CSM4_ENTI1
ID CSM4_ENTI1 Reviewed; 307 AA.
AC E6LHV4;
DT 16-JAN-2019, integrated into UniProtKB/Swiss-Prot.
DT 08-MAR-2011, sequence version 1.
DT 25-MAY-2022, entry version 35.
DE RecName: Full=CRISPR system Cms protein Csm4;
DE AltName: Full=CRISPR type III A-associated RAMP protein Csm4;
GN Name=csm4; ORFNames=HMPREF9088_1944;
OS Enterococcus italicus (strain DSM 15952 / CCUG 50447 / LMG 22039 / TP 1.5).
OC Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
OC Enterococcus.
OX NCBI_TaxID=888064;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 15952 / CCUG 50447 / LMG 22039 / TP 1.5;
RA Muzny D., Qin X., Deng J., Jiang H., Liu Y., Qu J., Song X.-Z., Zhang L.,
RA Thornton R., Coyle M., Francisco L., Jackson L., Javaid M., Korchina V.,
RA Kovar C., Mata R., Mathew T., Ngo R., Nguyen L., Nguyen N., Okwuonu G.,
RA Ongeri F., Pham C., Simmons D., Wilczek-Boney K., Hale W., Jakkamsetti A.,
RA Pham P., Ruth R., San Lucas F., Warren J., Zhang J., Zhao Z., Zhou C.,
RA Zhu D., Lee S., Bess C., Blankenburg K., Forbes L., Fu Q., Gubbala S.,
RA Hirani K., Jayaseelan J.C., Lara F., Munidasa M., Palculict T., Patil S.,
RA Pu L.-L., Saada N., Tang L., Weissenberger G., Zhu Y., Hemphill L.,
RA Shang Y., Youmans B., Ayvaz T., Ross M., Santibanez J., Aqrawi P.,
RA Gross S., Joshi V., Fowler G., Nazareth L., Reid J., Worley K.,
RA Petrosino J., Highlander S., Gibbs R.;
RL Submitted (DEC-2010) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP FUNCTION IN PHAGE RESISTANCE, AND SUBUNIT.
RC STRAIN=DSM 15952 / CCUG 50447 / LMG 22039 / TP 1.5;
RX PubMed=28722012; DOI=10.1038/nature23467;
RA Niewoehner O., Garcia-Doval C., Rostoel J.T., Berk C., Schwede F.,
RA Bigler L., Hall J., Marraffini L.A., Jinek M.;
RT "Type III CRISPR-Cas systems produce cyclic oligoadenylate second
RT messengers.";
RL Nature 548:543-548(2017).
CC -!- FUNCTION: CRISPR (clustered regularly interspaced short palindromic
CC repeat) is an adaptive immune system that provides protection against
CC mobile genetic elements (viruses, transposable elements and conjugative
CC plasmids). CRISPR clusters contain spacers, sequences complementary to
CC antecedent mobile elements, and target invading nucleic acids. CRISPR
CC clusters are transcribed and processed into CRISPR RNA (crRNA). The
CC type III-A Csm effector complex binds crRNA and acts as a crRNA-guided
CC RNase, DNase and cyclic oligoadenylate synthase; binding of target RNA
CC cognate to the crRNA is required for all activities. In a heterologous
CC host the appropriately targeted Csm effector complex prevents growth of
CC dsDNA phage phiNM1-gamma6. {ECO:0000269|PubMed:28722012}.
CC -!- FUNCTION: The subunit probably binds to the 5' handle of the crRNA,
CC helping in discrimination between self- and non-self.
CC {ECO:0000250|UniProtKB:A0A0A7HGA1}.
CC -!- SUBUNIT: Part of the Csm effector complex that includes Cas10, Csm2,
CC Csm3, Csm4 and Csm5. {ECO:0000269|PubMed:28722012}.
CC -!- MISCELLANEOUS: Encoded in a type III-A CRISPR locus.
CC {ECO:0000269|PubMed:28722012}.
CC -!- SIMILARITY: Belongs to the CRISPR-associated Csm4 family.
CC {ECO:0000305}.
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DR EMBL; AEPV01000074; EFU73214.1; -; Genomic_DNA.
DR AlphaFoldDB; E6LHV4; -.
DR SMR; E6LHV4; -.
DR STRING; 888064.HMPREF9088_1944; -.
DR EnsemblBacteria; EFU73214; EFU73214; HMPREF9088_1944.
DR PATRIC; fig|888064.11.peg.2082; -.
DR eggNOG; COG1567; Bacteria.
DR HOGENOM; CLU_062371_1_0_9; -.
DR OMA; YFSHFEF; -.
DR Proteomes; UP000010296; Unassembled WGS sequence.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0051607; P:defense response to virus; IEA:UniProtKB-KW.
DR InterPro; IPR005510; Csm4.
DR InterPro; IPR040932; Csm4_C.
DR Pfam; PF17953; Csm4_C; 1.
DR TIGRFAMs; TIGR01903; cas5_csm4; 1.
PE 1: Evidence at protein level;
KW Antiviral defense; Reference proteome; RNA-binding.
FT CHAIN 1..307
FT /note="CRISPR system Cms protein Csm4"
FT /id="PRO_0000446120"
SQ SEQUENCE 307 AA; 34316 MW; 5C0D52EC323B7AEC CRC64;
MNQLVVKLVK LTFKSPVHFG MKRLSDSNHT IAADTLFSAL IIEALQQQLE LSHLLNNLVI
TDLFPYNKTS YFLPKPLIRI EGKKGDESGY KAFKKLTYIP VENYSEYLRG EIDSLEASKI
AESLNLGKAS LSTKVSLQAV DHNGESEPYS VGNFTFYPES GLYFLAKGNA DTIGQLEILM
HALQYSGIGG KRSAGYGQFR CTIEDSGKFD SLLSQTGNIA ILLSSAMASD EELVDCLEDA
RYLLKKRTGF VQSKTYADQL VKKKDFYAFS AGSTFYQKFN GKIFDVSDNG RHSVYRYAKA
FWLEGKI