CSM1_YEAST
ID CSM1_YEAST Reviewed; 190 AA.
AC P25651; D6VR86;
DT 01-MAY-1992, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1992, sequence version 1.
DT 03-AUG-2022, entry version 171.
DE RecName: Full=Monopolin complex subunit CSM1;
DE AltName: Full=Chromosome segregation in meiosis protein 1;
GN Name=CSM1; Synonyms=SPO86; OrderedLocusNames=YCR086W; ORFNames=YCR86W;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=1574125; DOI=10.1038/357038a0;
RA Oliver S.G., van der Aart Q.J.M., Agostoni-Carbone M.L., Aigle M.,
RA Alberghina L., Alexandraki D., Antoine G., Anwar R., Ballesta J.P.G.,
RA Benit P., Berben G., Bergantino E., Biteau N., Bolle P.-A.,
RA Bolotin-Fukuhara M., Brown A., Brown A.J.P., Buhler J.-M., Carcano C.,
RA Carignani G., Cederberg H., Chanet R., Contreras R., Crouzet M.,
RA Daignan-Fornier B., Defoor E., Delgado M.D., Demolder J., Doira C.,
RA Dubois E., Dujon B., Duesterhoeft A., Erdmann D., Esteban M., Fabre F.,
RA Fairhead C., Faye G., Feldmann H., Fiers W., Francingues-Gaillard M.-C.,
RA Franco L., Frontali L., Fukuhara H., Fuller L.J., Galland P., Gent M.E.,
RA Gigot D., Gilliquet V., Glansdorff N., Goffeau A., Grenson M., Grisanti P.,
RA Grivell L.A., de Haan M., Haasemann M., Hatat D., Hoenicka J.,
RA Hegemann J.H., Herbert C.J., Hilger F., Hohmann S., Hollenberg C.P.,
RA Huse K., Iborra F., Indge K.J., Isono K., Jacq C., Jacquet M., James C.M.,
RA Jauniaux J.-C., Jia Y., Jimenez A., Kelly A., Kleinhans U., Kreisl P.,
RA Lanfranchi G., Lewis C., van der Linden C.G., Lucchini G.,
RA Lutzenkirchen K., Maat M.J., Mallet L., Mannhaupt G., Martegani E.,
RA Mathieu A., Maurer C.T.C., McConnell D., McKee R.A., Messenguy F.,
RA Mewes H.-W., Molemans F., Montague M.A., Muzi Falconi M., Navas L.,
RA Newlon C.S., Noone D., Pallier C., Panzeri L., Pearson B.M., Perea J.,
RA Philippsen P., Pierard A., Planta R.J., Plevani P., Poetsch B., Pohl F.M.,
RA Purnelle B., Ramezani Rad M., Rasmussen S.W., Raynal A., Remacha M.A.,
RA Richterich P., Roberts A.B., Rodriguez F., Sanz E.,
RA Schaaff-Gerstenschlaeger I., Scherens B., Schweitzer B., Shu Y., Skala J.,
RA Slonimski P.P., Sor F., Soustelle C., Spiegelberg R., Stateva L.I.,
RA Steensma H.Y., Steiner S., Thierry A., Thireos G., Tzermia M.,
RA Urrestarazu L.A., Valle G., Vetter I., van Vliet-Reedijk J.C., Voet M.,
RA Volckaert G., Vreken P., Wang H., Warmington J.R., von Wettstein D.,
RA Wicksteed B.L., Wilson C., Wurst H., Xu G., Yoshikawa A., Zimmermann F.K.,
RA Sgouros J.G.;
RT "The complete DNA sequence of yeast chromosome III.";
RL Nature 357:38-46(1992).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [4]
RP FUNCTION.
RX PubMed=11470404; DOI=10.1016/s0960-9822(01)00274-3;
RA Rabitsch K.P., Toth A., Galova M., Schleiffer A., Schaffner G., Aigner E.,
RA Rupp C., Penkner A.M., Moreno-Borchart A.C., Primig M., Esposito R.E.,
RA Klein F., Knop M., Nasmyth K.;
RT "A screen for genes required for meiosis and spore formation based on
RT whole-genome expression.";
RL Curr. Biol. 11:1001-1009(2001).
RN [5]
RP INTERACTION WITH GIC1 AND GIC2, AND SUBCELLULAR LOCATION.
RX PubMed=11489916; DOI=10.1083/jcb.200104057;
RA Drees B.L., Sundin B.A., Brazeau E., Caviston J.P., Chen G.-C., Guo W.,
RA Kozminski K.G., Lau M.W., Moskow J.J., Tong A., Schenkman L.R.,
RA McKenzie A. III, Brennwald P.J., Longtine M., Bi E., Chan C., Novick P.,
RA Boone C., Pringle J.R., Davis T.N., Fields S., Drubin D.G.;
RT "A protein interaction map for cell polarity development.";
RL J. Cell Biol. 154:549-571(2001).
RN [6]
RP FUNCTION, SUBCELLULAR LOCATION, AND IDENTIFICATION IN THE MONOPOLIN
RP COMPLEX.
