CSLF2_ORYSJ
ID CSLF2_ORYSJ Reviewed; 889 AA.
AC Q84S11; A0A0P0X7S7; Q8W1M8; Q944E1;
DT 26-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2003, sequence version 1.
DT 03-AUG-2022, entry version 103.
DE RecName: Full=Mixed-linked glucan synthase 2;
DE EC=2.4.1.-;
DE AltName: Full=1,3;1,4-beta-D-glucan synthase 2;
DE AltName: Full=Cellulose synthase-like protein F2;
DE AltName: Full=OsCslF2;
GN Name=CSLF2; OrderedLocusNames=Os07g0552800, LOC_Os07g36690;
GN ORFNames=OSJNBb0041B22.126, P0013G11.2;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND NUCLEOTIDE SEQUENCE [MRNA] OF
RP 434-889.
RX PubMed=11842136; DOI=10.1104/pp.010875;
RA Hazen S.P., Scott-Craig J.S., Walton J.D.;
RT "Cellulose synthase-like genes of rice.";
RL Plant Physiol. 128:336-340(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
RN [6]
RP FUNCTION.
RX PubMed=16574868; DOI=10.1126/science.1122975;
RA Burton R.A., Wilson S.M., Hrmova M., Harvey A.J., Shirley N.J.,
RA Medhurst A., Stone B.A., Newbigin E.J., Bacic A., Fincher G.B.;
RT "Cellulose synthase-like CslF genes mediate the synthesis of cell wall
RT (1,3;1,4)-beta-D-glucans.";
RL Science 311:1940-1942(2006).
CC -!- FUNCTION: Catalyzes both beta-1,3 and beta-1,4 glycosidic linkage on
CC beta-D-glucan. Essential for (1,3;1,4)-beta-D-glucans synthesis in
CC grasses and cereals (Poaceae). The mixed-linked glucans (which are not
CC present in walls of dicotyledons or most other monocotyledonous plants)
CC are particularly important constituents of the walls of the starchy
CC endosperm and aleurone cells of cereal grains such as oats, wheat, rice
CC and barley. They can account for up to 70% by weight of the wall.
CC {ECO:0000269|PubMed:16574868}.
CC -!- SUBCELLULAR LOCATION: Golgi apparatus membrane {ECO:0000305}; Multi-
CC pass membrane protein {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the glycosyltransferase 2 family. Plant
CC cellulose synthase-like F subfamily. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AK100523; Type=Erroneous termination; Note=Truncated C-terminus.; Evidence={ECO:0000305};
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DR EMBL; AF432503; AAL25132.1; -; Genomic_DNA.
DR EMBL; AF435651; AAL38536.1; -; mRNA.
DR EMBL; AP004261; BAD30521.1; -; Genomic_DNA.
DR EMBL; AP005126; BAC65378.1; -; Genomic_DNA.
DR EMBL; AP008213; BAF21860.1; -; Genomic_DNA.
DR EMBL; AP014963; BAT02064.1; -; Genomic_DNA.
DR EMBL; AK100523; -; NOT_ANNOTATED_CDS; mRNA.
DR RefSeq; XP_015645336.1; XM_015789850.1.
DR AlphaFoldDB; Q84S11; -.
DR SMR; Q84S11; -.
DR STRING; 4530.OS07T0552800-01; -.
DR CAZy; GT2; Glycosyltransferase Family 2.
DR PaxDb; Q84S11; -.
DR PRIDE; Q84S11; -.
DR EnsemblPlants; Os07t0552800-01; Os07t0552800-01; Os07g0552800.
DR GeneID; 4343553; -.
DR Gramene; Os07t0552800-01; Os07t0552800-01; Os07g0552800.
DR KEGG; osa:4343553; -.
DR eggNOG; ENOG502QU14; Eukaryota.
DR InParanoid; Q84S11; -.
DR OrthoDB; 679241at2759; -.
DR Proteomes; UP000000763; Chromosome 7.
DR Proteomes; UP000059680; Chromosome 7.
