ID   CSPC_ECOLI              Reviewed;          69 AA.
AC   P0A9Y6; O68636; P36996;
DT   13-SEP-2005, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 2.
DT   03-AUG-2022, entry version 116.
DE   RecName: Full=Cold shock-like protein CspC;
DE            Short=CSP-C;
GN   Name=cspC; Synonyms=msmB; OrderedLocusNames=b1823, JW1812;
OS   Escherichia coli (strain K12).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=TAP90 / ATCC 47037;
RX   PubMed=8022261; DOI=10.1111/j.1365-2958.1994.tb00361.x;
RA   Lee S.J., Xie A., Jiang W., Etchegaray J.-P., Jones P.G., Inouye M.;
RT   "Family of the major cold-shock protein, CspA (CS7.4), of Escherichia coli,
RT   whose members show a high sequence similarity with the eukaryotic Y-box
RT   binding proteins.";
RL   Mol. Microbiol. 11:833-839(1994).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=K12;
RX   PubMed=7984109; DOI=10.1111/j.1365-2958.1994.tb00424.x;
RA   Yamanaka K., Mitani T., Ogura T., Niki H., Hiraga S.;
RT   "Cloning, sequencing, and characterization of multicopy suppressors of a
RT   mukB mutation in Escherichia coli.";
RL   Mol. Microbiol. 13:301-312(1994).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=9097040; DOI=10.1093/dnares/3.6.379;
RA   Itoh T., Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K.,
RA   Kasai H., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T.,
RA   Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S., Nakamura Y.,
RA   Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y.,
RA   Sivasundaram S., Tagami H., Takeda J., Takemoto K., Wada C., Yamamoto Y.,
RA   Horiuchi T.;
RT   "A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to
RT   the 40.1-50.0 min region on the linkage map.";
RL   DNA Res. 3:379-392(1996).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA   Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1462(1997).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT   and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
RN   [6]
RP   PROTEIN SEQUENCE OF 2-13.
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RA   Pasquali C., Sanchez J.-C., Ravier F., Golaz O., Hughes G.J., Frutiger S.,
RA   Paquet N., Wilkins M., Appel R.D., Bairoch A., Hochstrasser D.F.;
RL   Submitted (SEP-1994) to UniProtKB.
RN   [7]
RP   PROTEIN SEQUENCE OF 2-13.
RC   STRAIN=K12 / EMG2;
RX   PubMed=9298646; DOI=10.1002/elps.1150180807;
RA   Link A.J., Robison K., Church G.M.;
RT   "Comparing the predicted and observed properties of proteins encoded in the
RT   genome of Escherichia coli K-12.";
RL   Electrophoresis 18:1259-1313(1997).
RN   [8]
RP   PROTEIN SEQUENCE OF 2-11.
RC   STRAIN=K12;
RX   PubMed=9868784; DOI=10.1111/j.1574-6968.1998.tb13343.x;
RA   Wasinger V.C., Humphery-Smith I.;
RT   "Small genes/gene-products in Escherichia coli K-12.";
RL   FEMS Microbiol. Lett. 169:375-382(1998).
RN   [9]
RP   MASS SPECTROMETRY.
RC   STRAIN=ATCC 15597;
RX   PubMed=12071744; DOI=10.1021/ja025966k;
RA   Reid G.E., Shang H., Hogan J.M., Lee G.U., McLuckey S.A.;
RT   "Gas-phase concentration, purification, and identification of whole
RT   proteins from complex mixtures.";
RL   J. Am. Chem. Soc. 124:7353-7362(2002).
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- MASS SPECTROMETRY: Mass=7273; Method=Electrospray;
CC       Evidence={ECO:0000269|PubMed:12071744};
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DR   EMBL; L28430; AAA23619.1; -; Genomic_DNA.
DR   EMBL; D28496; BAA05854.1; -; Genomic_DNA.
DR   EMBL; U00096; AAC74893.1; -; Genomic_DNA.
DR   EMBL; AP009048; BAA15634.1; -; Genomic_DNA.
DR   PIR; S43618; S43618.
DR   RefSeq; NP_416337.1; NC_000913.3.
DR   RefSeq; WP_001062678.1; NZ_STEB01000009.1.