RX PubMed=12689592; DOI=10.1016/s1534-5807(03)00086-8;
RA Rabitsch K.P., Petronczki M., Javerzat J.-P., Genier S., Chwalla B.,
RA Schleiffer A., Tanaka T.U., Nasmyth K.;
RT "Kinetochore recruitment of two nucleolar proteins is required for homolog
RT segregation in meiosis I.";
RL Dev. Cell 4:535-548(2003).
RN [7]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=14562095; DOI=10.1038/nature02026;
RA Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA Weissman J.S., O'Shea E.K.;
RT "Global analysis of protein localization in budding yeast.";
RL Nature 425:686-691(2003).
RN [8]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [9]
RP FUNCTION, SUBCELLULAR LOCATION, AND INTERACTION WITH CDC46; CDC47 AND MCM3.
RX PubMed=15023545; DOI=10.1016/j.yexcr.2003.12.008;
RA Wysocka M., Rytka J., Kurlandzka A.;
RT "Saccharomyces cerevisiae CSM1 gene encoding a protein influencing
RT chromosome segregation in meiosis I interacts with elements of the DNA
RT replication complex.";
RL Exp. Cell Res. 294:592-602(2004).
RN [10]
RP FUNCTION.
RX PubMed=16487579; DOI=10.1016/j.cell.2005.12.036;
RA Pan X., Ye P., Yuan D.S., Wang X., Bader J.S., Boeke J.D.;
RT "A DNA integrity network in the yeast Saccharomyces cerevisiae.";
RL Cell 124:1069-1081(2006).
RN [11]
RP FUNCTION, AND INTERACTION WITH NUR1.
RX PubMed=18997772; DOI=10.1038/nature07460;
RA Mekhail K., Seebacher J., Gygi S.P., Moazed D.;
RT "Role for perinuclear chromosome tethering in maintenance of genome
RT stability.";
RL Nature 456:667-670(2008).
CC -!- FUNCTION: Component of the monopolin complex which promotes
CC monoorientation during meiosis I, required for chromosome segregation
CC during meiosis. Also plays a mitotic role in DNA replication.
CC {ECO:0000269|PubMed:11470404, ECO:0000269|PubMed:12689592,
CC ECO:0000269|PubMed:15023545, ECO:0000269|PubMed:16487579,
CC ECO:0000269|PubMed:18997772}.
CC -!- SUBUNIT: Component of the monopolin complex composed of at least CSM1,
CC LRS4 and MAM1. The complex associates with the kinetochore. Interacts
CC with CDC46, CDC47, GIC1, GIC2, MCM3 and NUR1.
CC {ECO:0000269|PubMed:11489916, ECO:0000269|PubMed:12689592,
CC ECO:0000269|PubMed:15023545, ECO:0000269|PubMed:18997772}.
CC -!- INTERACTION:
CC P25651; P25651: CSM1; NbExp=3; IntAct=EBI-22001, EBI-22001;
CC P25651; P40568: DSN1; NbExp=3; IntAct=EBI-22001, EBI-25398;
CC P25651; Q04087: LRS4; NbExp=16; IntAct=EBI-22001, EBI-32189;
CC P25651; P40065: MAM1; NbExp=7; IntAct=EBI-22001, EBI-22643;
CC P25651; P24279: MCM3; NbExp=2; IntAct=EBI-22001, EBI-10541;
CC P25651; P29496: MCM5; NbExp=2; IntAct=EBI-22001, EBI-10549;
CC P25651; P38132: MCM7; NbExp=3; IntAct=EBI-22001, EBI-4300;
CC P25651; Q02208: TOF2; NbExp=4; IntAct=EBI-22001, EBI-27048;
CC -!- SUBCELLULAR LOCATION: Nucleus, nucleolus. Chromosome, centromere.
CC Note=Transiently released from the nucleolus and localizes to the
CC centromere regions during late pachytene.
CC -!- MISCELLANEOUS: Present with 1920 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
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DR EMBL; X59720; CAA42261.1; -; Genomic_DNA.
DR EMBL; AY558153; AAS56479.1; -; Genomic_DNA.
DR EMBL; BK006937; DAA07555.1; -; Genomic_DNA.
DR PIR; S19501; S19501.
DR RefSeq; NP_010009.1; NM_001178792.1.
DR PDB; 3N4R; X-ray; 2.60 A; A/B/C/D=69-181.
DR PDB; 3N4S; X-ray; 2.35 A; A/B/C/D=69-181.
DR PDB; 3N4X; X-ray; 3.41 A; A/B/C/D=1-190.
DR PDB; 3N7N; X-ray; 3.90 A; A/B/C/D=1-190.
DR PDB; 5KTB; X-ray; 3.05 A; A/B=1-190.
DR PDB; 5V1A; X-ray; 2.14 A; A=69-190.
DR PDB; 5V3N; X-ray; 1.30 A; A=69-181.
DR PDB; 6DEI; X-ray; 1.70 A; A/B=69-181.
DR PDBsum; 3N4R; -.
DR PDBsum; 3N4S; -.
DR PDBsum; 3N4X; -.
DR PDBsum; 3N7N; -.
DR PDBsum; 5KTB; -.
