ID   CSPE_ECOLW              Reviewed;          69 AA.
AC   E0J1Q3; H9XYU0;
DT   31-MAY-2011, integrated into UniProtKB/Swiss-Prot.
DT   02-NOV-2010, sequence version 1.
DT   03-AUG-2022, entry version 54.
DE   RecName: Full=Cold shock-like protein CspE;
DE            Short=CSP-E;
GN   Name=cspE; OrderedLocusNames=ECW_m0678, WFL_03370;
GN   ORFNames=EschWDRAFT_2558;
OS   Escherichia coli (strain ATCC 9637 / CCM 2024 / DSM 1116 / LMG 11080 / NBRC
OS   13500 / NCIMB 8666 / NRRL B-766 / W).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=566546;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 9637 / CCM 2024 / DSM 1116 / LMG 11080 / NBRC 13500 / NCIMB
RC   8666 / NRRL B-766 / W;
RG   US DOE Joint Genome Institute (JGI-PGF);
RA   Lucas S., Copeland A., Lapidus A., Cheng J.-F., Bruce D., Goodwin L.,
RA   Pitluck S., Land M.L., Hauser L., Chang Y.-J., Jeffries C., Tremaine M.,
RA   Landick R., Keating D., Woyke T.J.;
RT   "The draft genome of Escherichia coli W.";
RL   Submitted (JUL-2010) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 9637 / CCM 2024 / DSM 1116 / LMG 11080 / NBRC 13500 / NCIMB
RC   8666 / NRRL B-766 / W;
RX   PubMed=21208457; DOI=10.1186/1471-2164-12-9;
RA   Archer C.T., Kim J.F., Jeong H., Park J.H., Vickers C.E., Lee S.Y.,
RA   Nielsen L.K.;
RT   "The genome sequence of E. coli W (ATCC 9637): comparative genome analysis
RT   and an improved genome-scale reconstruction of E. coli.";
RL   BMC Genomics 12:9-9(2011).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 9637 / CCM 2024 / DSM 1116 / LMG 11080 / NBRC 13500 / NCIMB
RC   8666 / NRRL B-766 / W;
RX   PubMed=22075923; DOI=10.1007/s10295-011-1052-2;
RA   Turner P.C., Yomano L.P., Jarboe L.R., York S.W., Baggett C.L.,
RA   Moritz B.E., Zentz E.B., Shanmugam K.T., Ingram L.O.;
RT   "Optical mapping and sequencing of the Escherichia coli KO11 genome reveal
RT   extensive chromosomal rearrangements, and multiple tandem copies of the
RT   Zymomonas mobilis pdc and adhB genes.";
RL   J. Ind. Microbiol. Biotechnol. 39:629-639(2012).
RN   [4]
RP   MASS SPECTROMETRY.
RC   STRAIN=ATCC 9637 / CCM 2024 / DSM 1116 / LMG 11080 / NBRC 13500 / NCIMB
RC   8666 / NRRL B-766 / W;
RX   PubMed=9921691;
RX   DOI=10.1002/(sici)1097-0231(19990115)13:1<73::aid-rcm454>3.0.co;2-n;
RA   Dai Y., Li L., Roser D.C., Long S.R.;
RT   "Detection and identification of low-mass peptides and proteins from
RT   solvent suspensions of Escherichia coli by high performance liquid
RT   chromatography fractionation and matrix-assisted laser
RT   desorption/ionization mass spectrometry.";
RL   Rapid Commun. Mass Spectrom. 13:73-78(1999).
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- MASS SPECTROMETRY: Mass=7333.8; Method=MALDI;
CC       Evidence={ECO:0000269|PubMed:9921691};
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DR   EMBL; AEDF01000012; EFN37867.1; -; Genomic_DNA.
DR   EMBL; CP002185; ADT74206.1; -; Genomic_DNA.
DR   EMBL; CP002967; AFH10342.1; -; Genomic_DNA.
DR   RefSeq; WP_000034825.1; NZ_WBMH01000008.1.
DR   AlphaFoldDB; E0J1Q3; -.
DR   SMR; E0J1Q3; -.
DR   EnsemblBacteria; ADT74206; ADT74206; ECW_m0678.
DR   GeneID; 64171175; -.
DR   GeneID; 67516440; -.
DR   KEGG; ell:WFL_03370; -.
DR   KEGG; elw:ECW_m0678; -.
DR   PATRIC; fig|566546.30.peg.697; -.
DR   HOGENOM; CLU_117621_2_1_6; -.
DR   OMA; IWQKERG; -.
DR   Proteomes; UP000008525; Chromosome.
DR   GO; GO:0005829; C:cytosol; IEA:UniProt.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   CDD; cd04458; CSP_CDS; 1.
DR   Gene3D; 2.40.50.140; -; 1.
DR   InterPro; IPR012156; Cold_shock_CspA.
DR   InterPro; IPR011129; CSD.
DR   InterPro; IPR019844; CSD_1.
DR   InterPro; IPR002059; CSP_DNA-bd.
DR   InterPro; IPR012340; NA-bd_OB-fold.
DR   Pfam; PF00313; CSD; 1.
DR   PIRSF; PIRSF002599; Cold_shock_A; 1.
DR   PRINTS; PR00050; COLDSHOCK.
DR   SMART; SM00357; CSP; 1.
DR   SUPFAM; SSF50249; SSF50249; 1.
DR   PROSITE; PS00352; CSD_1; 1.
DR   PROSITE; PS51857; CSD_2; 1.
PE   1: Evidence at protein level;
KW   Activator; Cytoplasm; DNA-binding; Transcription; Transcription regulation.
FT   INIT_MET        1
FT                   /note="Removed"
FT                   /evidence="ECO:0000305"
FT   CHAIN           2..69
FT                   /note="Cold shock-like protein CspE"
FT                   /id="PRO_0000408739"
FT   DOMAIN          3..68
FT                   /note="CSD"
SQ   SEQUENCE   69 AA;  7463 MW;  1C33C920AA82405C CRC64;
     MSKIKGNVKW FNESKGFGFI TPEDGSKDVF VHFSAIQTNG FKTLAEGQRV EFEITNGAKG
     PSAANVIAL