CSN8_DROME
ID CSN8_DROME Reviewed; 182 AA.
AC Q7KTH8; Q0E8L3;
DT 23-NOV-2004, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 121.
DE RecName: Full=COP9 signalosome complex subunit 8;
DE Short=Dch8;
DE Short=Signalosome subunit 8;
GN Name=CSN8; ORFNames=CG13383;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Embryo;
RA Stapleton M., Carlson J.W., Chavez C., Frise E., George R.A., Pacleb J.M.,
RA Park S., Wan K.H., Yu C., Rubin G.M., Celniker S.E.;
RL Submitted (FEB-2004) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP IDENTIFICATION.
RX PubMed=10531038; DOI=10.1016/s0960-9822(00)80023-8;
RA Freilich S., Oron E., Kapp Y., Nevo-Caspi Y., Orgad S., Segal D.,
RA Chamovitz D.A.;
RT "The COP9 signalosome is essential for development of Drosophila
RT melanogaster.";
RL Curr. Biol. 9:1187-1190(1999).
RN [5]
RP FUNCTION OF CSN COMPLEX.
RX PubMed=12737805; DOI=10.1016/s1534-5807(03)00121-7;
RA Doronkin S., Djagaeva I., Beckendorf S.K.;
RT "The COP9 signalosome promotes degradation of Cyclin E during early
RT Drosophila oogenesis.";
RL Dev. Cell 4:699-710(2003).
RN [6]
RP FUNCTION, SUBUNIT, AND DEVELOPMENTAL STAGE.
RX PubMed=18298797; DOI=10.1111/j.1365-2443.2008.01164.x;
RA Oren-Giladi P., Krieger O., Edgar B.A., Chamovitz D.A., Segal D.;
RT "Cop9 signalosome subunit 8 (CSN8) is essential for Drosophila
RT development.";
RL Genes Cells 13:221-231(2008).
CC -!- FUNCTION: Probable component of the COP9 signalosome complex (CSN), a
CC complex involved in various cellular and developmental processes. The
CC CSN complex is an essential regulator of the ubiquitin (Ubl)
CC conjugation pathway by mediating the deneddylation of the cullin
CC subunits of the SCF-type E3 ligase complexes, leading to decrease the
CC Ubl ligase activity of SCF. The CSN complex plays an essential role in
CC oogenesis and embryogenesis and is required for proper photoreceptor R
CC cell differentiation and promote lamina glial cell migration or axon
CC targeting. It also promotes Ubl-dependent degradation of cyclin E
CC (CycE) during early oogenesis. {ECO:0000269|PubMed:12737805,
CC ECO:0000269|PubMed:18298797}.
CC -!- SUBUNIT: Essential component of the CSN complex, probably composed of
CC CSN1b, alien/CSN2, CSN3, CSN4, CSN5, CSN6, CSN7 and CSN8.
CC {ECO:0000269|PubMed:18298797}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}. Nucleus {ECO:0000305}.
CC -!- DEVELOPMENTAL STAGE: Expressed maternally and zygotically throughout
CC development. {ECO:0000269|PubMed:18298797}.
CC -!- DOMAIN: The PCI domain is necessary and sufficient for interactions
CC with other CSN subunits of the complex.
CC -!- SIMILARITY: Belongs to the CSN8 family. {ECO:0000305}.
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DR EMBL; AE014134; AAN10662.2; -; Genomic_DNA.
DR EMBL; BT011518; AAS15654.1; -; mRNA.
DR RefSeq; NP_723378.2; NM_164809.5.
DR AlphaFoldDB; Q7KTH8; -.
DR SMR; Q7KTH8; -.
DR BioGRID; 302275; 15.
DR IntAct; Q7KTH8; 1.
DR STRING; 7227.FBpp0079262; -.
DR PaxDb; Q7KTH8; -.
DR PRIDE; Q7KTH8; -.
DR DNASU; 49077; -.
DR EnsemblMetazoa; FBtr0079646; FBpp0079262; FBgn0261437.
DR GeneID; 49077; -.
DR KEGG; dme:Dmel_CG42522; -.
DR CTD; 49077; -.
DR FlyBase; FBgn0261437; CSN8.
DR VEuPathDB; VectorBase:FBgn0261437; -.
DR eggNOG; KOG4414; Eukaryota.
DR HOGENOM; CLU_098091_1_1_1; -.
DR InParanoid; Q7KTH8; -.
DR OMA; DAKLLWM; -.
DR OrthoDB; 1577916at2759; -.
DR PhylomeDB; Q7KTH8; -.
DR Reactome; R-DME-5696394; DNA Damage Recognition in GG-NER.
DR Reactome; R-DME-6781823; Formation of TC-NER Pre-Incision Complex.
DR Reactome; R-DME-8856825; Cargo recognition for clathrin-mediated endocytosis.
DR Reactome; R-DME-8951664; Neddylation.
DR SignaLink; Q7KTH8; -.
DR BioGRID-ORCS; 49077; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 49077; -.
DR PRO; PR:Q7KTH8; -.
DR Proteomes; UP000000803; Chromosome 2L.
DR Bgee; FBgn0261437; Expressed in secondary oocyte and 26 other tissues.
DR Genevisible; Q7KTH8; DM.
DR GO; GO:0008180; C:COP9 signalosome; IDA:UniProtKB.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0001745; P:compound eye morphogenesis; IMP:FlyBase.
DR GO; GO:0010387; P:COP9 signalosome assembly; IMP:FlyBase.
DR GO; GO:0042742; P:defense response to bacterium; IMP:FlyBase.
DR GO; GO:0007476; P:imaginal disc-derived wing morphogenesis; IMP:FlyBase.
DR GO; GO:0048477; P:oogenesis; IMP:UniProtKB.
DR GO; GO:0042461; P:photoreceptor cell development; IMP:UniProtKB.
DR GO; GO:0000338; P:protein deneddylation; IMP:FlyBase.
DR GO; GO:0050821; P:protein stabilization; IMP:FlyBase.
DR GO; GO:0060625; P:regulation of protein deneddylation; IMP:UniProtKB.
DR InterPro; IPR033205; COP9_CSN8.
DR InterPro; IPR033464; CSN8_PSD8_EIF3K.
DR InterPro; IPR000717; PCI_dom.
DR PANTHER; PTHR13339; PTHR13339; 1.
DR Pfam; PF10075; CSN8_PSD8_EIF3K; 1.
DR PROSITE; PS50250; PCI; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Developmental protein; Differentiation; Nucleus; Oogenesis;
KW Reference proteome; Signalosome.
FT CHAIN 1..182
FT /note="COP9 signalosome complex subunit 8"
FT /id="PRO_0000121014"
FT DOMAIN 1..167
FT /note="PCI"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01185"
SQ SEQUENCE 182 AA; 21431 MW; F04592E802DD8405 CRC64;
MHLNKYSEVV ERLENEEFEQ VELGAEVYQQ LLAIYLYQNK LADAKLLWMR VPANLRDDKE
LIQLNLLNIA LQNNNYADFF KHIKYEWSER VKSPVEDLLN KQREELFKLM GSAYMSIYQH
NLLELSLMSE DELKHACAAL NWTEELDGDR VILKPKVQEA PPARGNDDQL LKLTEFVTFL
EN
