ID   CSLA4_ORYSJ             Reviewed;         549 AA.
AC   Q8S7W0; A0A0P0VTN5; A3AEJ7; B9FBJ8; Q0DUS4; Q10R71; Q7PC74;
DT   26-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2002, sequence version 1.
DT   03-AUG-2022, entry version 94.
DE   RecName: Full=Probable glucomannan 4-beta-mannosyltransferase 4 {ECO:0000305};
DE            EC=2.4.1.32 {ECO:0000250|UniProtKB:Q7PC76};
DE   AltName: Full=Cellulose synthase-like protein A4 {ECO:0000303|PubMed:11842136};
DE            Short=OsCslA4 {ECO:0000303|PubMed:11842136};
DE   AltName: Full=Glucomannan synthase {ECO:0000305};
DE   AltName: Full=Mannan synthase 4 {ECO:0000305};
GN   Name=CSLA4 {ECO:0000303|PubMed:11842136};
GN   OrderedLocusNames=Os03g0169500, LOC_Os03g07350;
GN   ORFNames=OsJ_009219, OSJNBa0091P11.21;
OS   Oryza sativa subsp. japonica (Rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX   NCBI_TaxID=39947;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16109971; DOI=10.1101/gr.3869505;
RG   The rice chromosome 3 sequencing consortium;
RA   Buell C.R., Yuan Q., Ouyang S., Liu J., Zhu W., Wang A., Maiti R., Haas B.,
RA   Wortman J., Pertea M., Jones K.M., Kim M., Overton L., Tsitrin T.,
RA   Fadrosh D., Bera J., Weaver B., Jin S., Johri S., Reardon M., Webb K.,
RA   Hill J., Moffat K., Tallon L., Van Aken S., Lewis M., Utterback T.,
RA   Feldblyum T., Zismann V., Iobst S., Hsiao J., de Vazeille A.R.,
RA   Salzberg S.L., White O., Fraser C.M., Yu Y., Kim H., Rambo T., Currie J.,
RA   Collura K., Kernodle-Thompson S., Wei F., Kudrna K., Ammiraju J.S.S.,
RA   Luo M., Goicoechea J.L., Wing R.A., Henry D., Oates R., Palmer M.,
RA   Pries G., Saski C., Simmons J., Soderlund C., Nelson W., de la Bastide M.,
RA   Spiegel L., Nascimento L., Huang E., Preston R., Zutavern T., Palmer L.,
RA   O'Shaughnessy A., Dike S., McCombie W.R., Minx P., Cordum H., Wilson R.,
RA   Jin W., Lee H.R., Jiang J., Jackson S.;
RT   "Sequence, annotation, and analysis of synteny between rice chromosome 3
RT   and diverged grass species.";
RL   Genome Res. 15:1284-1291(2005).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16100779; DOI=10.1038/nature03895;
RG   International rice genome sequencing project (IRGSP);
RT   "The map-based sequence of the rice genome.";
RL   Nature 436:793-800(2005).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=18089549; DOI=10.1093/nar/gkm978;
RG   The rice annotation project (RAP);
RT   "The rice annotation project database (RAP-DB): 2008 update.";
RL   Nucleic Acids Res. 36:D1028-D1033(2008).
RN   [4]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA   Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA   Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA   Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA   Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT   "Improvement of the Oryza sativa Nipponbare reference genome using next
RT   generation sequence and optical map data.";
RL   Rice 6:4-4(2013).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA   Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA   Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA   Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA   Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA   Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA   Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA   Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA   Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA   Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA   Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA   Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA   Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA   Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA   McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT   "The genomes of Oryza sativa: a history of duplications.";
RL   PLoS Biol. 3:266-281(2005).
RN   [6]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Nipponbare;
RG   The rice full-length cDNA consortium;
RT   "Oryza sativa full length cDNA.";
RL   Submitted (SEP-2007) to the EMBL/GenBank/DDBJ databases.
RN   [7]
RP   IDENTIFICATION, GENE FAMILY, AND NOMENCLATURE.
RX   PubMed=11842136; DOI=10.1104/pp.010875;
RA   Hazen S.P., Scott-Craig J.S., Walton J.D.;
RT   "Cellulose synthase-like genes of rice.";
RL   Plant Physiol. 128:336-340(2002).
CC   -!- FUNCTION: Probable mannan synthase which consists of a 4-beta-
CC       mannosyltransferase activity on mannan using GDP-mannose. The beta-1,4-
CC       mannan product is the backbone for galactomannan synthesis by
CC       galactomannan galactosyltransferase. Galactomannan is a noncellulosic
CC       polysaccharides of plant cell wall. {ECO:0000250|UniProtKB:Q7PC76}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=GDP-mannose + (glucomannan)n = GDP + (glucomannan)n+1.;
CC         EC=2.4.1.32; Evidence={ECO:0000250|UniProtKB:Q7PC76};
CC   -!- SUBCELLULAR LOCATION: Golgi apparatus membrane {ECO:0000305}; Multi-
CC       pass membrane protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the glycosyltransferase 2 family. Plant
CC       cellulose synthase-like A subfamily. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=ABF94194.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC       Sequence=AK287855; Type=Frameshift; Evidence={ECO:0000305};
CC       Sequence=BAF11014.2; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC       Sequence=DAA01745.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC       Sequence=EEE58400.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR   EMBL; AC073556; AAL84294.1; -; Genomic_DNA.
