CSN3_MOUSE
ID CSN3_MOUSE Reviewed; 423 AA.
AC O88543;
DT 23-NOV-2004, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 3.
DT 03-AUG-2022, entry version 160.
DE RecName: Full=COP9 signalosome complex subunit 3;
DE Short=SGN3;
DE Short=Signalosome subunit 3;
DE AltName: Full=JAB1-containing signalosome subunit 3;
GN Name=Cops3; Synonyms=Csn3;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND IDENTIFICATION IN THE CSN COMPLEX.
RC STRAIN=C57BL/6J;
RX PubMed=9707402; DOI=10.1016/s0960-9822(07)00372-7;
RA Wei N., Tsuge T., Serino G., Dohmae N., Takio K., Matsui M., Deng X.-W.;
RT "The COP9 complex is conserved between plants and mammals and is related to
RT the 26S proteasome regulatory complex.";
RL Curr. Biol. 8:919-922(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP FUNCTION, TISSUE SPECIFICITY, AND DISRUPTION PHENOTYPE.
RX PubMed=12972600; DOI=10.1128/mcb.23.19.6798-6808.2003;
RA Yan J., Walz K., Nakamura H., Carattini-Rivera S., Zhao Q., Vogel H.,
RA Wei N., Justice M.J., Bradley A., Lupski J.R.;
RT "COP9 signalosome subunit 3 is essential for maintenance of cell
RT proliferation in the mouse embryonic epiblast.";
RL Mol. Cell. Biol. 23:6798-6808(2003).
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-407 AND SER-410, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, Pancreas,
RC Spleen, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Component of the COP9 signalosome complex (CSN), a complex
CC involved in various cellular and developmental processes (By
CC similarity). The CSN complex is an essential regulator of the ubiquitin
CC (Ubl) conjugation pathway by mediating the deneddylation of the cullin
CC subunits of SCF-type E3 ligase complexes, leading to decrease the Ubl
CC ligase activity of SCF-type complexes such as SCF, CSA or DDB2 (By
CC similarity). The complex is also involved in phosphorylation of
CC p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8/ICSBP,
CC possibly via its association with CK2 and PKD kinases (By similarity).
CC CSN-dependent phosphorylation of TP53 and JUN promotes and protects
CC degradation by the Ubl system, respectively (By similarity). Essential
CC to maintain the survival of epiblast cells and thus the development of
CC the postimplantation embryo (PubMed:12972600).
CC {ECO:0000250|UniProtKB:Q9UNS2, ECO:0000269|PubMed:12972600}.
CC -!- SUBUNIT: Component of the CSN complex, composed of COPS1/GPS1, COPS2,
CC COPS3, COPS4, COPS5, COPS6, COPS7 (COPS7A or COPS7B), COPS8 and COPS9
CC (PubMed:9707402). In the complex, it probably interacts directly with
CC COPS1, COPS4, COPS8 and COPS9 (By similarity). Interacts with CK2 and
CC PKD (By similarity). Interacts with the translation initiation factor
CC EIF3S6 and IKBKG (By similarity). Interacts with ERCC6 (By similarity).
CC {ECO:0000250|UniProtKB:Q9UNS2, ECO:0000269|PubMed:9707402}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:Q9UNS2}. Nucleus
CC {ECO:0000250|UniProtKB:Q9UNS2}.
CC -!- TISSUE SPECIFICITY: Widely expressed. {ECO:0000269|PubMed:12972600}.
CC -!- DISRUPTION PHENOTYPE: Embryos arrest after 5.5 dpc and resorb by 8.5
CC dpc mainly due to increased cell death. {ECO:0000269|PubMed:12972600}.
CC -!- SIMILARITY: Belongs to the CSN3 family. {ECO:0000305}.
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DR EMBL; AF071313; AAC33900.1; -; mRNA.
DR EMBL; BC068179; AAH68179.1; -; mRNA.
DR CCDS; CCDS24778.1; -.
DR RefSeq; NP_036121.1; NM_011991.1.
DR AlphaFoldDB; O88543; -.
DR SMR; O88543; -.
DR BioGRID; 205027; 39.
DR CORUM; O88543; -.
DR IntAct; O88543; 2.
DR MINT; O88543; -.
DR STRING; 10090.ENSMUSP00000019517; -.
DR iPTMnet; O88543; -.
DR PhosphoSitePlus; O88543; -.
DR SwissPalm; O88543; -.
DR EPD; O88543; -.
DR jPOST; O88543; -.
DR PaxDb; O88543; -.
DR PeptideAtlas; O88543; -.
DR PRIDE; O88543; -.
DR ProteomicsDB; 285210; -.
