ID   CSPD_ECO57              Reviewed;          74 AA.
AC   P0A970; P24245;
DT   19-JUL-2005, integrated into UniProtKB/Swiss-Prot.
DT   19-JUL-2005, sequence version 1.
DT   03-AUG-2022, entry version 94.
DE   RecName: Full=Cold shock-like protein CspD;
DE            Short=CSP-D;
GN   Name=cspD; OrderedLocusNames=Z1117, ECs0966;
OS   Escherichia coli O157:H7.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83334;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=O157:H7 / EDL933 / ATCC 700927 / EHEC;
RX   PubMed=11206551; DOI=10.1038/35054089;
RA   Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J.,
RA   Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G.,
RA   Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J.,
RA   Davis N.W., Lim A., Dimalanta E.T., Potamousis K., Apodaca J.,
RA   Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A.,
RA   Blattner F.R.;
RT   "Genome sequence of enterohaemorrhagic Escherichia coli O157:H7.";
RL   Nature 409:529-533(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=O157:H7 / Sakai / RIMD 0509952 / EHEC;
RX   PubMed=11258796; DOI=10.1093/dnares/8.1.11;
RA   Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K.,
RA   Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T.,
RA   Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T., Kuhara S.,
RA   Shiba T., Hattori M., Shinagawa H.;
RT   "Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and
RT   genomic comparison with a laboratory strain K-12.";
RL   DNA Res. 8:11-22(2001).
CC   -!- FUNCTION: Inhibits DNA replication at both initiation and elongation
CC       steps, most probably by binding to the opened, single-stranded regions
CC       at replication forks. Plays a regulatory role in chromosomal
CC       replication in nutrient-depleted cells (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- MISCELLANEOUS: Binds single-stranded DNA and RNA, but not double-
CC       stranded DNA, through hydrophobic interaction without sequence
CC       specificity, resulting in a packed structure. {ECO:0000250}.
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DR   EMBL; AE005174; AAG55262.1; -; Genomic_DNA.
DR   EMBL; BA000007; BAB34389.1; -; Genomic_DNA.
DR   PIR; B85600; B85600.
DR   PIR; F90749; F90749.
DR   RefSeq; NP_308993.1; NC_002695.1.
DR   RefSeq; WP_000410785.1; NZ_SWKA01000005.1.
DR   AlphaFoldDB; P0A970; -.
DR   SMR; P0A970; -.
DR   STRING; 155864.EDL933_1014; -.
DR   EnsemblBacteria; AAG55262; AAG55262; Z1117.
DR   EnsemblBacteria; BAB34389; BAB34389; ECs_0966.
DR   GeneID; 67414639; -.
DR   GeneID; 917710; -.
DR   KEGG; ece:Z1117; -.
DR   KEGG; ecs:ECs_0966; -.
DR   PATRIC; fig|386585.9.peg.1082; -.
DR   eggNOG; COG1278; Bacteria.
DR   HOGENOM; CLU_117621_0_2_6; -.
DR   OMA; HYSTIKM; -.
DR   Proteomes; UP000000558; Chromosome.
DR   Proteomes; UP000002519; Chromosome.
DR   GO; GO:0005829; C:cytosol; IEA:UniProt.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0008156; P:negative regulation of DNA replication; IEA:UniProtKB-KW.
DR   GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:InterPro.
DR   CDD; cd04458; CSP_CDS; 1.
DR   Gene3D; 2.40.50.140; -; 1.
DR   InterPro; IPR012156; Cold_shock_CspA.
DR   InterPro; IPR011129; CSD.
DR   InterPro; IPR019844; CSD_1.
DR   InterPro; IPR002059; CSP_DNA-bd.
DR   InterPro; IPR012751; CspD.
DR   InterPro; IPR012340; NA-bd_OB-fold.
DR   Pfam; PF00313; CSD; 1.
DR   PIRSF; PIRSF002599; Cold_shock_A; 1.
DR   PRINTS; PR00050; COLDSHOCK.
DR   SMART; SM00357; CSP; 1.
DR   SUPFAM; SSF50249; SSF50249; 1.
DR   TIGRFAMs; TIGR02381; cspD; 1.
DR   PROSITE; PS00352; CSD_1; 1.
DR   PROSITE; PS51857; CSD_2; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; DNA replication inhibitor; DNA-binding; Reference proteome;
KW   RNA-binding.
FT   CHAIN           1..74
FT                   /note="Cold shock-like protein CspD"
FT                   /id="PRO_0000100249"
FT   DOMAIN          4..64
FT                   /note="CSD"
SQ   SEQUENCE   74 AA;  7969 MW;  C74AB028135BC22C CRC64;
     MEKGTVKWFN NAKGFGFICP EGGGEDIFAH YSTIQMDGYR TLKAGQSVQF DVHQGPKGNH
     ASVIVPVEVE AAVA