ID   CSM3_METTH              Reviewed;         245 AA.
AC   O27152;
DT   02-FEB-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1998, sequence version 1.
DT   25-MAY-2022, entry version 103.
DE   RecName: Full=CRISPR system Cms endoribonuclease Csm3;
DE            EC=3.1.-.-;
DE   AltName: Full=CRISPR type III A-associated RAMP protein Csm3;
GN   Name=csm3; OrderedLocusNames=MTH_1080;
OS   Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM
OS   10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum).
OC   Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
OC   Methanobacteriales; Methanobacteriaceae; Methanothermobacter.
OX   NCBI_TaxID=187420;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H;
RX   PubMed=9371463; DOI=10.1128/jb.179.22.7135-7155.1997;
RA   Smith D.R., Doucette-Stamm L.A., Deloughery C., Lee H.-M., Dubois J.,
RA   Aldredge T., Bashirzadeh R., Blakely D., Cook R., Gilbert K., Harrison D.,
RA   Hoang L., Keagle P., Lumm W., Pothier B., Qiu D., Spadafora R., Vicare R.,
RA   Wang Y., Wierzbowski J., Gibson R., Jiwani N., Caruso A., Bush D.,
RA   Safer H., Patwell D., Prabhakar S., McDougall S., Shimer G., Goyal A.,
RA   Pietrovski S., Church G.M., Daniels C.J., Mao J.-I., Rice P., Noelling J.,
RA   Reeve J.N.;
RT   "Complete genome sequence of Methanobacterium thermoautotrophicum deltaH:
RT   functional analysis and comparative genomics.";
RL   J. Bacteriol. 179:7135-7155(1997).
CC   -!- FUNCTION: CRISPR (clustered regularly interspaced short palindromic
CC       repeat) is an adaptive immune system that provides protection against
CC       mobile genetic elements (viruses, transposable elements and conjugative
CC       plasmids). CRISPR clusters contain spacers, sequences complementary to
CC       antecedent mobile elements, and target invading nucleic acids. CRISPR
CC       clusters are transcribed and processed into CRISPR RNA (crRNA). The
CC       type III-A Csm effector complex binds crRNA and acts as a crRNA-guided
CC       RNase, DNase and cyclic oligoadenylate synthase; binding of target RNA
CC       cognate to the crRNA is required for all activities.
CC       {ECO:0000250|UniProtKB:A0A0A7HIF0}.
CC   -!- FUNCTION: This subunit has the target ssRNA endonuclease activity; it
CC       cleaves multiple sites in the target RNA at 6 nucleotide intervals.
CC       {ECO:0000250|UniProtKB:A0A0A7HIF0}.
CC   -!- SUBUNIT: Part of the Csm effector complex that includes Cas10, Csm2,
CC       Csm3, Csm4 and Csm5. {ECO:0000250|UniProtKB:A0A0A7HIF0}.
CC   -!- MISCELLANEOUS: Encoded in a type III-A CRISPR locus. {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the CRISPR-associated Csm3 family.
CC       {ECO:0000305}.
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DR   EMBL; AE000666; AAB85569.1; -; Genomic_DNA.
DR   PIR; E69010; E69010.
DR   RefSeq; WP_010876704.1; NC_000916.1.
DR   AlphaFoldDB; O27152; -.
DR   SMR; O27152; -.
DR   EnsemblBacteria; AAB85569; AAB85569; MTH_1080.
DR   GeneID; 1471488; -.
DR   KEGG; mth:MTH_1080; -.
DR   PATRIC; fig|187420.15.peg.1058; -.
DR   HOGENOM; CLU_067743_0_0_2; -.
DR   OMA; NPVIKDP; -.
DR   Proteomes; UP000005223; Chromosome.
DR   GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-KW.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0051607; P:defense response to virus; IEA:UniProtKB-KW.
DR   CDD; cd09684; Csm3_III-A; 1.
DR   InterPro; IPR013412; CRISPR-assoc_RAMP_Csm3.
DR   InterPro; IPR005537; RAMP_III_fam.
DR   Pfam; PF03787; RAMPs; 1.
DR   TIGRFAMs; TIGR02582; cas7_TM1809; 1.
PE   3: Inferred from homology;
KW   Antiviral defense; Endonuclease; Hydrolase; Nuclease; Reference proteome;
KW   RNA-binding.
FT   CHAIN           1..245
FT                   /note="CRISPR system Cms endoribonuclease Csm3"
FT                   /id="PRO_0000107467"
FT   REGION          88..110
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   245 AA;  26979 MW;  A2B73C21B42A0F35 CRC64;
     MRFQKNYIIT GEILCRTGLH IGVSKDSIEI GGSDNPIIRD PVTRLPYIPG SSIKGKMRSL
     LELELDRVSN GGPCKCGKCE ICRVFGSAAD SSSSSGPTRT DSSSSSGPTR IIVRDAFPTD
     ETVEEWKESS EVVEGAELKY ENNLNRITSM ANPRNQERVP RGSKFGFEII VSEYDGDSDN
     LRIVLEGLRL LEDSYLGGSG TRGYGKIEFK NIKIRERPVE YYRGEAGEST MGDFESLADI
     NTTAE