CSLD3_ARATH
ID CSLD3_ARATH Reviewed; 1145 AA.
AC Q9M9M4;
DT 26-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 125.
DE RecName: Full=Cellulose synthase-like protein D3;
DE Short=AtCslD3;
DE EC=2.4.1.-;
DE AltName: Full=Protein KOJAK;
GN Name=CSLD3; Synonyms=KJK; OrderedLocusNames=At3g03050; ORFNames=T17B22.26;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND
RP MUTAGENESIS OF GLU-829.
RC STRAIN=cv. Landsberg erecta;
RX PubMed=11156607; DOI=10.1101/gad.188801;
RA Favery B., Ryan E., Foreman J., Linstead P., Boudonck K., Steer M.,
RA Shaw P., Dolan L.;
RT "KOJAK encodes a cellulose synthase-like protein required for root hair
RT cell morphogenesis in Arabidopsis.";
RL Genes Dev. 15:79-89(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND TISSUE SPECIFICITY.
RC STRAIN=cv. Columbia;
RX PubMed=11402188; DOI=10.1104/pp.126.2.575;
RA Wang X., Cnops G., Vanderhaeghen R., de Block S., van Montagu M.,
RA van Lijsebettens M.;
RT "AtCSLD3, a cellulose synthase-like gene important for root hair growth in
RT arabidopsis.";
RL Plant Physiol. 126:575-586(2001).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130713; DOI=10.1038/35048706;
RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL Nature 408:820-822(2000).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [6]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=11027699; DOI=10.1104/pp.124.2.495;
RA Richmond T.A., Somerville C.R.;
RT "The cellulose synthase superfamily.";
RL Plant Physiol. 124:495-498(2000).
RN [7]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-755, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC STRAIN=cv. La-0;
RX PubMed=14506206; DOI=10.1074/mcp.t300006-mcp200;
RA Nuehse T.S., Stensballe A., Jensen O.N., Peck S.C.;
RT "Large-scale analysis of in vivo phosphorylated membrane proteins by
RT immobilized metal ion affinity chromatography and mass spectrometry.";
RL Mol. Cell. Proteomics 2:1234-1243(2003).
RN [8]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-755, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=15308754; DOI=10.1105/tpc.104.023150;
RA Nuehse T.S., Stensballe A., Jensen O.N., Peck S.C.;
RT "Phosphoproteomics of the Arabidopsis plasma membrane and a new
RT phosphorylation site database.";
RL Plant Cell 16:2394-2405(2004).
RN [9]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-755, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC STRAIN=cv. Columbia;
RX PubMed=19245862; DOI=10.1016/j.jprot.2009.02.004;
RA Jones A.M.E., MacLean D., Studholme D.J., Serna-Sanz A., Andreasson E.,
RA Rathjen J.P., Peck S.C.;
RT "Phosphoproteomic analysis of nuclei-enriched fractions from Arabidopsis
RT thaliana.";
RL J. Proteomics 72:439-451(2009).
RN [10]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19376835; DOI=10.1104/pp.109.138677;
RA Reiland S., Messerli G., Baerenfaller K., Gerrits B., Endler A.,
RA Grossmann J., Gruissem W., Baginsky S.;
RT "Large-scale Arabidopsis phosphoproteome profiling reveals novel
RT chloroplast kinase substrates and phosphorylation networks.";
RL Plant Physiol. 150:889-903(2009).
CC -!- FUNCTION: Thought to be a Golgi-localized beta-glycan synthase that
CC polymerize the backbones of noncellulosic polysaccharides
CC (hemicelluloses) of plant cell wall. Required for synthesis of a cell
CC wall polysaccharide essential for root hair elongation, but not
CC initiation. May be the functional ortholog of rice CSLD1.
CC {ECO:0000269|PubMed:11402188}.
CC -!- SUBCELLULAR LOCATION: Golgi apparatus membrane
CC {ECO:0000305|PubMed:11156607}; Multi-pass membrane protein
CC {ECO:0000305|PubMed:11156607}.
CC -!- TISSUE SPECIFICITY: Preferentially expressed in root hair cells.
CC Expressed in roots, leaves, stems, flowers and siliques.
CC {ECO:0000269|PubMed:11156607, ECO:0000269|PubMed:11402188}.
CC -!- SIMILARITY: Belongs to the glycosyltransferase 2 family. Plant
CC cellulose synthase-like D subfamily. {ECO:0000305}.
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DR EMBL; AF232907; AAG60543.1; -; mRNA.
DR EMBL; AJ297948; CAC82909.1; -; Genomic_DNA.
DR EMBL; AC012328; AAF26119.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE73896.1; -; Genomic_DNA.
DR EMBL; AF360180; AAK25890.1; -; mRNA.
DR EMBL; AY039996; AAK64073.1; -; mRNA.
DR RefSeq; NP_186955.1; NM_111175.3.
DR AlphaFoldDB; Q9M9M4; -.
DR SMR; Q9M9M4; -.
DR BioGRID; 6481; 2.
DR IntAct; Q9M9M4; 2.
DR STRING; 3702.AT3G03050.1; -.
DR CAZy; GT2; Glycosyltransferase Family 2.
DR iPTMnet; Q9M9M4; -.
DR PaxDb; Q9M9M4; -.
DR PRIDE; Q9M9M4; -.
DR ProteomicsDB; 220366; -.
DR EnsemblPlants; AT3G03050.1; AT3G03050.1; AT3G03050.
DR GeneID; 821148; -.
DR Gramene; AT3G03050.1; AT3G03050.1; AT3G03050.
DR KEGG; ath:AT3G03050; -.
DR Araport; AT3G03050; -.
DR TAIR; locus:2097700; AT3G03050.
