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CSLC4_ARATH
ID   CSLC4_ARATH             Reviewed;         673 AA.
AC   Q9LJP4;
DT   26-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2000, sequence version 1.
DT   03-AUG-2022, entry version 122.
DE   RecName: Full=Xyloglucan glycosyltransferase 4 {ECO:0000303|PubMed:11027699};
DE            EC=2.4.1.- {ECO:0000269|PubMed:17488821};
DE   AltName: Full=Cellulose synthase-like protein C4 {ECO:0000303|PubMed:11027699};
DE            Short=AtCslC4 {ECO:0000303|PubMed:11027699};
DE   AltName: Full=Xyloglucan synthase 4 {ECO:0000303|PubMed:11027699};
GN   Name=CSLC4 {ECO:0000303|PubMed:11027699};
GN   OrderedLocusNames=At3g28180 {ECO:0000312|Araport:AT3G28180};
GN   ORFNames=MIG10.8 {ECO:0000312|EMBL:BAB01433.1};
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=10907853; DOI=10.1093/dnares/7.3.217;
RA   Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S.;
RT   "Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence
RT   features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC
RT   clones.";
RL   DNA Res. 7:217-221(2000).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=14593172; DOI=10.1126/science.1088305;
RA   Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA   Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA   Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA   Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA   Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA   Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA   Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA   Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA   Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA   Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA   Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA   Ecker J.R.;
RT   "Empirical analysis of transcriptional activity in the Arabidopsis
RT   genome.";
RL   Science 302:842-846(2003).
RN   [4]
RP   GENE FAMILY, AND NOMENCLATURE.
RX   PubMed=11027699; DOI=10.1104/pp.124.2.495;
RA   Richmond T.A., Somerville C.R.;
RT   "The cellulose synthase superfamily.";
RL   Plant Physiol. 124:495-498(2000).
RN   [5]
RP   FUNCTION, AND SUBCELLULAR LOCATION.
RX   PubMed=17488821; DOI=10.1073/pnas.0703133104;
RA   Cocuron J.-C., Lerouxel O., Drakakaki G., Alonso A.P., Liepman A.H.,
RA   Keegstra K., Raikhel N., Wilkerson C.G.;
RT   "A gene from the cellulose synthase-like C family encodes a beta-1,4 glucan
RT   synthase.";
RL   Proc. Natl. Acad. Sci. U.S.A. 104:8550-8555(2007).
RN   [6]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-581, AND IDENTIFICATION BY
RP   MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=19376835; DOI=10.1104/pp.109.138677;
RA   Reiland S., Messerli G., Baerenfaller K., Gerrits B., Endler A.,
RA   Grossmann J., Gruissem W., Baginsky S.;
RT   "Large-scale Arabidopsis phosphoproteome profiling reveals novel
RT   chloroplast kinase substrates and phosphorylation networks.";
RL   Plant Physiol. 150:889-903(2009).
RN   [7]
RP   INTERACTION WITH CSLC4 AND XXT5, AND SUBCELLULAR LOCATION.
RX   PubMed=22665445; DOI=10.1104/pp.112.199356;
RA   Chou Y.H., Pogorelko G., Zabotina O.A.;
RT   "Xyloglucan xylosyltransferases XXT1, XXT2, and XXT5 and the glucan
RT   synthase CSLC4 form Golgi-localized multiprotein complexes.";
RL   Plant Physiol. 159:1355-1366(2012).
RN   [8]
RP   FUNCTION, INTERACTION WITH FUT1; MUR3 AND XLT2, AND SUBCELLULAR LOCATION.
RX   PubMed=25392066; DOI=10.1093/pcp/pcu161;
RA   Chou Y.H., Pogorelko G., Young Z.T., Zabotina O.A.;
RT   "Protein-protein interactions among xyloglucan-synthesizing enzymes and
RT   formation of Golgi-localized multiprotein complexes.";
RL   Plant Cell Physiol. 56:255-267(2015).
RN   [9]
RP   FUNCTION, DISRUPTION PHENOTYPE, AND TISSUE SPECIFICITY.
