ID   CSM5_ENTI1              Reviewed;         349 AA.
AC   E6LHV3;
DT   16-JAN-2019, integrated into UniProtKB/Swiss-Prot.
DT   08-MAR-2011, sequence version 1.
DT   25-MAY-2022, entry version 35.
DE   RecName: Full=CRISPR system Cms protein Csm5;
DE   AltName: Full=CRISPR type III A-associated protein Csm5;
GN   Name=csm5; ORFNames=HMPREF9088_1943;
OS   Enterococcus italicus (strain DSM 15952 / CCUG 50447 / LMG 22039 / TP 1.5).
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
OC   Enterococcus.
OX   NCBI_TaxID=888064;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 15952 / CCUG 50447 / LMG 22039 / TP 1.5;
RA   Muzny D., Qin X., Deng J., Jiang H., Liu Y., Qu J., Song X.-Z., Zhang L.,
RA   Thornton R., Coyle M., Francisco L., Jackson L., Javaid M., Korchina V.,
RA   Kovar C., Mata R., Mathew T., Ngo R., Nguyen L., Nguyen N., Okwuonu G.,
RA   Ongeri F., Pham C., Simmons D., Wilczek-Boney K., Hale W., Jakkamsetti A.,
RA   Pham P., Ruth R., San Lucas F., Warren J., Zhang J., Zhao Z., Zhou C.,
RA   Zhu D., Lee S., Bess C., Blankenburg K., Forbes L., Fu Q., Gubbala S.,
RA   Hirani K., Jayaseelan J.C., Lara F., Munidasa M., Palculict T., Patil S.,
RA   Pu L.-L., Saada N., Tang L., Weissenberger G., Zhu Y., Hemphill L.,
RA   Shang Y., Youmans B., Ayvaz T., Ross M., Santibanez J., Aqrawi P.,
RA   Gross S., Joshi V., Fowler G., Nazareth L., Reid J., Worley K.,
RA   Petrosino J., Highlander S., Gibbs R.;
RL   Submitted (DEC-2010) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   FUNCTION IN PHAGE RESISTANCE, AND SUBUNIT.
RC   STRAIN=DSM 15952 / CCUG 50447 / LMG 22039 / TP 1.5;
RX   PubMed=28722012; DOI=10.1038/nature23467;
RA   Niewoehner O., Garcia-Doval C., Rostoel J.T., Berk C., Schwede F.,
RA   Bigler L., Hall J., Marraffini L.A., Jinek M.;
RT   "Type III CRISPR-Cas systems produce cyclic oligoadenylate second
RT   messengers.";
RL   Nature 548:543-548(2017).
CC   -!- FUNCTION: CRISPR (clustered regularly interspaced short palindromic
CC       repeat) is an adaptive immune system that provides protection against
CC       mobile genetic elements (viruses, transposable elements and conjugative
CC       plasmids). CRISPR clusters contain spacers, sequences complementary to
CC       antecedent mobile elements, and target invading nucleic acids. CRISPR
CC       clusters are transcribed and processed into CRISPR RNA (crRNA). The
CC       type III-A Csm effector complex binds crRNA and acts as a crRNA-guided
CC       RNase, DNase and cyclic oligoadenylate synthase; binding of target RNA
CC       cognate to the crRNA is required for all activities. In a heterologous
CC       host the appropriately targeted Csm effector complex prevents growth of
CC       dsDNA phage phiNM1-gamma6. {ECO:0000269|PubMed:28722012}.
CC   -!- FUNCTION: This subunit might be involved in maturation of a crRNA
CC       intermediate to its mature form. {ECO:0000250|UniProtKB:A0A0A7HF79}.
CC   -!- SUBUNIT: Part of the Csm effector complex that includes Cas10, Csm2,
CC       Csm3, Csm4 and Csm5. {ECO:0000269|PubMed:28722012}.
CC   -!- MISCELLANEOUS: Encoded in a type III-A CRISPR locus.
CC       {ECO:0000269|PubMed:28722012}.
CC   -!- SIMILARITY: Belongs to the CRISPR-associated Csm5 family.
CC       {ECO:0000305}.
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DR   EMBL; AEPV01000074; EFU73213.1; -; Genomic_DNA.
DR   RefSeq; WP_007208954.1; NZ_JXKT01000007.1.
DR   AlphaFoldDB; E6LHV3; -.
DR   SMR; E6LHV3; -.
DR   STRING; 888064.HMPREF9088_1943; -.
DR   EnsemblBacteria; EFU73213; EFU73213; HMPREF9088_1943.
DR   eggNOG; COG1332; Bacteria.
DR   HOGENOM; CLU_036878_0_0_9; -.
DR   OMA; LAPIHIG; -.
DR   Proteomes; UP000010296; Unassembled WGS sequence.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0051607; P:defense response to virus; IEA:UniProtKB-KW.
DR   InterPro; IPR010173; CRISPR-assoc_Csm5.
DR   InterPro; IPR005537; RAMP_III_fam.
DR   PANTHER; PTHR38007; PTHR38007; 1.
DR   Pfam; PF03787; RAMPs; 1.
DR   TIGRFAMs; TIGR01899; cas_TM1807_csm5; 1.
PE   1: Evidence at protein level;
KW   Antiviral defense; Reference proteome; RNA-binding.
FT   CHAIN           1..349
FT                   /note="CRISPR system Cms protein Csm5"
FT                   /id="PRO_0000446123"
SQ   SEQUENCE   349 AA;  39958 MW;  8C7DA91FCCCE6FA4 CRC64;
     MIEKVYQVKL KVYGPVHIGS GKIIRKQEYI YDRRKSLAHI VDGPNLVKFL NKKGKFTAYL
     QYLNTTKERA DLYTFLRQEQ IDTNDWKTFV LYTERVNQGK IDMKDHNPYS RTSTNRRQVD
     KGMNDLHLFV RDGRGDLYIP GSSLKGALRT VLEGANQSAE AFHSLSISDS LPIDPKNLAI
     YQKIDINKEL KPMPLYRECV NVGTTVEFTM KINSDDWTIE KIEKQIQQAY LQYWNKWFVG
     MVTTPGGKAF IKGGGLPSVL HAKHRPTVLF LGGGTGFPSK TTHYLQKPKE QAQKDIFAIL
     QRRFRNVYGK MATVPKNVPM VLKGTVNDST NKWYQQGVCL LEFQPIGEA