ID   CSN_ASPFU               Reviewed;         242 AA.
AC   Q4WB37;
DT   09-JUL-2014, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2005, sequence version 1.
DT   25-MAY-2022, entry version 69.
DE   RecName: Full=Endo-chitosanase;
DE            EC=3.2.1.132;
DE   Flags: Precursor;
GN   Name=csn; ORFNames=AFUA_8G00930;
OS   Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC
OS   A1100) (Aspergillus fumigatus).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC   Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus;
OC   Aspergillus subgen. Fumigati.
OX   NCBI_TaxID=330879;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100;
RX   PubMed=16372009; DOI=10.1038/nature04332;
RA   Nierman W.C., Pain A., Anderson M.J., Wortman J.R., Kim H.S., Arroyo J.,
RA   Berriman M., Abe K., Archer D.B., Bermejo C., Bennett J.W., Bowyer P.,
RA   Chen D., Collins M., Coulsen R., Davies R., Dyer P.S., Farman M.L.,
RA   Fedorova N., Fedorova N.D., Feldblyum T.V., Fischer R., Fosker N.,
RA   Fraser A., Garcia J.L., Garcia M.J., Goble A., Goldman G.H., Gomi K.,
RA   Griffith-Jones S., Gwilliam R., Haas B.J., Haas H., Harris D.E.,
RA   Horiuchi H., Huang J., Humphray S., Jimenez J., Keller N., Khouri H.,
RA   Kitamoto K., Kobayashi T., Konzack S., Kulkarni R., Kumagai T., Lafton A.,
RA   Latge J.-P., Li W., Lord A., Lu C., Majoros W.H., May G.S., Miller B.L.,
RA   Mohamoud Y., Molina M., Monod M., Mouyna I., Mulligan S., Murphy L.D.,
RA   O'Neil S., Paulsen I., Penalva M.A., Pertea M., Price C., Pritchard B.L.,
RA   Quail M.A., Rabbinowitsch E., Rawlins N., Rajandream M.A., Reichard U.,
RA   Renauld H., Robson G.D., Rodriguez de Cordoba S., Rodriguez-Pena J.M.,
RA   Ronning C.M., Rutter S., Salzberg S.L., Sanchez M., Sanchez-Ferrero J.C.,
RA   Saunders D., Seeger K., Squares R., Squares S., Takeuchi M., Tekaia F.,
RA   Turner G., Vazquez de Aldana C.R., Weidman J., White O., Woodward J.R.,
RA   Yu J.-H., Fraser C.M., Galagan J.E., Asai K., Machida M., Hall N.,
RA   Barrell B.G., Denning D.W.;
RT   "Genomic sequence of the pathogenic and allergenic filamentous fungus
RT   Aspergillus fumigatus.";
RL   Nature 438:1151-1156(2005).
CC   -!- FUNCTION: Chitosanase catalyzing the endo-type cleavage of chitosan,
CC       the deacylated form of chitin. Chitosanase may be crucial in the
CC       degradation of the deacetylated portion of chitin in the fungal cell
CC       wall. Chitoolisaccharides produced by the hydrolysis of partially N-
CC       acetylated chitosan are known to have many biological activities,
CC       including antibacterial activity, immune-enhancing effects, and
CC       elicitor activity. The chitosans with higher degrees of deacetylation
CC       were shown to be the better substrates. Chitodimer, chitotrimer, and
CC       chitotetramer are the major products but monoacetyl chitodimer,
CC       monoacetyl chitotrimer, and monoacetyl chitotetramer are also produced
CC       (By similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Endohydrolysis of beta-(1->4)-linkages between D-glucosamine
CC         residues in a partly acetylated chitosan.; EC=3.2.1.132;
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
CC   -!- ALLERGEN: Causes an allergic reaction in human. Binds to IgE (By
CC       similarity). {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 75 family. {ECO:0000305}.
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DR   EMBL; AAHF01000014; EAL85075.1; -; Genomic_DNA.
DR   RefSeq; XP_747113.1; XM_742020.1.
DR   AlphaFoldDB; Q4WB37; -.
DR   STRING; 746128.CADAFUBP00008342; -.
DR   EnsemblFungi; EAL85075; EAL85075; AFUA_8G00930.
DR   GeneID; 3504463; -.
DR   KEGG; afm:AFUA_8G00930; -.
DR   VEuPathDB; FungiDB:Afu8g00930; -.
DR   eggNOG; ENOG502S39Y; Eukaryota.
DR   HOGENOM; CLU_046555_0_0_1; -.
DR   InParanoid; Q4WB37; -.
DR   OMA; SGDNGHD; -.
DR   OrthoDB; 1307217at2759; -.
DR   Proteomes; UP000002530; Chromosome 8.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0016977; F:chitosanase activity; IEA:UniProtKB-EC.
DR   GO; GO:0000272; P:polysaccharide catabolic process; IEA:UniProtKB-KW.
DR   InterPro; IPR009939; Chitosanase_fungal.
DR   PANTHER; PTHR42061; PTHR42061; 1.
DR   Pfam; PF07335; Glyco_hydro_75; 1.
PE   3: Inferred from homology;
KW   Allergen; Carbohydrate metabolism; Glycoprotein; Glycosidase; Hydrolase;
KW   Polysaccharide degradation; Reference proteome; Secreted; Signal.
FT   SIGNAL          1..18
FT                   /evidence="ECO:0000255"
FT   CHAIN           19..242
FT                   /note="Endo-chitosanase"
FT                   /id="PRO_0000429634"
FT   CARBOHYD        87
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   242 AA;  25331 MW;  879AEFE458BD334E CRC64;
     MHFLATAAVL AGVGLASAYT VPANLQQIYN KHKTGTCQNK LQDGFSDGIS GSRSFAYCGD
     IEGAIFLHSS ANGGQYVNMD IDCDGANNSA GDCANDPSGQ SMTAFMDTVK QYGISDLDAN
     IHPYVVFGNS GSSPTFDPQQ YGMEPLSVMA VVCNNQLFYG IWGDTNGGTS TGEASISLAK
     LCFPNDGITG DNGHGEEDVL YIGFMGQDAV PGASAAWTAR DTKTFEESIK ALGDRLVAKL
     SA