CSLF4_ORYSJ
ID CSLF4_ORYSJ Reviewed; 897 AA.
AC Q6ZF86; Q944D9;
DT 26-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 90.
DE RecName: Full=Mixed-linked glucan synthase 4;
DE EC=2.4.1.-;
DE AltName: Full=1,3;1,4-beta-D-glucan synthase 4;
DE AltName: Full=Cellulose synthase-like protein F4;
DE AltName: Full=OsCslF4;
GN Name=CSLF4; OrderedLocusNames=Os07g0553300, LOC_Os07g36740;
GN ORFNames=P0013G11.11;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=11842136; DOI=10.1104/pp.010875;
RA Hazen S.P., Scott-Craig J.S., Walton J.D.;
RT "Cellulose synthase-like genes of rice.";
RL Plant Physiol. 128:336-340(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [5]
RP FUNCTION.
RX PubMed=16574868; DOI=10.1126/science.1122975;
RA Burton R.A., Wilson S.M., Hrmova M., Harvey A.J., Shirley N.J.,
RA Medhurst A., Stone B.A., Newbigin E.J., Bacic A., Fincher G.B.;
RT "Cellulose synthase-like CslF genes mediate the synthesis of cell wall
RT (1,3;1,4)-beta-D-glucans.";
RL Science 311:1940-1942(2006).
CC -!- FUNCTION: Catalyzes both beta-1,3 and beta-1,4 glycosidic linkage on
CC beta-D-glucan. Essential for (1,3;1,4)-beta-D-glucans synthesis in
CC grasses and cereals (Poaceae). The mixed-linked glucans (which are not
CC present in walls of dicotyledons or most other monocotyledonous plants)
CC are particularly important constituents of the walls of the starchy
CC endosperm and aleurone cells of cereal grains such as oats, wheat, rice
CC and barley. They can account for up to 70% by weight of the wall.
CC {ECO:0000269|PubMed:16574868}.
CC -!- SUBCELLULAR LOCATION: Golgi apparatus membrane {ECO:0000305}; Multi-
CC pass membrane protein {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the glycosyltransferase 2 family. Plant
CC cellulose synthase-like F subfamily. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAL25134.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AF432505; AAL25134.1; ALT_INIT; Genomic_DNA.
DR EMBL; AP004261; BAC83321.1; -; Genomic_DNA.
DR EMBL; AP008213; BAF21861.1; -; Genomic_DNA.
DR EMBL; AP014963; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR RefSeq; XP_015645689.1; XM_015790203.1.
DR AlphaFoldDB; Q6ZF86; -.
DR SMR; Q6ZF86; -.
DR CAZy; GT2; Glycosyltransferase Family 2.
DR PaxDb; Q6ZF86; -.
DR PRIDE; Q6ZF86; -.
DR GeneID; 4343554; -.
DR KEGG; osa:4343554; -.
DR InParanoid; Q6ZF86; -.
DR OrthoDB; 679241at2759; -.
DR Proteomes; UP000000763; Chromosome 7.
DR Proteomes; UP000059680; Chromosome 7.
DR GO; GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0016760; F:cellulose synthase (UDP-forming) activity; IEA:InterPro.
DR GO; GO:0051753; F:mannan synthase activity; IBA:GO_Central.
DR GO; GO:0071555; P:cell wall organization; IEA:UniProtKB-KW.
DR GO; GO:0030244; P:cellulose biosynthetic process; IEA:InterPro.
DR GO; GO:0009833; P:plant-type primary cell wall biogenesis; IBA:GO_Central.
DR Gene3D; 3.90.550.10; -; 1.
DR InterPro; IPR005150; Cellulose_synth.
DR InterPro; IPR029044; Nucleotide-diphossugar_trans.
DR Pfam; PF03552; Cellulose_synt; 2.
DR SUPFAM; SSF53448; SSF53448; 1.
PE 3: Inferred from homology;
KW Cell wall biogenesis/degradation; Glycosyltransferase; Golgi apparatus;
KW Membrane; Reference proteome; Transferase; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..897
FT /note="Mixed-linked glucan synthase 4"
FT /id="PRO_0000319405"
FT TRANSMEM 93..113
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 123..143
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 664..684
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 694..714
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 732..752
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 786..806
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 822..842
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 851..871
FT /note="Helical"
FT /evidence="ECO:0000255"
FT ACT_SITE 219
FT /evidence="ECO:0000255"
FT ACT_SITE 588
FT /evidence="ECO:0000255"
FT BINDING 421
FT /ligand="substrate"
FT /evidence="ECO:0000255"
FT BINDING 423
FT /ligand="substrate"
FT /evidence="ECO:0000255"
SQ SEQUENCE 897 AA; 98353 MW; 4CD2BE82ADF2AF6E CRC64;
MELATASTMS AAAVTRRINA GGLRVEVTNG NGAAGVYVAA AAAPCSPAAK RVNDGGGKDD
VWVAVDEADV SGPSGGDGVR PTLFRTYKVK GSILHPYRFL ILVRLIAIVA FFAWRVRHKN
RDGAWLWTMS MAGDVWFGFS WALNQLPKLN PIKRVADLAA LADRQQHGTS GGGELPGVDV
FVTTVDPVDE PILYTVNSIL SILAADYPVD RYACYLSDDG GTLVHYEAMV EVAKFAELWV
PFCRKHCVEP RAPESYFAMK TQAYRGGVAG ELMSDRRRVR REYEEFKVRI DSLFSTIRKR
SDAYNRAKDG KDDGENATWM ADGTHWPGTW FEPAENHRKG QHAGIVQVLL NHPTSKPRFG
VAASVDNPLD FSGVDVRLPM LVYISREKRP GYNHQKKAGA MNALLRVSAL LSNAPFIINF
DCDHYVNNSQ AFRAPMCFML DRRGGGDDVA FVQFPQRFDD VDPTDRYANH NRVFFDGTTL
SLNGLQGPSY LGTGTMFRRA ALYGLEPPRW GAAGSQIKAM DNANKFGASS TLVSSMLDGA
NQERSITPPV AIDGSVARDL AAVTACGYDL GTSWGRDAGW VYDIATEDVA TGFRMHQQGW
RSVYTSMEPA AFRGTAPINL TERLYQILRW SGGSLEMFFS HSNALLAGRR LHPLQRIAYL
NMSTYPIVTV FIFFYNLFPV MWLISEQYYI QQPFGEYLLY LVAIIAMIHV IGMFEVKWSG
ITVLDWCRNE QFYMIGSTGV YPTAVLYMAL KLFTGKGIHF RLTSKQTTAS SGDKFADLYT
VRWVPLLIPT IVVLAVNVGA VGVAVGKAAA WGLLTEQGRF AVLGMVFNVW ILALLYPFAL
GIMGQRGKRP AVLFVATVMA VAAVAIMYAA FGAPYQAGLS GVAASLGKAA SLTGPSG
