CSM4_THEON
ID CSM4_THEON Reviewed; 289 AA.
AC B6YWC1;
DT 16-JAN-2019, integrated into UniProtKB/Swiss-Prot.
DT 20-JAN-2009, sequence version 1.
DT 25-MAY-2022, entry version 69.
DE RecName: Full=CRISPR system Cms protein Csm4;
DE AltName: Full=CRISPR type III A-associated RAMP protein Csm4;
GN Name=csm4 {ECO:0000303|PubMed:25773141}; OrderedLocusNames=TON_0896;
OS Thermococcus onnurineus (strain NA1).
OC Archaea; Euryarchaeota; Thermococci; Thermococcales; Thermococcaceae;
OC Thermococcus.
OX NCBI_TaxID=523850;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NA1;
RX PubMed=18790866; DOI=10.1128/jb.00746-08;
RA Lee H.S., Kang S.G., Bae S.S., Lim J.K., Cho Y., Kim Y.J., Jeon J.H.,
RA Cha S.-S., Kwon K.K., Kim H.-T., Park C.-J., Lee H.-W., Kim S.I., Chun J.,
RA Colwell R.R., Kim S.-J., Lee J.-H.;
RT "The complete genome sequence of Thermococcus onnurineus NA1 reveals a
RT mixed heterotrophic and carboxydotrophic metabolism.";
RL J. Bacteriol. 190:7491-7499(2008).
RN [2]
RP INTERACTION WITH CAS10 (CSM1), AND SUBUNIT.
RC STRAIN=NA1;
RX PubMed=25773141; DOI=10.1016/j.str.2015.01.021;
RA Jung T.Y., An Y., Park K.H., Lee M.H., Oh B.H., Woo E.;
RT "Crystal structure of the Csm1 subunit of the Csm complex and its single-
RT stranded DNA-specific nuclease activity.";
RL Structure 23:782-790(2015).
CC -!- FUNCTION: CRISPR (clustered regularly interspaced short palindromic
CC repeat) is an adaptive immune system that provides protection against
CC mobile genetic elements (viruses, transposable elements and conjugative
CC plasmids). CRISPR clusters contain spacers, sequences complementary to
CC antecedent mobile elements, and target invading nucleic acids. CRISPR
CC clusters are transcribed and processed into CRISPR RNA (crRNA). The
CC type III-A Csm effector complex binds crRNA and acts as a crRNA-guided
CC RNase, DNase and cyclic oligoadenylate synthase; binding of target RNA
CC cognate to the crRNA is required for all activities.
CC {ECO:0000250|UniProtKB:A0A0A7HGA1}.
CC -!- FUNCTION: The subunit probably binds to the 5' handle of the crRNA,
CC helping in discrimination between self- and non-self.
CC {ECO:0000250|UniProtKB:A0A0A7HGA1}.
CC -!- SUBUNIT: Probably part of the Csm effector complex, that includes
CC Cas10, Csm2, Csm3, Csm4, Csm5 and mature crRNA (By similarity).
CC Interacts with Cas10 (csm1) (PubMed:25773141).
CC {ECO:0000250|UniProtKB:A0A0A7HGA1, ECO:0000269|PubMed:25773141}.
CC -!- INTERACTION:
CC B6YWC1; B6YWB8: csm1; NbExp=2; IntAct=EBI-16149979, EBI-16149952;
CC -!- MISCELLANEOUS: Encoded in a type III-A CRISPR locus.
CC {ECO:0000305|PubMed:25773141}.
CC -!- SIMILARITY: Belongs to the CRISPR-associated Csm4 family.
CC {ECO:0000305}.
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DR EMBL; CP000855; ACJ16384.1; -; Genomic_DNA.
DR RefSeq; WP_012571856.1; NC_011529.1.
DR PDB; 6IQW; EM; 3.35 A; E=1-289.
DR PDB; 6MUA; X-ray; 2.91 A; B=1-289.
DR PDB; 6MUR; EM; 3.10 A; E=1-289.
DR PDB; 6MUS; EM; 3.60 A; E=1-289.
DR PDB; 6MUT; EM; 3.10 A; E=1-289.
DR PDB; 6MUU; EM; 3.00 A; E=1-289.
DR PDB; 6O73; X-ray; 3.00 A; B=1-289.
DR PDB; 6O74; X-ray; 2.71 A; B=1-289.
DR PDB; 6O75; X-ray; 2.60 A; B=1-289.
DR PDB; 6O78; X-ray; 2.80 A; B=1-289.
DR PDB; 6O79; X-ray; 3.00 A; B=1-289.
DR PDB; 6O7B; X-ray; 2.40 A; B=1-289.
DR PDB; 6O7D; X-ray; 2.81 A; B=1-289.
DR PDB; 6O7E; EM; 3.20 A; E=1-289.
DR PDB; 6O7H; EM; 2.90 A; E=1-289.
DR PDB; 6O7I; EM; 3.20 A; E=1-289.
DR PDBsum; 6IQW; -.
DR PDBsum; 6MUA; -.
DR PDBsum; 6MUR; -.
DR PDBsum; 6MUS; -.
DR PDBsum; 6MUT; -.
DR PDBsum; 6MUU; -.
DR PDBsum; 6O73; -.
DR PDBsum; 6O74; -.
