CSM4_MYCTU
ID CSM4_MYCTU Reviewed; 302 AA.
AC P9WJF7; F2GLB4; L0TAR3; P71632; Q7D6I4;
DT 16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT 16-APR-2014, sequence version 1.
DT 25-MAY-2022, entry version 32.
DE RecName: Full=CRISPR system Cms protein Csm4;
DE AltName: Full=CRISPR type III-A associated RAMP protein Csm4;
GN Name=csm4; OrderedLocusNames=Rv2820c;
OS Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC Mycobacterium; Mycobacterium tuberculosis complex.
OX NCBI_TaxID=83332;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 25618 / H37Rv;
RX PubMed=9634230; DOI=10.1038/31159;
RA Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA Barrell B.G.;
RT "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT genome sequence.";
RL Nature 393:537-544(1998).
RN [2]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC STRAIN=ATCC 25618 / H37Rv;
RX PubMed=21969609; DOI=10.1074/mcp.m111.011627;
RA Kelkar D.S., Kumar D., Kumar P., Balakrishnan L., Muthusamy B., Yadav A.K.,
RA Shrivastava P., Marimuthu A., Anand S., Sundaram H., Kingsbury R.,
RA Harsha H.C., Nair B., Prasad T.S., Chauhan D.S., Katoch K., Katoch V.M.,
RA Kumar P., Chaerkady R., Ramachandran S., Dash D., Pandey A.;
RT "Proteogenomic analysis of Mycobacterium tuberculosis by high resolution
RT mass spectrometry.";
RL Mol. Cell. Proteomics 10:M111.011627-M111.011627(2011).
RN [3]
RP FUNCTION IN PLASMID RESISTANCE, SUBUNIT, AND DISRUPTION PHENOTYPE.
RC STRAIN=H37Rv;
RX PubMed=29979631; DOI=10.1096/fj.201800557rr;
RA Wei W., Zhang S., Fleming J., Chen Y., Li Z., Fan S., Liu Y., Wang W.,
RA Wang T., Liu Y., Ren B., Wang M., Jiao J., Chen Y., Zhou Y., Zhou Y.,
RA Gu S., Zhang X., Wan L., Chen T., Zhou L., Chen Y., Zhang X.E., Li C.,
RA Zhang H., Bi L.;
RT "Mycobacterium tuberculosis type III-A CRISPR/Cas system crRNA and its
RT maturation have atypical features.";
RL FASEB J. 33:1496-1509(2019).
CC -!- FUNCTION: CRISPR (clustered regularly interspaced short palindromic
CC repeat) is an adaptive immune system that provides protection against
CC mobile genetic elements (viruses, transposable elements and conjugative
CC plasmids). CRISPR clusters contain spacers, sequences complementary to
CC antecedent mobile elements, and target invading nucleic acids. CRISPR
CC clusters are transcribed and processed into CRISPR RNA (crRNA). The
CC type III-A Csm effector complex binds crRNA and acts as a crRNA-guided
CC RNase, DNase and cyclic oligoadenylate synthase; binding of target RNA
CC cognate to the crRNA is required for all activities (Probable). This
CC CRISPR-Cas system protects bacteria against transformation with
CC plasmids containing DNA homologous to its spacer regions
CC (PubMed:29979631). {ECO:0000269|PubMed:29979631,
CC ECO:0000305|PubMed:29979631}.
CC -!- FUNCTION: The subunit probably binds to the 5' handle of the crRNA,
CC helping in discrimination between self- and non-self.
CC {ECO:0000250|UniProtKB:A0A0A7HGA1}.
CC -!- SUBUNIT: Part of the Csm effector complex that includes Cas10, Csm2,
CC Csm3, Csm4 and Csm5. {ECO:0000269|PubMed:29979631}.
CC -!- DISRUPTION PHENOTYPE: Deletion of the entire CRISPR-Cas locus (cas6 to
CC cas2, Rv2824c to Rv2816c) decreases resistance to plasmids encoding
CC spacer elements about 6-fold. {ECO:0000269|PubMed:29979631}.
CC -!- MISCELLANEOUS: Encoded in a type III-A CRISPR locus.
CC {ECO:0000305|PubMed:29979631}.
CC -!- SIMILARITY: Belongs to the CRISPR-associated Csm4 family.
CC {ECO:0000305}.
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DR EMBL; AL123456; CCP45620.1; -; Genomic_DNA.
DR PIR; G70691; G70691.
DR RefSeq; NP_217336.1; NC_000962.3.
DR RefSeq; WP_003414288.1; NZ_NVQJ01000006.1.
DR AlphaFoldDB; P9WJF7; -.
DR SMR; P9WJF7; -.
DR STRING; 83332.Rv2820c; -.
DR PaxDb; P9WJF7; -.
DR DNASU; 888908; -.
DR GeneID; 888908; -.
DR KEGG; mtu:Rv2820c; -.
DR TubercuList; Rv2820c; -.
DR eggNOG; COG1567; Bacteria.
DR OMA; ACCHEIS; -.
DR Proteomes; UP000001584; Chromosome.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0051607; P:defense response to virus; IEA:UniProtKB-KW.
DR InterPro; IPR005510; Csm4.
DR InterPro; IPR040932; Csm4_C.
DR Pfam; PF17953; Csm4_C; 1.
DR TIGRFAMs; TIGR01903; cas5_csm4; 1.
PE 1: Evidence at protein level;
KW Antiviral defense; Reference proteome; RNA-binding.
FT CHAIN 1..302
FT /note="CRISPR system Cms protein Csm4"
FT /id="PRO_0000418227"
SQ SEQUENCE 302 AA; 32301 MW; 7760E26BA3107802 CRC64;
MNSRLFRFDF DRTHFGDHGL ESSTISCPAD TLYSALCVEA LRMGGQQLLG ELVACSTLRL
TDLLPYVGPD YLVPKPLHSV RSDGSSMQKK LAKKIGFLPA AQLGSFLDGT ADLKELAARQ
TKIGVHAVSA KAAIHNGKKD ADPYRVGYFR FELDAGLWLL ATGSESELGL LTRLLKGISA
LGGERTSGFG AFNLTESEAP AALTPTVDAA SLMTLTTSLP TDDELEAALA GATYRLVKRS
GFVASSTYAD MPLRKRDIYK FAAGSVFSRP FQGGILDVSL GGNHPVYSYA RPLFLALPES
AA