ID   CSLF6_ORYSJ             Reviewed;         952 AA.
AC   Q84UP7; A0A0N7KPB2; Q8W1N4;
DT   26-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2003, sequence version 1.
DT   03-AUG-2022, entry version 100.
DE   RecName: Full=Probable mixed-linked glucan synthase 6;
DE            EC=2.4.1.-;
DE   AltName: Full=1,3;1,4-beta-D-glucan synthase 6;
DE   AltName: Full=Cellulose synthase-like protein F6;
DE   AltName: Full=OsCslF6;
GN   Name=CSLF6; OrderedLocusNames=Os08g0160500, LOC_Os08g06380;
GN   ORFNames=OsJ_025078, P0577B11.104-1, P0672D01.120-1;
OS   Oryza sativa subsp. japonica (Rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX   NCBI_TaxID=39947;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16100779; DOI=10.1038/nature03895;
RG   International rice genome sequencing project (IRGSP);
RT   "The map-based sequence of the rice genome.";
RL   Nature 436:793-800(2005).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=18089549; DOI=10.1093/nar/gkm978;
RG   The rice annotation project (RAP);
RT   "The rice annotation project database (RAP-DB): 2008 update.";
RL   Nucleic Acids Res. 36:D1028-D1033(2008).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA   Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA   Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA   Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA   Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT   "Improvement of the Oryza sativa Nipponbare reference genome using next
RT   generation sequence and optical map data.";
RL   Rice 6:4-4(2013).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA   Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA   Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA   Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA   Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA   Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA   Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA   Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA   Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA   Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA   Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA   Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA   Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA   Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA   McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT   "The genomes of Oryza sativa: a history of duplications.";
RL   PLoS Biol. 3:266-281(2005).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=12869764; DOI=10.1126/science.1081288;
RG   The rice full-length cDNA consortium;
RT   "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT   japonica rice.";
RL   Science 301:376-379(2003).
RN   [6]
RP   NUCLEOTIDE SEQUENCE [MRNA] OF 436-952.
RX   PubMed=11842136; DOI=10.1104/pp.010875;
RA   Hazen S.P., Scott-Craig J.S., Walton J.D.;
RT   "Cellulose synthase-like genes of rice.";
RL   Plant Physiol. 128:336-340(2002).
CC   -!- FUNCTION: May catalyze both beta-1,3 and beta-1,4 glycosidic linkage on
CC       beta-D-glucan. Essential for (1,3;1,4)-beta-D-glucans synthesis in
CC       grasses and cereals (Poaceae). The mixed-linked glucans (which are not
CC       present in walls of dicotyledons or most other monocotyledonous plants)
CC       are particularly important constituents of the walls of the starchy
CC       endosperm and aleurone cells of cereal grains such as oats, wheat, rice
CC       and barley. They can account for up to 70% by weight of the wall (By
CC       similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Golgi apparatus membrane {ECO:0000305}; Multi-
CC       pass membrane protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the glycosyltransferase 2 family. Plant
CC       cellulose synthase-like F subfamily. {ECO:0000305}.
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DR   EMBL; AP004635; BAC66734.1; -; Genomic_DNA.
DR   EMBL; AP005504; BAC99779.1; -; Genomic_DNA.
DR   EMBL; AP008214; BAF22962.1; -; Genomic_DNA.
DR   EMBL; AP014964; BAT03930.1; -; Genomic_DNA.
DR   EMBL; CM000145; EAZ41595.1; -; Genomic_DNA.
DR   EMBL; AK065259; -; NOT_ANNOTATED_CDS; mRNA.
DR   EMBL; AF435645; AAL38530.2; -; mRNA.
DR   RefSeq; XP_015650332.1; XM_015794846.1.
DR   AlphaFoldDB; Q84UP7; -.
DR   SMR; Q84UP7; -.
DR   STRING; 4530.OS08T0160500-01; -.
DR   CAZy; GT2; Glycosyltransferase Family 2.
DR   TCDB; 4.D.3.1.8; the glycan glucosyl transferase (opgh) family.
DR   PaxDb; Q84UP7; -.
DR   PRIDE; Q84UP7; -.
DR   EnsemblPlants; Os08t0160500-01; Os08t0160500-01; Os08g0160500.
DR   GeneID; 4344719; -.
DR   Gramene; Os08t0160500-01; Os08t0160500-01; Os08g0160500.
DR   KEGG; osa:4344719; -.
DR   eggNOG; ENOG502QU14; Eukaryota.
DR   HOGENOM; CLU_001418_3_1_1; -.
DR   InParanoid; Q84UP7; -.
DR   OMA; VCTAAYE; -.
