ID   CSM3_MYCTO              Reviewed;         236 AA.
AC   P9WJF8; F2GLB3; L0TAZ2; P71631; Q7D6I3;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2014, sequence version 1.
DT   25-MAY-2022, entry version 33.
DE   RecName: Full=CRISPR system Cms endoribonuclease Csm3;
DE            EC=3.1.-.-;
DE   AltName: Full=CRISPR type III A-associated RAMP protein Csm3;
GN   Name=csm3; OrderedLocusNames=MT2888;
OS   Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83331;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CDC 1551 / Oshkosh;
RX   PubMed=12218036; DOI=10.1128/jb.184.19.5479-5490.2002;
RA   Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O.,
RA   Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K.,
RA   Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L.,
RA   Delcher A., Utterback T.R., Weidman J.F., Khouri H.M., Gill J., Mikula A.,
RA   Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M.;
RT   "Whole-genome comparison of Mycobacterium tuberculosis clinical and
RT   laboratory strains.";
RL   J. Bacteriol. 184:5479-5490(2002).
CC   -!- FUNCTION: CRISPR (clustered regularly interspaced short palindromic
CC       repeat) is an adaptive immune system that provides protection against
CC       mobile genetic elements (viruses, transposable elements and conjugative
CC       plasmids). CRISPR clusters contain spacers, sequences complementary to
CC       antecedent mobile elements, and target invading nucleic acids. CRISPR
CC       clusters are transcribed and processed into CRISPR RNA (crRNA). The
CC       type III-A Csm effector complex binds crRNA and acts as a crRNA-guided
CC       RNase, DNase and cyclic oligoadenylate synthase; binding of target RNA
CC       cognate to the crRNA is required for all activities.
CC       {ECO:0000250|UniProtKB:A0A0A7HIF0}.
CC   -!- FUNCTION: This subunit has the target ssRNA endonuclease activity; it
CC       cleaves multiple sites in the target RNA at 6 nucleotide intervals.
CC       {ECO:0000250|UniProtKB:A0A0A7HIF0}.
CC   -!- SUBUNIT: Part of the Csm effector complex that includes Cas10, Csm2,
CC       Csm3, Csm4 and Csm5. {ECO:0000250|UniProtKB:P9WJF9}.
CC   -!- MISCELLANEOUS: Encoded in a type III-A CRISPR locus. {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the CRISPR-associated Csm3 family.
CC       {ECO:0000305}.
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DR   EMBL; AE000516; AAK47213.1; -; Genomic_DNA.
DR   PIR; H70691; H70691.
DR   RefSeq; WP_003414292.1; NZ_KK341227.1.
DR   AlphaFoldDB; P9WJF8; -.
DR   SMR; P9WJF8; -.
DR   EnsemblBacteria; AAK47213; AAK47213; MT2888.
DR   KEGG; mtc:MT2888; -.
DR   PATRIC; fig|83331.31.peg.3117; -.
DR   HOGENOM; CLU_067743_0_0_11; -.
DR   Proteomes; UP000001020; Chromosome.
DR   GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-KW.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0051607; P:defense response to virus; IEA:UniProtKB-KW.
DR   CDD; cd09684; Csm3_III-A; 1.
DR   InterPro; IPR013412; CRISPR-assoc_RAMP_Csm3.
DR   InterPro; IPR005537; RAMP_III_fam.
DR   Pfam; PF03787; RAMPs; 1.
DR   TIGRFAMs; TIGR02582; cas7_TM1809; 1.
PE   3: Inferred from homology;
KW   Antiviral defense; Endonuclease; Hydrolase; Nuclease; RNA-binding.
FT   CHAIN           1..236
FT                   /note="CRISPR system Cms endoribonuclease Csm3"
FT                   /id="PRO_0000427837"
SQ   SEQUENCE   236 AA;  25773 MW;  1728D20400321017 CRC64;
     MTTSYAKIEI TGTLTVLTGL QIGAGDGFSA IGAVDKPVVR DPLSRLPMIP GTSLKGKVRT
     LLSRQYGADT ETFYRKPNED HAHIRRLFGD TEEYMTGRLV FRDTKLTNKD DLEARGAKTL
     TEVKFENAIN RVTAKANLRQ MERVIPGSEF AFSLVYEVSF GTPGEEQKAS LPSSDEIIED
     FNAIARGLKL LELDYLGGSG TRGYGQVKFS NLKARAAVGA LDGSLLEKLN HELAAV