DR ExpressionAtlas; Q84S11; baseline and differential.
DR Genevisible; Q84S11; OS.
DR GO; GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0016760; F:cellulose synthase (UDP-forming) activity; IEA:InterPro.
DR GO; GO:0051753; F:mannan synthase activity; IBA:GO_Central.
DR GO; GO:0071555; P:cell wall organization; IEA:UniProtKB-KW.
DR GO; GO:0030244; P:cellulose biosynthetic process; IEA:InterPro.
DR GO; GO:0009833; P:plant-type primary cell wall biogenesis; IBA:GO_Central.
DR Gene3D; 3.90.550.10; -; 1.
DR InterPro; IPR005150; Cellulose_synth.
DR InterPro; IPR029044; Nucleotide-diphossugar_trans.
DR Pfam; PF03552; Cellulose_synt; 2.
DR SUPFAM; SSF53448; SSF53448; 1.
PE 2: Evidence at transcript level;
KW Cell wall biogenesis/degradation; Coiled coil; Glycosyltransferase;
KW Golgi apparatus; Membrane; Reference proteome; Transferase; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..889
FT /note="Mixed-linked glucan synthase 2"
FT /id="PRO_0000319402"
FT TRANSMEM 93..113
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 123..143
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 655..675
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 685..705
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 723..743
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 777..797
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 811..831
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 842..862
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 34..53
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 265..293
FT /evidence="ECO:0000255"
FT ACT_SITE 213
FT /evidence="ECO:0000255"
FT ACT_SITE 579
FT /evidence="ECO:0000255"
FT BINDING 411
FT /ligand="substrate"
FT /evidence="ECO:0000255"
FT BINDING 413
FT /ligand="substrate"
FT /evidence="ECO:0000255"
SQ SEQUENCE 889 AA; 97771 MW; CBD5D1FDABAF7656 CRC64;
MAATAASTMS AAAAVTRRIN AALRVDATSG DVAAGADGQN GRRSPVAKRV NDGGGGKDDV
WVAVDEKDVC GARGGDGAAR PPLFRTYKVK GSILHPYRFL ILLRLIAIVA FFAWRVRHKN
RDGVWLWTMS MVGDVWFGFS WVLNQLPKLS PIKRVPDLAA LADRHSGDLP GVDVFVTTVD
PVDEPILYTV NTILSILAAD YPVDRYACYL SDDGGTLVHY EAMVEVAKFA ELWVPFCRKH
CVEPRSPENY FAMKTQAYKG GVPGELMSDH RRVRREYEEF KVRIDSLSST IRQRSDVYNA
KHAGENATWM ADGTHWPGTW FEPADNHQRG KHAGIVQVLL NHPSCKPRLG LAASAENPVD
FSGVDVRLPM LVYISREKRP GYNHQKKAGA MNVMLRVSAL LSNAPFVINF DGDHYVNNSQ
AFRAPMCFML DGRGRGGENT AFVQFPQRFD DVDPTDRYAN HNRVFFDGTM LSLNGLQGPS
YLGTGTMFRR VALYGVEPPR WGAAASQIKA MDIANKFGSS TSFVGTMLDG ANQERSITPL
AVLDESVAGD LAALTACAYE DGTSWGRDVG WVYNIATEDV VTGFRMHRQG WRSVYASVEP
AAFRGTAPIN LTERLYQILR WSGGSLEMFF SHSNALLAGR RLHPLQRVAY LNMSTYPIVT
VFIFFYNLFP VMWLISEQYY IQRPFGEYLL YLVAVIAMIH VIGMFEVKWA GITLLDWCRN
EQFYMIGSTG VYPTAVLYMA LKLVTGKGIY FRLTSKQTTA SSGDKFADLY TVRWVPLLIP
TIVIIVVNVA AVGVAVGKAA AWGPLTEPGW LAVLGMVFNV WILVLLYPFA LGVMGQWGKR
PAVLFVAMAM AVAAVAAMYV AFGAPYQAEL SGGAASLGKA AASLTGPSG