RC   STRAIN=cv. Columbia;
RX   PubMed=32737163; DOI=10.1073/pnas.2007245117;
RA   Kim S.-J., Chandrasekar B., Rea A.C., Danhof L., Zemelis-Durfee S.,
RA   Thrower N., Shepard Z.S., Pauly M., Brandizzi F., Keegstra K.;
RT   "The synthesis of xyloglucan, an abundant plant cell wall polysaccharide,
RT   requires CSLC function.";
RL   Proc. Natl. Acad. Sci. U.S.A. 117:20316-20324(2020).
CC   -!- FUNCTION: Beta-1,4-glucan synthase rather involved in the synthesis of
CC       the xyloglucan backbone than cellulose. Seems to work simultaneously
CC       with xyloglucan 6-xylosyltransferase. Xyloglucan is a noncellulosic
CC       polysaccharides of plant cell wall and consists of a glucan backbone
CC       substituted by xylose, galactose and fucose (PubMed:17488821,
CC       PubMed:32737163). Associates with other xyloglucan-synthesizing enzymes
CC       to form multiprotein complexes for xyloglucan synthesis in the Golgi
CC       (PubMed:25392066). {ECO:0000269|PubMed:17488821,
CC       ECO:0000269|PubMed:25392066, ECO:0000269|PubMed:32737163}.
CC   -!- SUBUNIT: Homodimer (PubMed:22665445). Interacts with XXT5
CC       (PubMed:22665445). Interacts with FUT1, MUR3 and XLT2
CC       (PubMed:25392066). {ECO:0000269|PubMed:22665445,
CC       ECO:0000269|PubMed:25392066}.
CC   -!- SUBCELLULAR LOCATION: Golgi apparatus membrane
CC       {ECO:0000269|PubMed:22665445, ECO:0000269|PubMed:25392066,
CC       ECO:0000305|PubMed:17488821}; Multi-pass membrane protein
CC       {ECO:0000305|PubMed:17488821}.
CC   -!- TISSUE SPECIFICITY: Expressed in seedlings, roots, leaves, stems,
CC       flowers and seeds. {ECO:0000269|PubMed:32737163}.
CC   -!- DISRUPTION PHENOTYPE: Normal xyloglucan (XyG) levels (PubMed:32737163).
CC       Plants missing several xyloglucan synthases (e.g. CSLC4, CSLC5, CSLC6,
CC       CSLC8 and CSLC12) have no detectable XyG levels and several associated
CC       phenotypes including reduced stems height and leaves area, as well as
CC       shorter root hairs and reduced pollen tube formation ability
CC       (PubMed:32737163). {ECO:0000269|PubMed:32737163}.
CC   -!- SIMILARITY: Belongs to the glycosyltransferase 2 family. Plant
CC       cellulose synthase-like C subfamily. {ECO:0000305}.
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DR   EMBL; AP000415; BAB01433.1; -; Genomic_DNA.
DR   EMBL; CP002686; AEE77412.1; -; Genomic_DNA.
DR   EMBL; AY056286; AAL07135.1; -; mRNA.
DR   EMBL; AY091433; AAM14372.1; -; mRNA.
DR   RefSeq; NP_566835.1; NM_113737.3.
DR   AlphaFoldDB; Q9LJP4; -.
DR   SMR; Q9LJP4; -.
DR   BioGRID; 7773; 6.
DR   IntAct; Q9LJP4; 2.
DR   STRING; 3702.AT3G28180.1; -.
DR   CAZy; GT2; Glycosyltransferase Family 2.
DR   iPTMnet; Q9LJP4; -.
DR   PaxDb; Q9LJP4; -.
DR   PRIDE; Q9LJP4; -.
DR   ProteomicsDB; 220364; -.
DR   EnsemblPlants; AT3G28180.1; AT3G28180.1; AT3G28180.
DR   GeneID; 822444; -.
DR   Gramene; AT3G28180.1; AT3G28180.1; AT3G28180.
DR   KEGG; ath:AT3G28180; -.
DR   Araport; AT3G28180; -.
DR   TAIR; locus:2089730; AT3G28180.
DR   eggNOG; ENOG502QTBF; Eukaryota.
DR   HOGENOM; CLU_012856_1_0_1; -.
DR   InParanoid; Q9LJP4; -.
DR   OMA; STKQFTW; -.
DR   OrthoDB; 286400at2759; -.
