ID   CSPD_SHIFL              Reviewed;          74 AA.
AC   P0A971; P24245;
DT   19-JUL-2005, integrated into UniProtKB/Swiss-Prot.
DT   19-JUL-2005, sequence version 1.
DT   03-AUG-2022, entry version 100.
DE   RecName: Full=Cold shock-like protein CspD;
DE            Short=CSP-D;
GN   Name=cspD; OrderedLocusNames=SF0840, S0880;
OS   Shigella flexneri.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Shigella.
OX   NCBI_TaxID=623;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=301 / Serotype 2a;
RX   PubMed=12384590; DOI=10.1093/nar/gkf566;
RA   Jin Q., Yuan Z., Xu J., Wang Y., Shen Y., Lu W., Wang J., Liu H., Yang J.,
RA   Yang F., Zhang X., Zhang J., Yang G., Wu H., Qu D., Dong J., Sun L.,
RA   Xue Y., Zhao A., Gao Y., Zhu J., Kan B., Ding K., Chen S., Cheng H.,
RA   Yao Z., He B., Chen R., Ma D., Qiang B., Wen Y., Hou Y., Yu J.;
RT   "Genome sequence of Shigella flexneri 2a: insights into pathogenicity
RT   through comparison with genomes of Escherichia coli K12 and O157.";
RL   Nucleic Acids Res. 30:4432-4441(2002).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700930 / 2457T / Serotype 2a;
RX   PubMed=12704152; DOI=10.1128/iai.71.5.2775-2786.2003;
RA   Wei J., Goldberg M.B., Burland V., Venkatesan M.M., Deng W., Fournier G.,
RA   Mayhew G.F., Plunkett G. III, Rose D.J., Darling A., Mau B., Perna N.T.,
RA   Payne S.M., Runyen-Janecky L.J., Zhou S., Schwartz D.C., Blattner F.R.;
RT   "Complete genome sequence and comparative genomics of Shigella flexneri
RT   serotype 2a strain 2457T.";
RL   Infect. Immun. 71:2775-2786(2003).
CC   -!- FUNCTION: Inhibits DNA replication at both initiation and elongation
CC       steps, most probably by binding to the opened, single-stranded regions
CC       at replication forks. Plays a regulatory role in chromosomal
CC       replication in nutrient-depleted cells (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- MISCELLANEOUS: Binds single-stranded DNA and RNA, but not double-
CC       stranded DNA, through hydrophobic interaction without sequence
CC       specificity, resulting in a packed structure. {ECO:0000250}.
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DR   EMBL; AE005674; AAN42473.1; -; Genomic_DNA.
DR   EMBL; AE014073; AAP16345.1; -; Genomic_DNA.
DR   RefSeq; NP_706766.1; NC_004337.2.
DR   RefSeq; WP_000410785.1; NZ_WPGW01000037.1.
DR   AlphaFoldDB; P0A971; -.
DR   SMR; P0A971; -.
DR   STRING; 198214.SF0840; -.
DR   EnsemblBacteria; AAN42473; AAN42473; SF0840.
DR   EnsemblBacteria; AAP16345; AAP16345; S0880.
DR   GeneID; 1027556; -.
DR   GeneID; 67414639; -.
DR   KEGG; sfl:SF0840; -.
DR   KEGG; sfx:S0880; -.
DR   PATRIC; fig|198214.7.peg.969; -.
DR   HOGENOM; CLU_117621_0_2_6; -.
DR   OrthoDB; 2034748at2; -.
DR   Proteomes; UP000001006; Chromosome.
DR   Proteomes; UP000002673; Chromosome.
DR   GO; GO:0005829; C:cytosol; IEA:UniProt.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0008156; P:negative regulation of DNA replication; IEA:UniProtKB-KW.
DR   GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:InterPro.
DR   CDD; cd04458; CSP_CDS; 1.
DR   Gene3D; 2.40.50.140; -; 1.
DR   InterPro; IPR012156; Cold_shock_CspA.
DR   InterPro; IPR011129; CSD.
DR   InterPro; IPR019844; CSD_1.
DR   InterPro; IPR002059; CSP_DNA-bd.
DR   InterPro; IPR012751; CspD.
DR   InterPro; IPR012340; NA-bd_OB-fold.
DR   Pfam; PF00313; CSD; 1.
DR   PIRSF; PIRSF002599; Cold_shock_A; 1.
DR   PRINTS; PR00050; COLDSHOCK.
DR   SMART; SM00357; CSP; 1.
DR   SUPFAM; SSF50249; SSF50249; 1.
DR   TIGRFAMs; TIGR02381; cspD; 1.
DR   PROSITE; PS00352; CSD_1; 1.
DR   PROSITE; PS51857; CSD_2; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; DNA replication inhibitor; DNA-binding; Reference proteome;
KW   RNA-binding.
FT   CHAIN           1..74
FT                   /note="Cold shock-like protein CspD"
FT                   /id="PRO_0000100251"
FT   DOMAIN          4..64
FT                   /note="CSD"
SQ   SEQUENCE   74 AA;  7969 MW;  C74AB028135BC22C CRC64;
     MEKGTVKWFN NAKGFGFICP EGGGEDIFAH YSTIQMDGYR TLKAGQSVQF DVHQGPKGNH
     ASVIVPVEVE AAVA