ID   CSPA_ECO57              Reviewed;          70 AA.
AC   P0A9Y1; P15277; P37410; Q54170;
DT   13-SEP-2005, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 2.
DT   03-AUG-2022, entry version 96.
DE   RecName: Full=Cold shock protein CspA;
DE            Short=CSP-A;
DE   AltName: Full=7.4 kDa cold shock protein;
DE   AltName: Full=CS7.4;
GN   Name=cspA; OrderedLocusNames=Z4981, ECs4441;
OS   Escherichia coli O157:H7.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83334;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=O157:H7 / EDL933 / ATCC 700927 / EHEC;
RX   PubMed=11206551; DOI=10.1038/35054089;
RA   Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J.,
RA   Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G.,
RA   Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J.,
RA   Davis N.W., Lim A., Dimalanta E.T., Potamousis K., Apodaca J.,
RA   Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A.,
RA   Blattner F.R.;
RT   "Genome sequence of enterohaemorrhagic Escherichia coli O157:H7.";
RL   Nature 409:529-533(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=O157:H7 / Sakai / RIMD 0509952 / EHEC;
RX   PubMed=11258796; DOI=10.1093/dnares/8.1.11;
RA   Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K.,
RA   Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T.,
RA   Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T., Kuhara S.,
RA   Shiba T., Hattori M., Shinagawa H.;
RT   "Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and
RT   genomic comparison with a laboratory strain K-12.";
RL   DNA Res. 8:11-22(2001).
CC   -!- FUNCTION: Binds to and stimulates the transcription of the CCAAT-
CC       containing, cold-shock-inducible promoters of the H-NS and GyrA
CC       proteins. Binds also to the inverted repeat 5'-ATTGG-3' (By
CC       similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- INDUCTION: In response to low temperature. {ECO:0000250}.
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DR   EMBL; AE005174; AAG58705.1; -; Genomic_DNA.
DR   EMBL; BA000007; BAB37864.1; -; Genomic_DNA.
DR   PIR; A91184; A91184.
DR   PIR; E86030; E86030.
DR   RefSeq; NP_312468.1; NC_002695.1.
DR   RefSeq; WP_000014594.1; NZ_SWKA01000005.1.
DR   AlphaFoldDB; P0A9Y1; -.
DR   BMRB; P0A9Y1; -.
DR   SMR; P0A9Y1; -.
DR   STRING; 155864.EDL933_4821; -.
DR   EnsemblBacteria; AAG58705; AAG58705; Z4981.
DR   EnsemblBacteria; BAB37864; BAB37864; ECs_4441.
DR   GeneID; 64293021; -.
DR   GeneID; 67517040; -.
DR   GeneID; 915643; -.
DR   KEGG; ece:Z4981; -.
DR   KEGG; ecs:ECs_4441; -.
DR   PATRIC; fig|386585.9.peg.4648; -.
DR   eggNOG; COG1278; Bacteria.
DR   HOGENOM; CLU_117621_2_1_6; -.
DR   OMA; GPCANKV; -.
DR   Proteomes; UP000000558; Chromosome.
DR   Proteomes; UP000002519; Chromosome.
DR   GO; GO:0005829; C:cytosol; IEA:UniProt.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   CDD; cd04458; CSP_CDS; 1.
DR   Gene3D; 2.40.50.140; -; 1.
DR   InterPro; IPR012156; Cold_shock_CspA.
DR   InterPro; IPR011129; CSD.
DR   InterPro; IPR019844; CSD_1.
DR   InterPro; IPR002059; CSP_DNA-bd.
DR   InterPro; IPR012340; NA-bd_OB-fold.
DR   Pfam; PF00313; CSD; 1.
DR   PIRSF; PIRSF002599; Cold_shock_A; 1.
DR   PRINTS; PR00050; COLDSHOCK.
DR   SMART; SM00357; CSP; 1.
DR   SUPFAM; SSF50249; SSF50249; 1.
DR   PROSITE; PS00352; CSD_1; 1.
DR   PROSITE; PS51857; CSD_2; 1.
PE   3: Inferred from homology;
KW   Activator; Cytoplasm; DNA-binding; Reference proteome; Stress response;
KW   Transcription; Transcription regulation.
FT   INIT_MET        1
FT                   /note="Removed"
FT                   /evidence="ECO:0000250"
FT   CHAIN           2..70
FT                   /note="Cold shock protein CspA"
FT                   /id="PRO_0000100235"
FT   DOMAIN          7..67
FT                   /note="CSD"
SQ   SEQUENCE   70 AA;  7403 MW;  CED47F00BF18A49B CRC64;
     MSGKMTGIVK WFNADKGFGF ITPDDGSKDV FVHFSAIQND GYKSLDEGQK VSFTIESGAK
     GPAAGNVTSL