DR   PhylomeDB; Q9LJP4; -.
DR   BioCyc; ARA:AT3G28180-MON; -.
DR   BioCyc; MetaCyc:AT3G28180-MON; -.
DR   BRENDA; 2.4.2.39; 399.
DR   PRO; PR:Q9LJP4; -.
DR   Proteomes; UP000006548; Chromosome 3.
DR   ExpressionAtlas; Q9LJP4; baseline and differential.
DR   Genevisible; Q9LJP4; AT.
DR   GO; GO:0005768; C:endosome; HDA:TAIR.
DR   GO; GO:0005794; C:Golgi apparatus; HDA:TAIR.
DR   GO; GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0000138; C:Golgi trans cisterna; HDA:TAIR.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005802; C:trans-Golgi network; HDA:TAIR.
DR   GO; GO:0016757; F:glycosyltransferase activity; IMP:UniProtKB.
DR   GO; GO:0042803; F:protein homodimerization activity; IPI:UniProtKB.
DR   GO; GO:0071555; P:cell wall organization; IMP:UniProtKB.
DR   GO; GO:0099402; P:plant organ development; IMP:UniProtKB.
DR   GO; GO:0048868; P:pollen tube development; IMP:UniProtKB.
DR   Gene3D; 3.90.550.10; -; 1.
DR   InterPro; IPR001173; Glyco_trans_2-like.
DR   InterPro; IPR029044; Nucleotide-diphossugar_trans.
DR   Pfam; PF13632; Glyco_trans_2_3; 1.
DR   SUPFAM; SSF53448; SSF53448; 1.
PE   1: Evidence at protein level;
KW   Cell wall biogenesis/degradation; Glycosyltransferase; Golgi apparatus;
KW   Membrane; Phosphoprotein; Reference proteome; Transferase; Transmembrane;
KW   Transmembrane helix.
FT   CHAIN           1..673
FT                   /note="Xyloglucan glycosyltransferase 4"
FT                   /id="PRO_0000319341"
FT   TRANSMEM        90..110
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        144..164
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        469..489
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        494..514
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        623..643
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        648..668
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   ACT_SITE        238
FT                   /evidence="ECO:0000255"
FT   ACT_SITE        391
FT                   /evidence="ECO:0000255"
FT   BINDING         297
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000255"
FT   BINDING         299
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000255"
FT   MOD_RES         581
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:19376835"
SQ   SEQUENCE   673 AA;  77516 MW;  1BC9A7E77D45D4A7 CRC64;
     MAPNSVAVTM EKPDNFSLLE INGSDPSSFP DKRKSISPKQ FSWFLLLKAH RLISCLSWLV
     SSVKKRIAFS AKNINEEEDP KSRGKQMYRF IKACLVISII ALSIEIVAHF KKWNLDLINR
     PSWEVYGLVE WSYMAWLSFR SDYIAPLVIS LSRFCTVLFL IQSLDRLVLC LGCFWIKFKK
     IEPKLTEESI DLEDPSSFPM VLIQIPMCNE REVYEQSIGA ASQLDWPKDR ILIQVLDDSD
     DPNLQLLIKE EVSVWAEKGV NIIYRHRLIR TGYKAGNLKS AMTCDYVKDY EFVTIFDADF
     TPNPDFLKKT VPHFKGNPEL GLVQARWSFV NKDENLLTRL QNINLCFHFE VEQQVNGVFL
     NFFGFNGTAG VWRIKALEES GGWLERTTVE DMDIAVRAHL NGWKFIYLND VEVTCELPES
     YEAYKKQQHR WHSGPMQLFR LCLPSIIKSK ISVWKKANLI FLFFLLRKLI LPFYSFTLFC
     IILPLTMFIP EAELPLWIIC YVPIFISLLN ILPSPKSFPF LVPYLLFENT MSITKFNAMI
     SGLFQFGSAY EWVVTKKTGR SSESDLLAFA EKEEKLHRRN SESGLELLSK LKEQETNLVG
     QETVKKSLGG LMRPKNKKKT NMVFKKELGL AFLLLTAAAR SFLSAHGLHF YFLLFQGLSF
     LVVGLDLIGE QIS
 
 
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