DR   OrthoDB; 679241at2759; -.
DR   Proteomes; UP000000763; Chromosome 8.
DR   Proteomes; UP000007752; Chromosome 8.
DR   Proteomes; UP000059680; Chromosome 8.
DR   ExpressionAtlas; Q84UP7; baseline and differential.
DR   Genevisible; Q84UP7; OS.
DR   GO; GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR   GO; GO:0016760; F:cellulose synthase (UDP-forming) activity; IEA:InterPro.
DR   GO; GO:0051753; F:mannan synthase activity; IBA:GO_Central.
DR   GO; GO:0071555; P:cell wall organization; IEA:UniProtKB-KW.
DR   GO; GO:0030244; P:cellulose biosynthetic process; IEA:InterPro.
DR   GO; GO:0009833; P:plant-type primary cell wall biogenesis; IBA:GO_Central.
DR   Gene3D; 3.90.550.10; -; 1.
DR   InterPro; IPR005150; Cellulose_synth.
DR   InterPro; IPR029044; Nucleotide-diphossugar_trans.
DR   Pfam; PF03552; Cellulose_synt; 1.
DR   SUPFAM; SSF53448; SSF53448; 1.
PE   2: Evidence at transcript level;
KW   Cell wall biogenesis/degradation; Coiled coil; Glycosyltransferase;
KW   Golgi apparatus; Membrane; Reference proteome; Transferase; Transmembrane;
KW   Transmembrane helix.
FT   CHAIN           1..952
FT                   /note="Probable mixed-linked glucan synthase 6"
FT                   /id="PRO_0000319406"
FT   TRANSMEM        102..122
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        132..152
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        718..738
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        744..764
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        782..802
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        834..854
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        865..885
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        898..918
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   COILED          278..308
FT                   /evidence="ECO:0000255"
FT   ACT_SITE        227
FT                   /evidence="ECO:0000255"
FT   ACT_SITE        636
FT                   /evidence="ECO:0000255"
FT   BINDING         429
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000255"
FT   BINDING         431
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000255"
FT   CONFLICT        912
FT                   /note="A -> V (in Ref. 5; AK065259)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   952 AA;  105152 MW;  4DF4D3A364998DBC CRC64;
     MAPAVAGGGG RRNNEGVNGN AAAPACVCGF PVCACAGAAA VASAASSADM DIVAAGQIGA
     VNDESWVAVD LSDSDDAPAA GDVQGALDDR PVFRTEKIKG VLLHPYRVLI FVRLIAFTLF
     VIWRIEHKNP DAMWLWVTSI AGEFWFGFSW LLDQLPKLNP INRVPDLAVL RRRFDHADGT
     SSLPGLDIFV TTADPIKEPI LSTANSILSI LAADYPVDRN TCYLSDDSGM LLTYEAMAEA
     AKFATLWVPF CRKHAIEPRG PESYFELKSH PYMGRAQEEF VNDRRRVRKE YDDFKARING
     LEHDIKQRSD SYNAAAGVKD GEPRATWMAD GSQWEGTWIE QSENHRKGDH AGIVLVLLNH
     PSHARQLGPP ASADNPLDFS GVDVRLPMLV YVAREKRPGC NHQKKAGAMN ALTRASAVLS
     NSPFILNLDC DHYINNSQAL RAGICFMLGR DSDTVAFVQF PQRFEGVDPT DLYANHNRIF
     FDGTLRALDG LQGPIYVGTG CLFRRITLYG FEPPRINVGG PCFPRLGGMF AKNRYQKPGF
     EMTKPGAKPV APPPAATVAK GKHGFLPMPK KAYGKSDAFA DTIPRASHPS PYAAEAAVAA
     DEAAIAEAVM VTAAAYEKKT GWGSDIGWVY GTVTEDVVTG YRMHIKGWRS RYCSIYPHAF
     IGTAPINLTE RLFQVLRWST GSLEIFFSRN NPLFGSTFLH PLQRVAYINI TTYPFTALFL
     IFYTTVPALS FVTGHFIVQR PTTMFYVYLA IVLGTLLILA VLEVKWAGVT VFEWFRNGQF
     WMTASCSAYL AAVLQVVTKV VFRRDISFKL TSKLPAGDEK KDPYADLYVV RWTWLMITPI
     IIILVNIIGS AVAFAKVLDG EWTHWLKVAG GVFFNFWVLF HLYPFAKGIL GKHGKTPVVV
     LVWWAFTFVI TAVLYINIPH IHGPGRHGAA SPSHGHHSAH GTKKYDFTYA WP