ID   CSM3_ENTI1              Reviewed;         214 AA.
AC   E6LHV5;
DT   16-JAN-2019, integrated into UniProtKB/Swiss-Prot.
DT   08-MAR-2011, sequence version 1.
DT   25-MAY-2022, entry version 30.
DE   RecName: Full=CRISPR system Cms endoribonuclease Csm3;
DE            EC=3.1.-.- {ECO:0000269|PubMed:28722012};
DE   AltName: Full=CRISPR type III A-associated RAMP protein Csm3;
GN   Name=csm3; ORFNames=HMPREF9088_1945;
OS   Enterococcus italicus (strain DSM 15952 / CCUG 50447 / LMG 22039 / TP 1.5).
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
OC   Enterococcus.
OX   NCBI_TaxID=888064;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 15952 / CCUG 50447 / LMG 22039 / TP 1.5;
RA   Muzny D., Qin X., Deng J., Jiang H., Liu Y., Qu J., Song X.-Z., Zhang L.,
RA   Thornton R., Coyle M., Francisco L., Jackson L., Javaid M., Korchina V.,
RA   Kovar C., Mata R., Mathew T., Ngo R., Nguyen L., Nguyen N., Okwuonu G.,
RA   Ongeri F., Pham C., Simmons D., Wilczek-Boney K., Hale W., Jakkamsetti A.,
RA   Pham P., Ruth R., San Lucas F., Warren J., Zhang J., Zhao Z., Zhou C.,
RA   Zhu D., Lee S., Bess C., Blankenburg K., Forbes L., Fu Q., Gubbala S.,
RA   Hirani K., Jayaseelan J.C., Lara F., Munidasa M., Palculict T., Patil S.,
RA   Pu L.-L., Saada N., Tang L., Weissenberger G., Zhu Y., Hemphill L.,
RA   Shang Y., Youmans B., Ayvaz T., Ross M., Santibanez J., Aqrawi P.,
RA   Gross S., Joshi V., Fowler G., Nazareth L., Reid J., Worley K.,
RA   Petrosino J., Highlander S., Gibbs R.;
RL   Submitted (DEC-2010) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   FUNCTION IN PHAGE RESISTANCE, SUBUNIT, AND MUTAGENESIS OF ASP-32.
RC   STRAIN=DSM 15952 / CCUG 50447 / LMG 22039 / TP 1.5;
RX   PubMed=28722012; DOI=10.1038/nature23467;
RA   Niewoehner O., Garcia-Doval C., Rostoel J.T., Berk C., Schwede F.,
RA   Bigler L., Hall J., Marraffini L.A., Jinek M.;
RT   "Type III CRISPR-Cas systems produce cyclic oligoadenylate second
RT   messengers.";
RL   Nature 548:543-548(2017).
CC   -!- FUNCTION: CRISPR (clustered regularly interspaced short palindromic
CC       repeat) is an adaptive immune system that provides protection against
CC       mobile genetic elements (viruses, transposable elements and conjugative
CC       plasmids). CRISPR clusters contain spacers, sequences complementary to
CC       antecedent mobile elements, and target invading nucleic acids. CRISPR
CC       clusters are transcribed and processed into CRISPR RNA (crRNA). The
CC       type III-A Csm effector complex binds crRNA and acts as a crRNA-guided
CC       RNase, DNase and cyclic oligoadenylate synthase; binding of target RNA
CC       cognate to the crRNA is required for all activities. In a heterologous
CC       host the appropriately targeted Csm effector complex prevents growth of
CC       dsDNA phage phiNM1-gamma6. {ECO:0000269|PubMed:28722012}.
CC   -!- FUNCTION: This subunit has the target ssRNA endonuclease activity; it
CC       cleaves multiple sites in the target RNA at 6 nucleotide intervals.
CC       {ECO:0000269|PubMed:28722012}.
CC   -!- SUBUNIT: Part of the Csm effector complex that includes Cas10, Csm2,
CC       Csm3, Csm4 and Csm5. {ECO:0000269|PubMed:28722012}.
CC   -!- MISCELLANEOUS: Encoded in a type III-A CRISPR locus.
CC       {ECO:0000269|PubMed:28722012}.
CC   -!- SIMILARITY: Belongs to the CRISPR-associated Csm3 family.
CC       {ECO:0000305}.
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DR   EMBL; AEPV01000074; EFU73215.1; -; Genomic_DNA.
DR   RefSeq; WP_007208956.1; NZ_JXKT01000007.1.
DR   AlphaFoldDB; E6LHV5; -.
DR   SMR; E6LHV5; -.
DR   STRING; 888064.HMPREF9088_1945; -.
DR   EnsemblBacteria; EFU73215; EFU73215; HMPREF9088_1945.
DR   PATRIC; fig|888064.11.peg.2083; -.
DR   eggNOG; COG1337; Bacteria.
DR   HOGENOM; CLU_067743_0_0_9; -.
DR   OMA; NPVIKDP; -.
DR   OrthoDB; 1840881at2; -.
DR   Proteomes; UP000010296; Unassembled WGS sequence.
DR   GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-KW.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0051607; P:defense response to virus; IEA:UniProtKB-KW.
DR   InterPro; IPR013412; CRISPR-assoc_RAMP_Csm3.
DR   InterPro; IPR005537; RAMP_III_fam.
DR   Pfam; PF03787; RAMPs; 1.
DR   TIGRFAMs; TIGR02582; cas7_TM1809; 1.
PE   1: Evidence at protein level;
KW   Antiviral defense; Endonuclease; Hydrolase; Nuclease; Reference proteome;
KW   RNA-binding.
FT   CHAIN           1..214
FT                   /note="CRISPR system Cms endoribonuclease Csm3"
FT                   /id="PRO_0000446118"
FT   MUTAGEN         32
FT                   /note="D->A: No degradation of target ssRNA by the Csm
FT                   complex, complex assembles normally and synthesizes cA6
FT                   normally."
FT                   /evidence="ECO:0000269|PubMed:28722012"
SQ   SEQUENCE   214 AA;  23514 MW;  C1610BF2C7C18104 CRC64;
     MYSKIRIVGK IDVLTGLHIG GGGETSMIGA IDSPVVRDPY SRLPIIPGSS IKGKMRSLLA
     KHIGLIPGQK MHNQDAPEIL RLFGSSQKGA IQSSRLQISD AFFSKASQEE FDKKDLAYTE
     TKFENTISRL TAVANPRQIE RVTRGASFDF HIIYNVENIN EVMADFENIK TAIHLLENDY
     LGGGGTRGNG RIRFVIDSID TVVGDFDSSN LSIK