ID   CSPF_ECOLI              Reviewed;          70 AA.
AC   P0A976; P39819;
DT   19-JUL-2005, integrated into UniProtKB/Swiss-Prot.
DT   19-JUL-2005, sequence version 1.
DT   03-AUG-2022, entry version 104.
DE   RecName: Full=Cold shock-like protein CspF;
DE            Short=CSP-F;
GN   Name=cspF; OrderedLocusNames=b1558, JW1550;
OS   Escherichia coli (strain K12).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=TAP90 / ATCC 47037;
RX   PubMed=8022261; DOI=10.1111/j.1365-2958.1994.tb00361.x;
RA   Lee S.J., Xie A., Jiang W., Etchegaray J.-P., Jones P.G., Inouye M.;
RT   "Family of the major cold-shock protein, CspA (CS7.4), of Escherichia coli,
RT   whose members show a high sequence similarity with the eukaryotic Y-box
RT   binding proteins.";
RL   Mol. Microbiol. 11:833-839(1994).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=9439003; DOI=10.1038/sj.jim.2900463;
RA   Francis K.P., Stewart G.S.A.B.;
RT   "Detection and speciation of bacteria through PCR using universal major
RT   cold-shock protein primer oligomers.";
RL   J. Ind. Microbiol. Biotechnol. 19:286-293(1997).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=9097039; DOI=10.1093/dnares/3.6.363;
RA   Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Itoh T.,
RA   Kasai H., Kashimoto K., Kimura S., Kitakawa M., Kitagawa M., Makino K.,
RA   Miki T., Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S.,
RA   Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G.,
RA   Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Takeuchi Y.,
RA   Wada C., Yamamoto Y., Horiuchi T.;
RT   "A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to
RT   the 28.0-40.1 min region on the linkage map.";
RL   DNA Res. 3:363-377(1996).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA   Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1462(1997).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT   and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
RN   [6]
RP   IDENTIFICATION.
RA   Rudd K.E.;
RL   Unpublished observations (NOV-1994).
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
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DR   EMBL; L28429; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; AF003590; AAB61740.1; -; Genomic_DNA.
DR   EMBL; U00096; AAC74631.1; -; Genomic_DNA.
DR   EMBL; AP009048; BAA15257.1; -; Genomic_DNA.
DR   PIR; A64911; A64911.
DR   RefSeq; NP_416076.1; NC_000913.3.
DR   RefSeq; WP_000087756.1; NZ_SSUV01000001.1.
DR   AlphaFoldDB; P0A976; -.
DR   SMR; P0A976; -.
DR   BioGRID; 4260241; 9.
DR   STRING; 511145.b1558; -.
DR   PaxDb; P0A976; -.
DR   PRIDE; P0A976; -.
DR   EnsemblBacteria; AAC74631; AAC74631; b1558.
DR   EnsemblBacteria; BAA15257; BAA15257; BAA15257.
DR   GeneID; 67415736; -.
DR   GeneID; 946090; -.
DR   KEGG; ecj:JW1550; -.
DR   KEGG; eco:b1558; -.
DR   PATRIC; fig|1411691.4.peg.705; -.
DR   EchoBASE; EB2121; -.
DR   eggNOG; COG1278; Bacteria.
DR   HOGENOM; CLU_117621_2_1_6; -.
DR   InParanoid; P0A976; -.
DR   OMA; FQKMTGI; -.
DR   PhylomeDB; P0A976; -.
DR   BioCyc; EcoCyc:EG12205-MON; -.
DR   PRO; PR:P0A976; -.
DR   Proteomes; UP000000318; Chromosome.
DR   Proteomes; UP000000625; Chromosome.
DR   GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003676; F:nucleic acid binding; IBA:GO_Central.
DR   GO; GO:0003723; F:RNA binding; IBA:GO_Central.
DR   GO; GO:0001072; F:transcription antitermination factor activity, RNA binding; IBA:GO_Central.
DR   GO; GO:0060567; P:negative regulation of DNA-templated transcription, termination; IBA:GO_Central.
DR   GO; GO:0010468; P:regulation of gene expression; IBA:GO_Central.
DR   CDD; cd04458; CSP_CDS; 1.
DR   Gene3D; 2.40.50.140; -; 1.
DR   InterPro; IPR012156; Cold_shock_CspA.
DR   InterPro; IPR011129; CSD.
DR   InterPro; IPR019844; CSD_1.
DR   InterPro; IPR002059; CSP_DNA-bd.
DR   InterPro; IPR012340; NA-bd_OB-fold.
DR   Pfam; PF00313; CSD; 1.
DR   PIRSF; PIRSF002599; Cold_shock_A; 1.
DR   SMART; SM00357; CSP; 1.
DR   SUPFAM; SSF50249; SSF50249; 1.
DR   PROSITE; PS00352; CSD_1; 1.
DR   PROSITE; PS51857; CSD_2; 1.
PE   3: Inferred from homology;
KW   Activator; Cytoplasm; DNA-binding; Reference proteome; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..70
FT                   /note="Cold shock-like protein CspF"
FT                   /id="PRO_0000100260"
FT   DOMAIN          7..67
FT                   /note="CSD"
SQ   SEQUENCE   70 AA;  7563 MW;  1C50C17BCC3D0E5C CRC64;
     MSRKMTGIVK TFDGKSGKGL ITPSDGRIDV QLHVSALNLR DAEEITTGLR VEFCRINGLR
     GPSAANVYLS