ID   CSNB_ASPOZ              Reviewed;         240 AA.
AC   Q8NK76;
DT   09-JUL-2014, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2002, sequence version 1.
DT   25-MAY-2022, entry version 38.
DE   RecName: Full=Endo-chitosanase B;
DE            EC=3.2.1.132;
DE   Flags: Precursor;
GN   Name=csnB;
OS   Aspergillus oryzae (Yellow koji mold).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC   Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus;
OC   Aspergillus subgen. Circumdati.
OX   NCBI_TaxID=5062;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=IAM2660;
RA   Miyazawa T., Zhang X., Shimosaka M., Okazaki M.;
RT   "Cloning of a chitosanase (csnB) gene from Aspergillus oryzae strain
RT   IAM2660.";
RL   Submitted (AUG-2002) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Chitosanase catalyzing the endo-type cleavage of chitosan,
CC       the deacylated form of chitin. Chitosanase may be crucial in the
CC       degradation of the deacetylated portion of chitin in the fungal cell
CC       wall. Chitoolisaccharides produced by the hydrolysis of partially N-
CC       acetylated chitosan are known to have many biological activities,
CC       including antibacterial activity, immune-enhancing effects, and
CC       elicitor activity (By similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Endohydrolysis of beta-(1->4)-linkages between D-glucosamine
CC         residues in a partly acetylated chitosan.; EC=3.2.1.132;
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 75 family. {ECO:0000305}.
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DR   EMBL; AB090327; BAC10610.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q8NK76; -.
DR   STRING; 5062.CADAORAP00004748; -.
DR   CAZy; GH75; Glycoside Hydrolase Family 75.
DR   VEuPathDB; FungiDB:AO090011000027; -.
DR   eggNOG; ENOG502S39Y; Eukaryota.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0016977; F:chitosanase activity; IEA:UniProtKB-EC.
DR   GO; GO:0000272; P:polysaccharide catabolic process; IEA:UniProtKB-KW.
DR   InterPro; IPR009939; Chitosanase_fungal.
DR   PANTHER; PTHR42061; PTHR42061; 1.
DR   Pfam; PF07335; Glyco_hydro_75; 1.
PE   3: Inferred from homology;
KW   Carbohydrate metabolism; Glycoprotein; Glycosidase; Hydrolase;
KW   Polysaccharide degradation; Secreted; Signal.
FT   SIGNAL          1..17
FT                   /evidence="ECO:0000255"
FT   CHAIN           18..240
FT                   /note="Endo-chitosanase B"
FT                   /id="PRO_0000429637"
FT   CARBOHYD        86
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   240 AA;  25841 MW;  20D5EACBE49C21F6 CRC64;
     MRLSEILAVA LVTGATAYDL PDNLKQIYEK HKGKCSKVYQ KGFTNGGHSD GKSFEYCGDI
     EGAIFMHSSA KGGQYTNMDV DCDGANNSAG KCSNDPSGQG VTAFKDEVKK FGIPDLDANL
     HPYIVFGNEE HSPQFKPQKY GMEPLSVMAV VCNGKLHYGI WGDTNGGTST GEASLSMAEL
     CFPEEKPDGD HGHDDNDVLY IGFTGKDAVL ERVPLEGKKT EDFEDSIKSI GDKLVAGLKA