DR PDBsum; 6O75; -.
DR PDBsum; 6O78; -.
DR PDBsum; 6O79; -.
DR PDBsum; 6O7B; -.
DR PDBsum; 6O7D; -.
DR PDBsum; 6O7E; -.
DR PDBsum; 6O7H; -.
DR PDBsum; 6O7I; -.
DR AlphaFoldDB; B6YWC1; -.
DR SMR; B6YWC1; -.
DR DIP; DIP-61405N; -.
DR IntAct; B6YWC1; 1.
DR STRING; 523850.TON_0896; -.
DR EnsemblBacteria; ACJ16384; ACJ16384; TON_0896.
DR GeneID; 7017199; -.
DR KEGG; ton:TON_0896; -.
DR PATRIC; fig|523850.10.peg.904; -.
DR eggNOG; arCOG03222; Archaea.
DR HOGENOM; CLU_062371_0_1_2; -.
DR OMA; SAYFIVE; -.
DR OrthoDB; 69746at2157; -.
DR Proteomes; UP000002727; Chromosome.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0051607; P:defense response to virus; IEA:UniProtKB-KW.
DR InterPro; IPR005510; Csm4.
DR TIGRFAMs; TIGR01903; cas5_csm4; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Antiviral defense; RNA-binding.
FT CHAIN 1..289
FT /note="CRISPR system Cms protein Csm4"
FT /id="PRO_0000446122"
FT STRAND 3..13
FT /evidence="ECO:0007829|PDB:6O7B"
FT STRAND 15..17
FT /evidence="ECO:0007829|PDB:6O78"
FT HELIX 21..36
FT /evidence="ECO:0007829|PDB:6O7B"
FT HELIX 38..49
FT /evidence="ECO:0007829|PDB:6O7B"
FT STRAND 58..61
FT /evidence="ECO:0007829|PDB:6O7B"
FT STRAND 64..68
FT /evidence="ECO:0007829|PDB:6O7B"
FT HELIX 71..73
FT /evidence="ECO:0007829|PDB:6O7B"
FT TURN 74..76
FT /evidence="ECO:0007829|PDB:6O7B"
FT TURN 77..79
FT /evidence="ECO:0007829|PDB:6O78"
FT TURN 81..83
FT /evidence="ECO:0007829|PDB:6O7H"
FT TURN 86..88
FT /evidence="ECO:0007829|PDB:6O7H"
FT HELIX 89..96
FT /evidence="ECO:0007829|PDB:6O7B"
FT STRAND 101..103
FT /evidence="ECO:0007829|PDB:6O7B"
FT HELIX 104..111
FT /evidence="ECO:0007829|PDB:6O7B"
FT STRAND 123..133
FT /evidence="ECO:0007829|PDB:6O7B"
FT TURN 136..138
FT /evidence="ECO:0007829|PDB:6O7H"
FT STRAND 142..151
FT /evidence="ECO:0007829|PDB:6O7B"
FT STRAND 155..163
FT /evidence="ECO:0007829|PDB:6O7B"
FT HELIX 165..170
FT /evidence="ECO:0007829|PDB:6O7B"
FT HELIX 172..180
FT /evidence="ECO:0007829|PDB:6O7B"
FT STRAND 184..186
FT /evidence="ECO:0007829|PDB:6O7H"
FT HELIX 188..190
FT /evidence="ECO:0007829|PDB:6O7H"
FT TURN 191..193
FT /evidence="ECO:0007829|PDB:6MUU"
FT STRAND 195..203
FT /evidence="ECO:0007829|PDB:6O7B"
FT STRAND 212..216
FT /evidence="ECO:0007829|PDB:6O7B"
FT STRAND 225..227
FT /evidence="ECO:0007829|PDB:6O75"
FT STRAND 229..231
FT /evidence="ECO:0007829|PDB:6O7B"
FT STRAND 236..239
FT /evidence="ECO:0007829|PDB:6IQW"
FT STRAND 245..247
FT /evidence="ECO:0007829|PDB:6O7B"
FT STRAND 252..255
FT /evidence="ECO:0007829|PDB:6O7B"
FT STRAND 261..263
FT /evidence="ECO:0007829|PDB:6O7B"
FT STRAND 266..269
FT /evidence="ECO:0007829|PDB:6O7B"
FT STRAND 271..273
FT /evidence="ECO:0007829|PDB:6O7B"
FT STRAND 279..281
FT /evidence="ECO:0007829|PDB:6O7B"
SQ SEQUENCE 289 AA; 32306 MW; A7E85053E77725F1 CRC64;
MPKFIAVKLI PKGPFRDIPR ADTLFGAIGN AISAIHGQSA VEELVDAFVG GARISSAFPY
SGDTYYLPKP LSVEPALEGI LTGLDEEERY TTAKRLRKAK YLDLKNFELA LRLRPFTIPE
EIPYARVDVP RVVLDRVTQD SSIYFWEEIR FREKSGVYFL YSGPREVFDG YIAPAMRFLG
DTGIGGKSTW GAGLFEVEFH EMKIDAPGSE YSVTLSNALP TKTPVLWRLL RKGGWSFGRR
KPRMTFIAEG SIVKNDPGGM ERLELGLSHE VYVYGLTFPL GVELPEGLE
