ID   CSN_ASPOZ               Reviewed;         245 AA.
AC   Q9P961;
DT   09-JUL-2014, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2000, sequence version 1.
DT   25-MAY-2022, entry version 71.
DE   RecName: Full=Endo-chitosanase;
DE            EC=3.2.1.132;
DE   Flags: Precursor;
GN   Name=csn; Synonyms=csnA;
OS   Aspergillus oryzae (Yellow koji mold).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC   Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus;
OC   Aspergillus subgen. Circumdati.
OX   NCBI_TaxID=5062;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND CATALYTIC ACTIVITY.
RC   STRAIN=IAM2660;
RX   PubMed=11388486; DOI=10.1271/bbb.65.977;
RA   Zhang X.Y., Dai A.L., Kuroiwa K., Kodaira R., Nogawa M., Shimosaka M.,
RA   Okazaki M.;
RT   "Cloning and characterization of a chitosanase gene from the koji mold
RT   Aspergillus oryzae strain IAM 2660.";
RL   Biosci. Biotechnol. Biochem. 65:977-981(2001).
CC   -!- FUNCTION: Chitosanase catalyzing the endo-type cleavage of chitosan,
CC       the deacylated form of chitin. Chitosanase may be crucial in the
CC       degradation of the deacetylated portion of chitin in the fungal cell
CC       wall. Chitoolisaccharides produced by the hydrolysis of partially N-
CC       acetylated chitosan are known to have many biological activities,
CC       including antibacterial activity, immune-enhancing effects, and
CC       elicitor activity. {ECO:0000269|PubMed:11388486}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Endohydrolysis of beta-(1->4)-linkages between D-glucosamine
CC         residues in a partly acetylated chitosan.; EC=3.2.1.132;
CC         Evidence={ECO:0000269|PubMed:11388486};
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 75 family. {ECO:0000305}.
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DR   EMBL; AB038996; BAA92250.1; -; Genomic_DNA.
DR   PIR; JC7659; JC7659.
DR   AlphaFoldDB; Q9P961; -.
DR   CAZy; GH75; Glycoside Hydrolase Family 75.
DR   CLAE; CSN75A_ASPOR; -.
DR   EnsemblFungi; BAE63840; BAE63840; AO090020000697.
DR   VEuPathDB; FungiDB:AO090020000697; -.
DR   eggNOG; ENOG502S39Y; Eukaryota.
DR   OMA; SGDNGHD; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0016977; F:chitosanase activity; IEA:UniProtKB-EC.
DR   GO; GO:0000272; P:polysaccharide catabolic process; IEA:UniProtKB-KW.
DR   InterPro; IPR009939; Chitosanase_fungal.
DR   PANTHER; PTHR42061; PTHR42061; 1.
DR   Pfam; PF07335; Glyco_hydro_75; 1.
PE   1: Evidence at protein level;
KW   Carbohydrate metabolism; Glycosidase; Hydrolase;
KW   Polysaccharide degradation; Secreted; Signal.
FT   SIGNAL          1..17
FT                   /evidence="ECO:0000255"
FT   CHAIN           18..245
FT                   /note="Endo-chitosanase"
FT                   /id="PRO_0000429635"
SQ   SEQUENCE   245 AA;  26478 MW;  085E5B342F6C6CD8 CRC64;
     MHFAGIVAIA LATGATAYDL PENLKQIYEK HKSGKCSKEL QGGYDNGHSH DGKSFSYCGD
     IPNAIYLHSS KNGGQYADMD IDCDGANRHA GKCSNDHSGQ GETRWKDEVQ KLGIDDLDAN
     IHPYVVFGNE NDDGDDPEFD PRKHGMEPLS VMAVVCNKKL FYGIWGDTNG HTATGEASLS
     MAELCFPEED PSGDSGHEPN DVLYIGFTGK EAVPGKSADW KADSTESFEE SIKELGDKLV
     AGLKA