CSLF8_ORYSJ
ID CSLF8_ORYSJ Reviewed; 886 AA.
AC Q84S18; A0A0P0X7A2;
DT 26-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2003, sequence version 1.
DT 03-AUG-2022, entry version 100.
DE RecName: Full=Probable mixed-linked glucan synthase 8;
DE EC=2.4.1.-;
DE AltName: Full=1,3;1,4-beta-D-glucan synthase 8;
DE AltName: Full=Cellulose synthase-like protein F8;
DE AltName: Full=OsCslF8;
GN Name=CSFL8; OrderedLocusNames=Os07g0551700, LOC_Os07g36630;
GN ORFNames=OSJNBb0041B22.114;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
RN [5]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=11842136; DOI=10.1104/pp.010875;
RA Hazen S.P., Scott-Craig J.S., Walton J.D.;
RT "Cellulose synthase-like genes of rice.";
RL Plant Physiol. 128:336-340(2002).
CC -!- FUNCTION: May catalyze both beta-1,3 and beta-1,4 glycosidic linkage on
CC beta-D-glucan. Essential for (1,3;1,4)-beta-D-glucans synthesis in
CC grasses and cereals (Poaceae). The mixed-linked glucans (which are not
CC present in walls of dicotyledons or most other monocotyledonous plants)
CC are particularly important constituents of the walls of the starchy
CC endosperm and aleurone cells of cereal grains such as oats, wheat, rice
CC and barley. They can account for up to 70% by weight of the wall (By
CC similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Golgi apparatus membrane {ECO:0000305}; Multi-
CC pass membrane protein {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the glycosyltransferase 2 family. Plant
CC cellulose synthase-like F subfamily. {ECO:0000305}.
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DR EMBL; AP005126; BAC65371.1; -; Genomic_DNA.
DR EMBL; AP008213; BAF21859.1; -; Genomic_DNA.
DR EMBL; AP014963; BAT02056.1; -; Genomic_DNA.
DR EMBL; AK067424; -; NOT_ANNOTATED_CDS; mRNA.
DR RefSeq; XP_015645599.1; XM_015790113.1.
DR AlphaFoldDB; Q84S18; -.
DR SMR; Q84S18; -.
DR STRING; 4530.OS07T0551700-01; -.
DR CAZy; GT2; Glycosyltransferase Family 2.
DR PaxDb; Q84S18; -.
DR PRIDE; Q84S18; -.
DR EnsemblPlants; Os07t0551700-01; Os07t0551700-01; Os07g0551700.
DR GeneID; 4343552; -.
DR Gramene; Os07t0551700-01; Os07t0551700-01; Os07g0551700.
DR KEGG; osa:4343552; -.
DR eggNOG; ENOG502QU14; Eukaryota.
DR HOGENOM; CLU_001418_3_1_1; -.
DR InParanoid; Q84S18; -.
DR OMA; KCAYEEG; -.
DR OrthoDB; 679241at2759; -.
DR Proteomes; UP000000763; Chromosome 7.
DR Proteomes; UP000059680; Chromosome 7.
DR Genevisible; Q84S18; OS.
DR GO; GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0016760; F:cellulose synthase (UDP-forming) activity; IEA:InterPro.
DR GO; GO:0051753; F:mannan synthase activity; IBA:GO_Central.
DR GO; GO:0071555; P:cell wall organization; IEA:UniProtKB-KW.
DR GO; GO:0030244; P:cellulose biosynthetic process; IEA:InterPro.
DR GO; GO:0009833; P:plant-type primary cell wall biogenesis; IBA:GO_Central.
DR Gene3D; 3.90.550.10; -; 1.
DR InterPro; IPR005150; Cellulose_synth.
DR InterPro; IPR029044; Nucleotide-diphossugar_trans.
DR Pfam; PF03552; Cellulose_synt; 2.
DR SUPFAM; SSF53448; SSF53448; 1.
PE 2: Evidence at transcript level;
KW Cell wall biogenesis/degradation; Glycosyltransferase; Golgi apparatus;
KW Membrane; Reference proteome; Transferase; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..886
FT /note="Probable mixed-linked glucan synthase 8"
FT /id="PRO_0000319408"
FT TRANSMEM 87..107
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 118..138
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 659..679
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 683..703
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 723..743
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 775..795
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 812..832
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 840..860
FT /note="Helical"
FT /evidence="ECO:0000255"
FT ACT_SITE 213
FT /evidence="ECO:0000255"
FT ACT_SITE 577
FT /evidence="ECO:0000255"
FT BINDING 413
FT /ligand="substrate"
FT /evidence="ECO:0000255"
FT BINDING 415
FT /ligand="substrate"
FT /evidence="ECO:0000255"
FT CONFLICT 720
FT /note="Q -> L (in Ref. 4; AK067424)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 886 AA; 98774 MW; 20CDD5BDD31E913B CRC64;
MAANGGGGGA GGCSNGGGGG AVNGAAANGG GGGGGGSKGA TTRRAKVSPM DRYWVPTDEK
EMAAAVADGG EDGRRPLLFR TFTVRGILLH PYRLLTLVRL VAIVLFFIWR IRHPYADGMF
FWWISVIGDF WFGVSWLLNQ VAKLKPIRRV PDLNLLQQQF DLPDGNSNLP GLDVFINTVD
PINEPMIYTM NAILSILAAD YPVDKHACYL SDDGGSIIHY DGLLETAKFA ALWVPFCRKH
SIEPRAPESY FAVKSRPYAG SAPEDFLSDH RYMRREYDEF KVRLDALFTV IPKRSDAYNQ
AHAEEGVKAT WMADGTEWPG TWIDPSENHK KGNHAGIVQV MLNHPSNQPQ LGLPASTDSP
VDFSNVDVRL PMLVYIAREK RPGYDHQKKA GAMNVQLRVS ALLTNAPFII NFDGDHYVNN
SKAFRAGICF MLDRREGDNT AFVQFPQRFD DVDPTDRYCN HNRVFFDATL LGLNGIQGPS
YVGTGCMFRR VALYGVDPPR WRPDDGNIVD SSKKFGNLDS FISSIPIAAN QERSIISPPA
LEESILQELS DAMACAYEDG TDWGKDVGWV YNIATEDVVT GFRLHRTGWR SMYCRMEPDA
FRGTAPINLT ERLYQILRWS GGSLEMFFSH NCPLLAGRRL NFMQRIAYIN MTGYPVTSVF
LLFYLLFPVI WIFRGIFYIQ KPFPTYVLYL VIVIFMSEMI GMVEIKWAGL TLLDWIRNEQ
FYIIGATAVY PLAVLHIVLK CFGLKGVSFK LTAKQVASST SEKFAELYDV QWAPLLFPTI
VVIAVNICAI GAAIGKALFG GWSLMQMGDA SLGLVFNVWI LLLIYPFALG IMGRWSKRPY
ILFVLIVISF VIIALADIAI QAMRSGSVRL HFRRSGGANF PTSWGF