DR Antibodypedia; 13330; 376 antibodies from 39 providers.
DR DNASU; 26572; -.
DR Ensembl; ENSMUST00000019517; ENSMUSP00000019517; ENSMUSG00000019373.
DR GeneID; 26572; -.
DR KEGG; mmu:26572; -.
DR UCSC; uc007jfa.1; mouse.
DR CTD; 8533; -.
DR MGI; MGI:1349409; Cops3.
DR VEuPathDB; HostDB:ENSMUSG00000019373; -.
DR eggNOG; KOG2582; Eukaryota.
DR GeneTree; ENSGT00940000153653; -.
DR HOGENOM; CLU_028825_0_1_1; -.
DR InParanoid; O88543; -.
DR OMA; NHYHDLV; -.
DR OrthoDB; 929822at2759; -.
DR PhylomeDB; O88543; -.
DR TreeFam; TF101146; -.
DR Reactome; R-MMU-5696394; DNA Damage Recognition in GG-NER.
DR Reactome; R-MMU-6781823; Formation of TC-NER Pre-Incision Complex.
DR Reactome; R-MMU-8856825; Cargo recognition for clathrin-mediated endocytosis.
DR Reactome; R-MMU-8951664; Neddylation.
DR BioGRID-ORCS; 26572; 28 hits in 72 CRISPR screens.
DR ChiTaRS; Cops3; mouse.
DR PRO; PR:O88543; -.
DR Proteomes; UP000000589; Chromosome 11.
DR RNAct; O88543; protein.
DR Bgee; ENSMUSG00000019373; Expressed in spermatocyte and 280 other tissues.
DR ExpressionAtlas; O88543; baseline and differential.
DR Genevisible; O88543; MM.
DR GO; GO:0008180; C:COP9 signalosome; IDA:MGI.
DR GO; GO:0005737; C:cytoplasm; ISO:MGI.
DR GO; GO:0005829; C:cytosol; ISO:MGI.
DR GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR GO; GO:0005634; C:nucleus; ISO:MGI.
DR GO; GO:0048471; C:perinuclear region of cytoplasm; ISO:MGI.
DR GO; GO:0001701; P:in utero embryonic development; IMP:MGI.
DR GO; GO:0000338; P:protein deneddylation; ISO:MGI.
DR GO; GO:1902162; P:regulation of DNA damage response, signal transduction by p53 class mediator resulting in transcription of p21 class mediator; ISO:MGI.
DR GO; GO:0006511; P:ubiquitin-dependent protein catabolic process; IBA:GO_Central.
DR InterPro; IPR037753; CSN3.
DR InterPro; IPR000717; PCI_dom.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR PANTHER; PTHR10758:SF1; PTHR10758:SF1; 1.
DR Pfam; PF01399; PCI; 1.
DR SMART; SM00088; PINT; 1.
DR SUPFAM; SSF46785; SSF46785; 1.
DR PROSITE; PS50250; PCI; 1.
PE 1: Evidence at protein level;
KW Acetylation; Cytoplasm; Nucleus; Phosphoprotein; Reference proteome;
KW Signalosome.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000250|UniProtKB:Q9UNS2"
FT CHAIN 2..423
FT /note="COP9 signalosome complex subunit 3"
FT /id="PRO_0000120979"
FT DOMAIN 197..365
FT /note="PCI"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01185"
FT REGION 402..423
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 2
FT /note="N-acetylalanine"
FT /evidence="ECO:0000250|UniProtKB:Q9UNS2"
FT MOD_RES 407
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 410
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 423
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9UNS2"
SQ SEQUENCE 423 AA; 47832 MW; 2FDCCDB98A5168FB CRC64;
MASALEQFVN SVRQLSAQGQ MTQLCELINK SGELLAKNLS HLDTVLGALD VQEHSLGVLA
VLFVKFSMPS VPDFETLFSQ VQLFISTCNG EHIRYATDTF AGLCHQLTNA LVERKQPLRG
IGILKQAIDK MQMNTNQLTS VHADLCQLCL LAKCFKPALP YLDVDMMDIC KENGAYDAKH
FLCYYYYGGM IYTGLKNFER ALYFYEQAIT TPAMAVSHIM LESYKKYILV SLILLGKVQQ
LPKYTSQIVG RFIKPLSNAY HELAQVYSTN NPSELRNLVS KHSETFTRDN NMGLVKQCLS
SLYKKNIQRL TKTFLTLSLQ DMASRVQLSG PQEAEKYVLH MIEDGEIFAS INQKDGMVSF
HDNPEKYNNP AMLHNIDQEM LKCIELDERL KAMDQEITVN PQFVQKSMGS QEDDSGNKPS
SYS