DR eggNOG; ENOG502QU14; Eukaryota.
DR HOGENOM; CLU_001418_0_2_1; -.
DR InParanoid; Q9M9M4; -.
DR OMA; MWRIKHK; -.
DR OrthoDB; 679241at2759; -.
DR PhylomeDB; Q9M9M4; -.
DR BioCyc; ARA:AT3G03050-MON; -.
DR BRENDA; 2.4.1.12; 399.
DR PRO; PR:Q9M9M4; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q9M9M4; baseline and differential.
DR Genevisible; Q9M9M4; AT.
DR GO; GO:0005783; C:endoplasmic reticulum; IDA:TAIR.
DR GO; GO:0005768; C:endosome; HDA:TAIR.
DR GO; GO:0005794; C:Golgi apparatus; HDA:TAIR.
DR GO; GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; HDA:TAIR.
DR GO; GO:0005802; C:trans-Golgi network; HDA:TAIR.
DR GO; GO:0016760; F:cellulose synthase (UDP-forming) activity; IEA:InterPro.
DR GO; GO:0051753; F:mannan synthase activity; IDA:TAIR.
DR GO; GO:0071555; P:cell wall organization; IEA:UniProtKB-KW.
DR GO; GO:0030244; P:cellulose biosynthetic process; IEA:InterPro.
DR GO; GO:0009833; P:plant-type primary cell wall biogenesis; IBA:GO_Central.
DR GO; GO:0009409; P:response to cold; IEP:TAIR.
DR Gene3D; 3.30.40.10; -; 1.
DR Gene3D; 3.90.550.10; -; 1.
DR InterPro; IPR005150; Cellulose_synth.
DR InterPro; IPR029044; Nucleotide-diphossugar_trans.
DR InterPro; IPR013083; Znf_RING/FYVE/PHD.
DR Pfam; PF03552; Cellulose_synt; 1.
PE 1: Evidence at protein level;
KW Cell wall biogenesis/degradation; Glycosyltransferase; Golgi apparatus;
KW Membrane; Phosphoprotein; Reference proteome; Transferase; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..1145
FT /note="Cellulose synthase-like protein D3"
FT /id="PRO_0000319348"
FT TRANSMEM 289..309
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 319..339
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 930..950
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 956..976
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 1002..1022
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 1045..1065
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 1079..1099
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 1109..1129
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 1..38
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 189..208
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 419
FT /evidence="ECO:0000255"
FT ACT_SITE 848
FT /evidence="ECO:0000255"
FT MOD_RES 755
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:14506206,
FT ECO:0007744|PubMed:15308754, ECO:0007744|PubMed:19245862"
FT MUTAGEN 829
FT /note="E->K: In kjk-3; reduction of root hair growth."
FT /evidence="ECO:0000269|PubMed:11156607"
SQ SEQUENCE 1145 AA; 128589 MW; F5763EF66124E33D CRC64;
MASNNHFMNS RSNLSTNSDA AEAERHQQPV SNSVTFARRT PSGRYVNYSR DDLDSELGSV
DLTGYSVHIP PTPDNQPMDP SISQKVEEQY VSNSLFTGGF NSVTRAHLME KVIDTETSHP
QMAGAKGSSC AVPGCDVKVM SDERGQDLLP CECDFKICRD CFMDAVKTGG MCPGCKEPYR
NTDLADFADN NKQQRPMLPP PAGGSKMDRR LSLMKSTKSG LMRSQTGDFD HNRWLFETSG
TYGFGNAFWT KDGNFGSDKD GNGHGMGPQD LMSRPWRPLT RKLQIPAAVI SPYRLLILIR
IVVLALFLMW RIKHKNPDAI WLWGMSVVCE LWFALSWLLD QLPKLCPINR ATDLNVLKEK
FETPTPSNPT GKSDLPGLDM FVSTADPEKE PPLVTSNTIL SILAADYPVE KLACYVSDDG
GALLTFEAMA EAASFANMWV PFCRKHNIEP RNPDSYFSLK RDPYKNKVKA DFVKDRRRVK
REYDEFKVRI NSLPDSIRRR SDAYHAREEI KAMKLQRQNR DEEIVEPVKI PKATWMADGT
HWPGTWINSG PDHSRSDHAG IIQVMLKPPS DEPLHGVSEG FLDLTDVDIR LPLLVYVSRE
KRPGYDHNKK AGAMNALVRA SAIMSNGPFI LNLDCDHYIY NSQALREGMC FMMDRGGDRL
CYVQFPQRFE GIDPSDRYAN HNTVFFDVNM RALDGLMGPV YVGTGCLFRR IALYGFDPPR
AKEHHPGFCS CCFSRKKKKS RVPEENRSLR MGGDSDDDEE MNLSLVPKKF GNSTFLIDSI
PVAEFQGRPL ADHPAVQNGR PPGALTIPRE LLDASTVAEA IAVISCWYED KTEWGSRIGW
IYGSVTEDVV TGYRMHNRGW KSVYCVTKRD AFRGTAPINL TDRLHQVLRW ATGSVEIFFS
RNNAFFASPR MKILQRIAYL NVGIYPFTSF FLIVYCFLPA LSLFSGQFIV QTLNVTFLVY
LLIISITLCL LALLEIKWSG ISLEEWWRNE QFWLIGGTSA HLAAVIQGLL KVVAGIEISF
TLTSKSGGED VDDEFADLYI VKWTSLMIPP ITIMMVNLIA IAVGFSRTIY SVIPQWSKLI
GGVFFSFWVL AHLYPFAKGL MGRRGRTPTI VYVWSGLVAI TISLLWVAIN PPAGSTQIGG
SFTFP