DR PDBsum; 5V1A; -.
DR PDBsum; 5V3N; -.
DR PDBsum; 6DEI; -.
DR AlphaFoldDB; P25651; -.
DR SMR; P25651; -.
DR BioGRID; 31058; 468.
DR ComplexPortal; CPX-1681; Monopolin complex.
DR DIP; DIP-1283N; -.
DR IntAct; P25651; 55.
DR MINT; P25651; -.
DR STRING; 4932.YCR086W; -.
DR MaxQB; P25651; -.
DR PaxDb; P25651; -.
DR PRIDE; P25651; -.
DR EnsemblFungi; YCR086W_mRNA; YCR086W; YCR086W.
DR GeneID; 850447; -.
DR KEGG; sce:YCR086W; -.
DR SGD; S000000682; CSM1.
DR VEuPathDB; FungiDB:YCR086W; -.
DR eggNOG; ENOG502RZY3; Eukaryota.
DR HOGENOM; CLU_100702_0_0_1; -.
DR InParanoid; P25651; -.
DR OMA; GLWFDTS; -.
DR BioCyc; YEAST:G3O-29381-MON; -.
DR EvolutionaryTrace; P25651; -.
DR PRO; PR:P25651; -.
DR Proteomes; UP000002311; Chromosome III.
DR RNAct; P25651; protein.
DR GO; GO:0034506; C:chromosome, centromeric core domain; IBA:GO_Central.
DR GO; GO:0072686; C:mitotic spindle; IBA:GO_Central.
DR GO; GO:0033551; C:monopolin complex; IDA:SGD.
DR GO; GO:0005635; C:nuclear envelope; IDA:SGD.
DR GO; GO:0005730; C:nucleolus; IDA:SGD.
DR GO; GO:0042802; F:identical protein binding; IPI:IntAct.
DR GO; GO:1990644; F:microtubule site clamp; IBA:GO_Central.
DR GO; GO:0051315; P:attachment of mitotic spindle microtubules to kinetochore; IBA:GO_Central.
DR GO; GO:0045143; P:homologous chromosome segregation; IMP:SGD.
DR GO; GO:0045132; P:meiotic chromosome segregation; IMP:SGD.
DR GO; GO:0045144; P:meiotic sister chromatid segregation; IBA:GO_Central.
DR GO; GO:0051455; P:monopolar spindle attachment to meiosis I kinetochore; IGI:SGD.
DR GO; GO:0034503; P:protein localization to nucleolar rDNA repeats; IMP:SGD.
DR GO; GO:0070550; P:rDNA chromatin condensation; IMP:SGD.
DR Gene3D; 3.90.1150.80; -; 1.
DR InterPro; IPR040349; Csm1/Pcs1.
DR InterPro; IPR020981; Csm1/Pcs1_C.
DR InterPro; IPR038608; Csm1/Pcs1_C_sf.
DR InterPro; IPR041671; Csm1_N.
DR PANTHER; PTHR28006; PTHR28006; 1.
DR Pfam; PF12539; Csm1; 1.
DR Pfam; PF18504; Csm1_N; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Centromere; Chromosome; Coiled coil; Meiosis; Nucleus;
KW Reference proteome.
FT CHAIN 1..190
FT /note="Monopolin complex subunit CSM1"
FT /id="PRO_0000202577"
FT COILED 31..70
FT /evidence="ECO:0000255"
FT HELIX 11..15
FT /evidence="ECO:0007829|PDB:3N4X"
FT HELIX 71..82
FT /evidence="ECO:0007829|PDB:5V3N"
FT STRAND 83..91
FT /evidence="ECO:0007829|PDB:5V3N"
FT STRAND 96..104
FT /evidence="ECO:0007829|PDB:5V3N"
FT STRAND 114..124
FT /evidence="ECO:0007829|PDB:5V3N"
FT HELIX 126..128
FT /evidence="ECO:0007829|PDB:5V3N"
FT STRAND 130..136
FT /evidence="ECO:0007829|PDB:5V3N"
FT HELIX 138..140
FT /evidence="ECO:0007829|PDB:6DEI"
FT HELIX 143..152
FT /evidence="ECO:0007829|PDB:5V3N"
FT HELIX 155..158
FT /evidence="ECO:0007829|PDB:5V3N"
FT STRAND 161..164
FT /evidence="ECO:0007829|PDB:5V3N"
FT HELIX 165..167
FT /evidence="ECO:0007829|PDB:5V3N"
FT HELIX 168..179
FT /evidence="ECO:0007829|PDB:5V3N"
SQ SEQUENCE 190 AA; 21751 MW; 9650E0DA89B31CC5 CRC64;
MDPLTVYKNS VKQQIDSADL LVANLVNENF VLSEKLDTKA TEIKQLQKQI DSLNAQVKEL
KTQTSQQAEN SEVIKDLYEY LCNVRVHKSY EDDSGLWFDI SQGTHSGGSS DDYSIMDYKL
GFVKGQAQVT EVIYAPVLKQ RSTEELYSLQ SKLPEYLFET LSFPLSSLNQ FYNKIAKSLN
KKREKKDETE
