ID   CSLA5_ORYSJ             Reviewed;         574 AA.
AC   Q7PC73; B7F3Q6; Q8S7F4;
DT   26-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT   15-DEC-2003, sequence version 1.
DT   03-AUG-2022, entry version 101.
DE   RecName: Full=Probable glucomannan 4-beta-mannosyltransferase 5 {ECO:0000305};
DE            EC=2.4.1.32 {ECO:0000250|UniProtKB:Q7PC76};
DE   AltName: Full=Cellulose synthase-like protein A5 {ECO:0000303|PubMed:11842136};
DE            Short=OsCslA5 {ECO:0000303|PubMed:11842136};
DE   AltName: Full=Glucomannan synthase {ECO:0000305};
DE   AltName: Full=Mannan synthase 5 {ECO:0000305};
GN   Name=CSLA5 {ECO:0000303|PubMed:11842136};
GN   OrderedLocusNames=Os03g0377700, LOC_Os03g26044;
GN   ORFNames=OsJ_11041 {ECO:0000312|EMBL:EEE59143.1}, OSJNBb0048D20.18;
OS   Oryza sativa subsp. japonica (Rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX   NCBI_TaxID=39947;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16109971; DOI=10.1101/gr.3869505;
RG   The rice chromosome 3 sequencing consortium;
RA   Buell C.R., Yuan Q., Ouyang S., Liu J., Zhu W., Wang A., Maiti R., Haas B.,
RA   Wortman J., Pertea M., Jones K.M., Kim M., Overton L., Tsitrin T.,
RA   Fadrosh D., Bera J., Weaver B., Jin S., Johri S., Reardon M., Webb K.,
RA   Hill J., Moffat K., Tallon L., Van Aken S., Lewis M., Utterback T.,
RA   Feldblyum T., Zismann V., Iobst S., Hsiao J., de Vazeille A.R.,
RA   Salzberg S.L., White O., Fraser C.M., Yu Y., Kim H., Rambo T., Currie J.,
RA   Collura K., Kernodle-Thompson S., Wei F., Kudrna K., Ammiraju J.S.S.,
RA   Luo M., Goicoechea J.L., Wing R.A., Henry D., Oates R., Palmer M.,
RA   Pries G., Saski C., Simmons J., Soderlund C., Nelson W., de la Bastide M.,
RA   Spiegel L., Nascimento L., Huang E., Preston R., Zutavern T., Palmer L.,
RA   O'Shaughnessy A., Dike S., McCombie W.R., Minx P., Cordum H., Wilson R.,
RA   Jin W., Lee H.R., Jiang J., Jackson S.;
RT   "Sequence, annotation, and analysis of synteny between rice chromosome 3
RT   and diverged grass species.";
RL   Genome Res. 15:1284-1291(2005).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16100779; DOI=10.1038/nature03895;
RG   International rice genome sequencing project (IRGSP);
RT   "The map-based sequence of the rice genome.";
RL   Nature 436:793-800(2005).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=18089549; DOI=10.1093/nar/gkm978;
RG   The rice annotation project (RAP);
RT   "The rice annotation project database (RAP-DB): 2008 update.";
RL   Nucleic Acids Res. 36:D1028-D1033(2008).
RN   [4]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA   Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA   Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA   Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA   Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT   "Improvement of the Oryza sativa Nipponbare reference genome using next
RT   generation sequence and optical map data.";
RL   Rice 6:4-4(2013).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA   Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA   Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA   Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA   Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA   Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA   Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA   Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA   Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA   Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA   Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA   Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA   Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA   Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA   McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT   "The genomes of Oryza sativa: a history of duplications.";
RL   PLoS Biol. 3:266-281(2005).
RN   [6]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=12869764; DOI=10.1126/science.1081288;
RG   The rice full-length cDNA consortium;
RT   "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT   japonica rice.";
RL   Science 301:376-379(2003).
RN   [7]
RP   IDENTIFICATION, GENE FAMILY, AND NOMENCLATURE.
RX   PubMed=11842136; DOI=10.1104/pp.010875;
RA   Hazen S.P., Scott-Craig J.S., Walton J.D.;
RT   "Cellulose synthase-like genes of rice.";
RL   Plant Physiol. 128:336-340(2002).
CC   -!- FUNCTION: Probable mannan synthase which consists of a 4-beta-
CC       mannosyltransferase activity on mannan using GDP-mannose. The beta-1,4-
CC       mannan product is the backbone for galactomannan synthesis by
CC       galactomannan galactosyltransferase. Galactomannan is a noncellulosic
CC       polysaccharides of plant cell wall. {ECO:0000250|UniProtKB:Q7PC76}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=GDP-mannose + (glucomannan)n = GDP + (glucomannan)n+1.;
CC         EC=2.4.1.32; Evidence={ECO:0000250|UniProtKB:Q7PC76};
CC   -!- SUBCELLULAR LOCATION: Golgi apparatus membrane {ECO:0000305}; Multi-
CC       pass membrane protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the glycosyltransferase 2 family. Plant
CC       cellulose synthase-like A subfamily. {ECO:0000305}.
