVRAGLKGWK FVYLADVKVK SELPSNLKTY RHQQHRWTCG AANLFRKVGA
EILFTKEVPF WWKFYLLYSF FFVRKVVAHV VPFMLYCVVI PFSVLIPEVT VPVWGVVYVP
TTITLLHAIR NTSSIHFIPF WILFENVMSF HRTKAMFIGL LELGGVNEWV VTEKLGNGSN
TKPASQILER PPCRFWDRWT MSEILFSIFL FFCATYNLAY GGDYYFVYIY LQAIAFLVVG
IGFCGTISSN S