DR   EMBL; DP000009; ABF94194.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; AP008209; BAF11014.2; ALT_SEQ; Genomic_DNA.
DR   EMBL; AP014959; BAS82512.1; -; Genomic_DNA.
DR   EMBL; CM000140; EEE58400.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; AK287855; -; NOT_ANNOTATED_CDS; mRNA.
DR   EMBL; BK000082; DAA01745.1; ALT_SEQ; Genomic_DNA.
DR   RefSeq; XP_015630733.1; XM_015775247.1.
DR   AlphaFoldDB; Q8S7W0; -.
DR   STRING; 4530.OS03T0169500-01; -.
DR   CAZy; GT2; Glycosyltransferase Family 2.
DR   PaxDb; Q8S7W0; -.
DR   PRIDE; Q8S7W0; -.
DR   EnsemblPlants; Os03t0169500-01; Os03t0169500-01; Os03g0169500.
DR   GeneID; 4331764; -.
DR   Gramene; Os03t0169500-01; Os03t0169500-01; Os03g0169500.
DR   KEGG; osa:4331764; -.
DR   eggNOG; ENOG502SHF0; Eukaryota.
DR   HOGENOM; CLU_012856_2_0_1; -.
DR   InParanoid; Q8S7W0; -.
DR   OMA; FAVWACM; -.
DR   OrthoDB; 559375at2759; -.
DR   Proteomes; UP000000763; Chromosome 3.
DR   Proteomes; UP000007752; Chromosome 3.
DR   Proteomes; UP000059680; Chromosome 3.
DR   Genevisible; Q8S7W0; OS.
DR   GO; GO:0005794; C:Golgi apparatus; IBA:GO_Central.
DR   GO; GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0047259; F:glucomannan 4-beta-mannosyltransferase activity; IEA:UniProtKB-EC.
DR   GO; GO:0016757; F:glycosyltransferase activity; IBA:GO_Central.
DR   GO; GO:0051753; F:mannan synthase activity; IBA:GO_Central.
DR   GO; GO:0071555; P:cell wall organization; IEA:UniProtKB-KW.
DR   Gene3D; 3.90.550.10; -; 1.
DR   InterPro; IPR001173; Glyco_trans_2-like.
DR   InterPro; IPR029044; Nucleotide-diphossugar_trans.
DR   Pfam; PF13632; Glyco_trans_2_3; 1.
DR   SUPFAM; SSF53448; SSF53448; 1.
PE   2: Evidence at transcript level;
KW   Cell wall biogenesis/degradation; Glycosyltransferase; Golgi apparatus;
KW   Membrane; Reference proteome; Transferase; Transmembrane;
KW   Transmembrane helix.
FT   CHAIN           1..549
FT                   /note="Probable glucomannan 4-beta-mannosyltransferase 4"
FT                   /id="PRO_0000319375"
FT   TRANSMEM        35..55
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        383..403
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        406..426
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        497..517
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        523..543
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   ACT_SITE        151
FT                   /evidence="ECO:0000255"
FT   ACT_SITE        304
FT                   /evidence="ECO:0000255"
FT   BINDING         210
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000255"
FT   BINDING         212
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   549 AA;  61889 MW;  7F3B04BDD14DEA38 CRC64;
     MEGQWGRWRL AAAAAASSSG DQIAAAWAVV RARAVAPVLQ FAVWACMAMS VMLVLEVAYM
     SLVSLVAVKL LRRVPERRYK WEPITTGSGG VGGGDGEDEE AATGGREAAA FPMVLVQIPM
     YNEKEVYKLS IGAACALTWP PDRIIIQVLD DSTDPAIKDL VELECKDWAR KEINIKYEIR
     DNRKGYKAGA LKKGMEHIYT QQCDFVAIFD ADFQPESDFL LKTIPFLVHN PKIGLVQTRW
     EFVNYDVCLM TRIQKMSLDY HFKVEQESGS SMHSFFGFNG TAGVWRVSAI NEAGGWKDRT
     TVEDMDLAVR ASLKGWQFLY VGDIRVKSEL PSTFKAYRHQ QHRWTCGAAN LFRKMATEIA
     KNKGVSVWKK LHLLYSFFFV RRVVAPILTF LFYCVVIPLS VMVPEVSIPV WGMVYIPTAI
     TIMNAIRNPG SIHLMPFWIL FENVMAMHRM RAALTGLLET MNVNQWVVTE KVGDHVKDKL
     EVPLLEPLKP TDCVERIYIP ELMVAFYLLV CASYDLVLGA KHYYLYIYLQ AFAFIALGFG
     FAGTSTPCS