CSLA9_ORYSJ
ID CSLA9_ORYSJ Reviewed; 527 AA.
AC Q67VS7; B9FQ03; Q0DAU5; Q944E5;
DT 26-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT 11-OCT-2004, sequence version 1.
DT 03-AUG-2022, entry version 94.
DE RecName: Full=Probable glucomannan 4-beta-mannosyltransferase 9 {ECO:0000305};
DE EC=2.4.1.32 {ECO:0000250|UniProtKB:Q7PC76};
DE AltName: Full=Cellulose synthase-like protein A9 {ECO:0000303|PubMed:11842136};
DE Short=OsCslA9 {ECO:0000303|PubMed:11842136};
DE AltName: Full=Glucomannan synthase {ECO:0000305};
DE AltName: Full=Mannan synthase 9 {ECO:0000305};
GN Name=CSLA9 {ECO:0000303|PubMed:11842136};
GN OrderedLocusNames=Os06g0625700, LOC_Os06g42020;
GN ORFNames=OsJ_021176, OsJ_22033 {ECO:0000312|EMBL:EEE66045.1},
GN OSJNBa0072A21.15;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], GENE FAMILY, AND NOMENCLATURE.
RX PubMed=11842136; DOI=10.1104/pp.010875;
RA Hazen S.P., Scott-Craig J.S., Walton J.D.;
RT "Cellulose synthase-like genes of rice.";
RL Plant Physiol. 128:336-340(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Nipponbare;
RG The rice full-length cDNA consortium;
RT "Oryza sativa full length cDNA.";
RL Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Probable mannan synthase which consists of a 4-beta-
CC mannosyltransferase activity on mannan using GDP-mannose. The beta-1,4-
CC mannan product is the backbone for galactomannan synthesis by
CC galactomannan galactosyltransferase. Galactomannan is a noncellulosic
CC polysaccharides of plant cell wall. {ECO:0000250|UniProtKB:Q7PC76}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=GDP-mannose + (glucomannan)n = GDP + (glucomannan)n+1.;
CC EC=2.4.1.32; Evidence={ECO:0000250|UniProtKB:Q7PC76};
CC -!- SUBCELLULAR LOCATION: Golgi apparatus membrane {ECO:0000305}; Multi-
CC pass membrane protein {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the glycosyltransferase 2 family. Plant
CC cellulose synthase-like A subfamily. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAF20028.2; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AF432499; AAL25128.1; -; Genomic_DNA.
DR EMBL; AP004737; BAD37742.1; -; Genomic_DNA.
DR EMBL; AP008212; BAF20028.2; ALT_SEQ; Genomic_DNA.
DR EMBL; AP014962; BAS98696.1; -; Genomic_DNA.
DR EMBL; CM000143; EEE66045.1; -; Genomic_DNA.
DR EMBL; AK242831; -; NOT_ANNOTATED_CDS; mRNA.
DR RefSeq; XP_015643705.1; XM_015788219.1.
DR AlphaFoldDB; Q67VS7; -.
DR STRING; 4530.OS06T0625700-01; -.
DR CAZy; GT2; Glycosyltransferase Family 2.
DR PaxDb; Q67VS7; -.
DR PRIDE; Q67VS7; -.
DR EnsemblPlants; Os06t0625700-01; Os06t0625700-01; Os06g0625700.
DR GeneID; 4341571; -.
DR Gramene; Os06t0625700-01; Os06t0625700-01; Os06g0625700.
DR KEGG; osa:4341571; -.
DR eggNOG; ENOG502QR7J; Eukaryota.
DR HOGENOM; CLU_012856_2_0_1; -.
DR InParanoid; Q67VS7; -.
DR OMA; LCGWYDY; -.
DR OrthoDB; 559375at2759; -.
DR Proteomes; UP000000763; Chromosome 6.
DR Proteomes; UP000007752; Chromosome 6.
DR Proteomes; UP000059680; Chromosome 6.
DR Genevisible; Q67VS7; OS.
DR GO; GO:0005794; C:Golgi apparatus; IBA:GO_Central.
DR GO; GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0047259; F:glucomannan 4-beta-mannosyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0016757; F:glycosyltransferase activity; IBA:GO_Central.
DR GO; GO:0051753; F:mannan synthase activity; IBA:GO_Central.
DR GO; GO:0071555; P:cell wall organization; IEA:UniProtKB-KW.
DR Gene3D; 3.90.550.10; -; 1.
DR InterPro; IPR001173; Glyco_trans_2-like.
DR InterPro; IPR029044; Nucleotide-diphossugar_trans.
DR Pfam; PF13632; Glyco_trans_2_3; 1.
DR SUPFAM; SSF53448; SSF53448; 1.
PE 2: Evidence at transcript level;
KW Cell wall biogenesis/degradation; Glycosyltransferase; Golgi apparatus;
KW Membrane; Reference proteome; Transferase; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..527
FT /note="Probable glucomannan 4-beta-mannosyltransferase 9"
FT /id="PRO_0000319379"
FT TRANSMEM 37..59
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 363..383
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 399..419
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 478..498
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 505..525
FT /note="Helical"
FT /evidence="ECO:0000255"
FT ACT_SITE 131
FT /evidence="ECO:0000255"
FT ACT_SITE 284
FT /evidence="ECO:0000255"
FT BINDING 190
FT /ligand="substrate"
FT /evidence="ECO:0000255"
FT BINDING 192
FT /ligand="substrate"
FT /evidence="ECO:0000255"
FT CONFLICT 326
FT /note="C -> W (in Ref. 6; AK242831)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 527 AA; 59726 MW; 60CC46E0953DDC45 CRC64;
MAAAGAVLPE QIAAMWEQVK APVVVPLLRL SVAACLAMSV MLFVEKVYMS VVLVGVHLFG
RRPDRRYRCD PIVAAGADND DPELADANAA FPMVLIQIPM YNEREVYKLS IGAACGLSWP
SDRVIVQVLD DSTDPVIKEM VQVECKRWES KGVRIKYEIR DNRVGYKAGA LREGMKHGYV
RDCDYVAIFD ADFQPDPDFL ARTIPFLVHN PDIALVQARW KFVNANECLM TRMQEMSLDY
HFKVEQEVGS STHAFFGFNG TAGVWRISAM NEAGGWKDRT TVEDMDLAVR AGLKGWKFVY
LGDLMVKSEL PSTFKAFRYQ QHRWSCGPAN LFRKMLVEIA TNKKVTLWKK IYVIYNFFLV
RKIIGHIVTF VFYCLVVPAT VLIPEVEIPR WGYVYLPSIV TILNSIGTPR SLHLLIFWVL
FENVMSLHRT KATLIGLLET GRVNEWVVTE KLGDALKLKL PGKAFRRPRM RIGDRVNALE
LGFSAYLSFC GCYDIAYGKG YYSLFLFLQS ITFFIIGVGY VGTIVPH