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DR   EMBL; AC084766; AAL82530.1; -; Genomic_DNA.
DR   EMBL; DP000009; ABF96237.1; -; Genomic_DNA.
DR   EMBL; AP008209; BAF12143.1; -; Genomic_DNA.
DR   EMBL; AP014959; BAS84424.1; -; Genomic_DNA.
DR   EMBL; CM000140; EEE59143.1; -; Genomic_DNA.
DR   EMBL; AK111424; BAG99253.1; -; mRNA.
DR   EMBL; BK000083; DAA01746.1; -; Genomic_DNA.
DR   RefSeq; XP_015627865.1; XM_015772379.1.
DR   AlphaFoldDB; Q7PC73; -.
DR   STRING; 4530.OS03T0377700-01; -.
DR   CAZy; GT2; Glycosyltransferase Family 2.
DR   PaxDb; Q7PC73; -.
DR   PRIDE; Q7PC73; -.
DR   EnsemblPlants; Os03t0377700-01; Os03t0377700-01; Os03g0377700.
DR   GeneID; 4332966; -.
DR   Gramene; Os03t0377700-01; Os03t0377700-01; Os03g0377700.
DR   KEGG; osa:4332966; -.
DR   eggNOG; ENOG502QVIM; Eukaryota.
DR   HOGENOM; CLU_012856_2_0_1; -.
DR   InParanoid; Q7PC73; -.
DR   OMA; ELKWPKE; -.
DR   OrthoDB; 559375at2759; -.
DR   Proteomes; UP000000763; Chromosome 3.
DR   Proteomes; UP000007752; Chromosome 3.
DR   Proteomes; UP000059680; Chromosome 3.
DR   Genevisible; Q7PC73; OS.
DR   GO; GO:0005794; C:Golgi apparatus; IBA:GO_Central.
DR   GO; GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0047259; F:glucomannan 4-beta-mannosyltransferase activity; IEA:UniProtKB-EC.
DR   GO; GO:0016757; F:glycosyltransferase activity; IBA:GO_Central.
DR   GO; GO:0051753; F:mannan synthase activity; IBA:GO_Central.
DR   GO; GO:0071555; P:cell wall organization; IEA:UniProtKB-KW.
DR   Gene3D; 3.90.550.10; -; 1.
DR   InterPro; IPR001173; Glyco_trans_2-like.
DR   InterPro; IPR029044; Nucleotide-diphossugar_trans.
DR   Pfam; PF13632; Glyco_trans_2_3; 1.
DR   SUPFAM; SSF53448; SSF53448; 1.
PE   2: Evidence at transcript level;
KW   Cell wall biogenesis/degradation; Glycosyltransferase; Golgi apparatus;
KW   Membrane; Reference proteome; Transferase; Transmembrane;
KW   Transmembrane helix.
FT   CHAIN           1..574
FT                   /note="Probable glucomannan 4-beta-mannosyltransferase 5"
FT                   /id="PRO_0000319376"
FT   TRANSMEM        66..86
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        405..425
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        428..448
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        521..541
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        546..566
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   ACT_SITE        173
FT                   /evidence="ECO:0000255"
FT   ACT_SITE        326
FT                   /evidence="ECO:0000255"
FT   BINDING         232
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000255"
FT   BINDING         234
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   574 AA;  64004 MW;  42E591738E9B02A7 CRC64;
     MEAGEAAGAV LFLLAAAVSL LAAVSTGALD FTYLVTVVGE GSSTSPGSGG GAWWREAWVG
     ARSRAVAPAL QVGVWACMVM SVMLVVEATY NSAVSVAARL VGWRPERWFK WEPLGGGAGA
     GDEEKGEAAA AAYPMVMVQI PMYNELEVYK LSIGAVCGLK WPKERLIIQV LDDSTDAFIK
     NLVELECEDW ASKGLNIKYA TRSGRKGFKA GALKKGMEWD YAKQCEYVAI FDADFQPEPD
     FLLRTVPFLM HNQNVALVQA RWVFVNDRVS LLTRIQKTFL DYHFKAEQEA GSATFAFFSF
     NGTAGVWRTE AINDAGGWKD RTTVEDMDLA VRATLKGWKF IYLGDLRVKS ELPSTYKAYC
     RQQFRWSCGG ANLFRKMIWD VLVAKKVSSL KKIYILYSFF LVRRVVAPAV AFILYNVIIP
     VSVMIPELFL PIWGVAYIPT ALLIVTAIRN PENLHTVPLW ILFESVMSMH RLRAAVAGLL
     QLQEFNQWIV TKKVGNNAFD ENNETPLLQK SRKRLINRVN LPEIGLSVFL IFCASYNLVF
     HGKNSFYINL YLQGLAFFLL GLNCVGTLPD HCCF