ID   CSM5_MYCTU              Reviewed;         375 AA.
AC   P9WJF5; F2GLB5; L0TCC2; P71633; Q7D6I5;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2014, sequence version 1.
DT   03-AUG-2022, entry version 39.
DE   RecName: Full=CRISPR system Cms protein Csm5;
DE   AltName: Full=CRISPR type III A-associated protein Csm5;
GN   Name=csm5; OrderedLocusNames=Rv2819c;
OS   Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83332;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=9634230; DOI=10.1038/31159;
RA   Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA   Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA   Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA   Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA   Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA   Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA   Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA   Barrell B.G.;
RT   "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT   genome sequence.";
RL   Nature 393:537-544(1998).
RN   [2]
RP   FUNCTION IN PLASMID RESISTANCE, SUBUNIT, AND DISRUPTION PHENOTYPE.
RC   STRAIN=H37Rv;
RX   PubMed=29979631; DOI=10.1096/fj.201800557rr;
RA   Wei W., Zhang S., Fleming J., Chen Y., Li Z., Fan S., Liu Y., Wang W.,
RA   Wang T., Liu Y., Ren B., Wang M., Jiao J., Chen Y., Zhou Y., Zhou Y.,
RA   Gu S., Zhang X., Wan L., Chen T., Zhou L., Chen Y., Zhang X.E., Li C.,
RA   Zhang H., Bi L.;
RT   "Mycobacterium tuberculosis type III-A CRISPR/Cas system crRNA and its
RT   maturation have atypical features.";
RL   FASEB J. 33:1496-1509(2019).
CC   -!- FUNCTION: CRISPR (clustered regularly interspaced short palindromic
CC       repeat) is an adaptive immune system that provides protection against
CC       mobile genetic elements (viruses, transposable elements and conjugative
CC       plasmids). CRISPR clusters contain spacers, sequences complementary to
CC       antecedent mobile elements, and target invading nucleic acids. CRISPR
CC       clusters are transcribed and processed into CRISPR RNA (crRNA). The
CC       type III-A Csm effector complex binds crRNA and acts as a crRNA-guided
CC       RNase, DNase and cyclic oligoadenylate synthase; binding of target RNA
CC       cognate to the crRNA is required for all activities (Probable). This
CC       CRISPR-Cas system protects bacteria against transformation with
CC       plasmids containing DNA homologous to its spacer regions
CC       (PubMed:29979631). {ECO:0000269|PubMed:29979631,
CC       ECO:0000305|PubMed:29979631}.
CC   -!- FUNCTION: This subunit might be involved in maturation of a crRNA
CC       intermediate to its mature form. {ECO:0000250|UniProtKB:A0A0A7HF79}.
CC   -!- SUBUNIT: Part of the Csm effector complex that includes Cas10, Csm2,
CC       Csm3, Csm4 and Csm5. {ECO:0000269|PubMed:29979631}.
CC   -!- DISRUPTION PHENOTYPE: Deletion of the entire CRISPR-Cas locus (cas6 to
CC       cas2, Rv2824c to Rv2816c) decreases resistance to plasmids encoding
CC       spacer elements about 6-fold. {ECO:0000269|PubMed:29979631}.
CC   -!- MISCELLANEOUS: Encoded in a type III-A CRISPR locus.
CC       {ECO:0000305|PubMed:29979631}.
CC   -!- SIMILARITY: Belongs to the CRISPR-associated Csm5 family.
CC       {ECO:0000305}.
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DR   EMBL; AL123456; CCP45619.1; -; Genomic_DNA.
DR   PIR; F70691; F70691.
DR   RefSeq; NP_217335.1; NC_000962.3.
DR   RefSeq; WP_003414285.1; NZ_NVQJ01000006.1.
DR   AlphaFoldDB; P9WJF5; -.
DR   SMR; P9WJF5; -.
DR   STRING; 83332.Rv2819c; -.
DR   PaxDb; P9WJF5; -.
DR   DNASU; 888514; -.
DR   GeneID; 888514; -.
DR   KEGG; mtu:Rv2819c; -.
DR   TubercuList; Rv2819c; -.
DR   eggNOG; COG1332; Bacteria.
DR   OMA; LAPIHIG; -.
DR   Proteomes; UP000001584; Chromosome.
DR   GO; GO:0005829; C:cytosol; HDA:MTBBASE.
DR   GO; GO:0005886; C:plasma membrane; HDA:MTBBASE.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0051607; P:defense response to virus; IEA:UniProtKB-KW.
DR   InterPro; IPR010173; CRISPR-assoc_Csm5.
DR   InterPro; IPR005537; RAMP_III_fam.
DR   PANTHER; PTHR38007; PTHR38007; 1.
DR   Pfam; PF03787; RAMPs; 1.
DR   TIGRFAMs; TIGR01899; cas_TM1807_csm5; 1.
PE   1: Evidence at protein level;
KW   Antiviral defense; Reference proteome; RNA-binding.
FT   CHAIN           1..375
FT                   /note="CRISPR system Cms protein Csm5"
FT                   /id="PRO_0000418228"
FT   REGION          167..195
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        167..190
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   375 AA;  42340 MW;  27B0215415EC0C25 CRC64;
     MNTYLKPFEL TLRCLGPVFI GSGEKRTSKE YHVEGDRVYF PDMELLYADI PAHKRKSFEA
     FVMNTDGAQA TAPLKEWVEP NAVKLDPAKH RGYEVKIGSI EPRRASRGRG GRMTRKKLTL
     NEIHAFIKDP LGRPYVPGST VKGMLRSIYL QSLVHKRTAQ PVRVPGHQTR EHRQYGERFE
     RKELRKSGRP NTRPQDAVND LFQAIRVTDS PALRTSDLLI CQKMDMNVHG KPDGLPLFRE
     CLAPGTSISH RVVVDTSPTA RGGWREGERF LETLAETAAS VNQARYAEYR AMYPGVNAIV
     GPIVYLGGGA GYRSKTFVTD QDDMAKVLDA QFGKVVKHVD KTRELRVSPL VLKRTKIDNI
     CYEMGQCELS IRRAE