DR   AlphaFoldDB; P0A9Y6; -.
DR   SMR; P0A9Y6; -.
DR   BioGRID; 4263536; 354.
DR   BioGRID; 850696; 1.
DR   DIP; DIP-36183N; -.
DR   IntAct; P0A9Y6; 77.
DR   STRING; 511145.b1823; -.
DR   SWISS-2DPAGE; P0A9Y6; -.
DR   jPOST; P0A9Y6; -.
DR   PaxDb; P0A9Y6; -.
DR   PRIDE; P0A9Y6; -.
DR   EnsemblBacteria; AAC74893; AAC74893; b1823.
DR   EnsemblBacteria; BAA15634; BAA15634; BAA15634.
DR   GeneID; 8911702; -.
DR   GeneID; 946339; -.
DR   KEGG; ecj:JW1812; -.
DR   KEGG; eco:b1823; -.
DR   PATRIC; fig|1411691.4.peg.428; -.
DR   EchoBASE; EB2120; -.
DR   eggNOG; COG1278; Bacteria.
DR   HOGENOM; CLU_117621_2_1_6; -.
DR   InParanoid; P0A9Y6; -.
DR   OMA; FEIQDGH; -.
DR   PhylomeDB; P0A9Y6; -.
DR   BioCyc; EcoCyc:EG12204-MON; -.
DR   PRO; PR:P0A9Y6; -.
DR   Proteomes; UP000000318; Chromosome.
DR   Proteomes; UP000000625; Chromosome.
DR   GO; GO:0005829; C:cytosol; IDA:EcoCyc.
DR   GO; GO:0016020; C:membrane; HDA:UniProtKB.
DR   GO; GO:0051087; F:chaperone binding; IDA:CACAO.
DR   GO; GO:0003676; F:nucleic acid binding; IBA:GO_Central.
DR   GO; GO:0003723; F:RNA binding; IDA:EcoliWiki.
DR   GO; GO:0003697; F:single-stranded DNA binding; IDA:EcoliWiki.
DR   GO; GO:0001072; F:transcription antitermination factor activity, RNA binding; IDA:EcoliWiki.
DR   GO; GO:0060567; P:negative regulation of DNA-templated transcription, termination; IDA:EcoliWiki.
DR   GO; GO:0010468; P:regulation of gene expression; IBA:GO_Central.
DR   CDD; cd04458; CSP_CDS; 1.
DR   Gene3D; 2.40.50.140; -; 1.
DR   InterPro; IPR012156; Cold_shock_CspA.
DR   InterPro; IPR011129; CSD.
DR   InterPro; IPR019844; CSD_1.
DR   InterPro; IPR002059; CSP_DNA-bd.
DR   InterPro; IPR012340; NA-bd_OB-fold.
DR   Pfam; PF00313; CSD; 1.
DR   PIRSF; PIRSF002599; Cold_shock_A; 1.
DR   PRINTS; PR00050; COLDSHOCK.
DR   SMART; SM00357; CSP; 1.
DR   SUPFAM; SSF50249; SSF50249; 1.
DR   PROSITE; PS00352; CSD_1; 1.
DR   PROSITE; PS51857; CSD_2; 1.
PE   1: Evidence at protein level;
KW   Activator; Cytoplasm; Direct protein sequencing; DNA-binding;
KW   Reference proteome; Transcription; Transcription regulation.
FT   INIT_MET        1
FT                   /note="Removed"
FT                   /evidence="ECO:0000269|PubMed:9298646,
FT                   ECO:0000269|PubMed:9868784, ECO:0000269|Ref.6"
FT   CHAIN           2..69
FT                   /note="Cold shock-like protein CspC"
FT                   /id="PRO_0000100241"
FT   DOMAIN          6..66
FT                   /note="CSD"
FT   CONFLICT        7
FT                   /note="Q -> T (in Ref. 8; AA sequence)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   69 AA;  7402 MW;  2A644A4C96F4B425 CRC64;
     MAKIKGQVKW FNESKGFGFI TPADGSKDVF VHFSAIQGNG FKTLAEGQNV EFEIQDGQKG
     PAAVNVTAI