ID   CSPA_STAAC              Reviewed;          66 AA.
AC   Q5HG18; Q8RJZ5;
DT   28-NOV-2006, integrated into UniProtKB/Swiss-Prot.
DT   15-FEB-2005, sequence version 1.
DT   25-MAY-2022, entry version 96.
DE   RecName: Full=Cold shock protein CspA;
GN   Name=cspA; OrderedLocusNames=SACOL1437;
OS   Staphylococcus aureus (strain COL).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=93062;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=10566865; DOI=10.1089/mdr.1999.5.163;
RA   de Lencastre H., Wu S.-W., Pinho M.G., Ludovice A.M., Filipe S.,
RA   Gardete S., Sobral R., Gill S.R., Chung M., Tomasz A.;
RT   "Antibiotic resistance as a stress response: complete sequencing of a large
RT   number of chromosomal loci in Staphylococcus aureus strain COL that impact
RT   on the expression of resistance to methicillin.";
RL   Microb. Drug Resist. 5:163-175(1999).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=COL;
RX   PubMed=15774886; DOI=10.1128/jb.187.7.2426-2438.2005;
RA   Gill S.R., Fouts D.E., Archer G.L., Mongodin E.F., DeBoy R.T., Ravel J.,
RA   Paulsen I.T., Kolonay J.F., Brinkac L.M., Beanan M.J., Dodson R.J.,
RA   Daugherty S.C., Madupu R., Angiuoli S.V., Durkin A.S., Haft D.H.,
RA   Vamathevan J.J., Khouri H., Utterback T.R., Lee C., Dimitrov G., Jiang L.,
RA   Qin H., Weidman J., Tran K., Kang K.H., Hance I.R., Nelson K.E.,
RA   Fraser C.M.;
RT   "Insights on evolution of virulence and resistance from the complete genome
RT   analysis of an early methicillin-resistant Staphylococcus aureus strain and
RT   a biofilm-producing methicillin-resistant Staphylococcus epidermidis
RT   strain.";
RL   J. Bacteriol. 187:2426-2438(2005).
RN   [3]
RP   FUNCTION IN COLD STRESS RESPONSE, AND SUSCEPTIBILITY TO ANTIMICROBIAL
RP   PEPTIDE.
RX   PubMed=12874306; DOI=10.1128/iai.71.8.4304-4312.2003;
RA   Katzif S., Danavall D., Bowers S., Balthazar J.T., Shafer W.M.;
RT   "The major cold shock gene, cspA, is involved in the susceptibility of
RT   Staphylococcus aureus to an antimicrobial peptide of human cathepsin G.";
RL   Infect. Immun. 71:4304-4312(2003).
RN   [4]
RP   FUNCTION IN PIGMENT PRODUCTION.
RX   PubMed=16291691; DOI=10.1128/jb.187.23.8181-8184.2005;
RA   Katzif S., Lee E.-H., Law A.B., Tzeng Y.-L., Shafer W.M.;
RT   "CspA regulates pigment production in Staphylococcus aureus through a SigB-
RT   dependent mechanism.";
RL   J. Bacteriol. 187:8181-8184(2005).
CC   -!- FUNCTION: Involved in cold stress response and in the susceptibility to
CC       an antimicrobial peptide of human cathepsin G (CG117-136). Regulates
CC       yellowish-orange pigment production through a still unclear SigB-
CC       dependent mechanism. {ECO:0000269|PubMed:12874306,
CC       ECO:0000269|PubMed:16291691}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
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DR   EMBL; Y18632; CAC80094.1; -; Genomic_DNA.
DR   EMBL; CP000046; AAW38182.1; -; Genomic_DNA.
DR   RefSeq; WP_000809131.1; NC_002951.2.
DR   AlphaFoldDB; Q5HG18; -.
DR   SMR; Q5HG18; -.
DR   EnsemblBacteria; AAW38182; AAW38182; SACOL1437.
DR   GeneID; 66839594; -.
DR   KEGG; sac:SACOL1437; -.
DR   HOGENOM; CLU_117621_6_1_9; -.
DR   OMA; HYSTIKM; -.
DR   Proteomes; UP000000530; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR   CDD; cd04458; CSP_CDS; 1.
DR   Gene3D; 2.40.50.140; -; 1.
DR   InterPro; IPR012156; Cold_shock_CspA.
DR   InterPro; IPR011129; CSD.
DR   InterPro; IPR019844; CSD_1.
DR   InterPro; IPR002059; CSP_DNA-bd.
DR   InterPro; IPR012340; NA-bd_OB-fold.
DR   Pfam; PF00313; CSD; 1.
DR   PIRSF; PIRSF002599; Cold_shock_A; 1.
DR   PRINTS; PR00050; COLDSHOCK.
DR   SMART; SM00357; CSP; 1.
DR   SUPFAM; SSF50249; SSF50249; 1.
DR   PROSITE; PS00352; CSD_1; 1.
DR   PROSITE; PS51857; CSD_2; 1.
PE   1: Evidence at protein level;
KW   Cytoplasm.
FT   CHAIN           1..66
FT                   /note="Cold shock protein CspA"
FT                   /id="PRO_0000262541"
FT   DOMAIN          1..66
FT                   /note="CSD"
FT   CONFLICT        27
FT                   /note="F -> L (in Ref. 1; CAC80094)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   66 AA;  7321 MW;  E80AFCA652622943 CRC64;
     MKQGTVKWFN AEKGFGFIEV EGENDVFVHF SAINQDGYKS LEEGQAVEFE VVEGDRGPQA
     ANVVKL