ID   CSPL6_PICSI             Reviewed;         196 AA.
AC   A9P1V1;
DT   14-APR-2009, integrated into UniProtKB/Swiss-Prot.
DT   05-FEB-2008, sequence version 1.
DT   03-AUG-2022, entry version 31.
DE   RecName: Full=CASP-like protein 2A2;
DE            Short=PsCASPL2A2;
OS   Picea sitchensis (Sitka spruce) (Pinus sitchensis).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Pinopsida; Pinidae; Conifers I; Pinales; Pinaceae; Picea.
OX   NCBI_TaxID=3332;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. FB3-425;
RA   Ralph S.G., Kirkpatrick R., Chun H.J.E., Palmquist D., Wynhoven B.,
RA   Kolosova N., Cooper N., Oddy C., Jancsik S., Ritland C.E., Douglas C.J.,
RA   Butterfield Y.S.N., Liu J., Stott J., Yang G., Barber S., Holt R.A.,
RA   Siddiqui A., Jones S.J.M., Marra M.A., Ritland K., Bohlmann J.;
RT   "The spruce transcriptome: analysis of ca. 6,500 sequence-verified full-
RT   length cDNAs.";
RL   Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   GENE FAMILY, AND NOMENCLATURE.
RX   PubMed=24920445; DOI=10.1104/pp.114.239137;
RA   Roppolo D., Boeckmann B., Pfister A., Boutet E., Rubio M.C.,
RA   Denervaud-Tendon V., Vermeer J.E., Gheyselinck J., Xenarios I., Geldner N.;
RT   "Functional and evolutionary analysis of the CASPARIAN STRIP MEMBRANE
RT   DOMAIN PROTEIN family.";
RL   Plant Physiol. 165:1709-1722(2014).
CC   -!- SUBUNIT: Homodimer and heterodimers. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250}; Multi-pass membrane
CC       protein {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the Casparian strip membrane proteins (CASP)
CC       family. {ECO:0000305}.
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DR   EMBL; EF087625; ABK26862.1; -; mRNA.
DR   AlphaFoldDB; A9P1V1; -.
DR   SMR; A9P1V1; -.
DR   OMA; NGVCAGY; -.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   InterPro; IPR006459; CASP/CASPL.
DR   InterPro; IPR006702; CASP_dom.
DR   Pfam; PF04535; DUF588; 1.
DR   TIGRFAMs; TIGR01569; A_tha_TIGR01569; 1.
PE   2: Evidence at transcript level;
KW   Cell membrane; Membrane; Transmembrane; Transmembrane helix.
FT   CHAIN           1..196
FT                   /note="CASP-like protein 2A2"
FT                   /id="PRO_0000370319"
FT   TOPO_DOM        1..26
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        27..47
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        48..68
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        69..89
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        90..98
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        99..119
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        120..148
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        149..169
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        170..196
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   196 AA;  21493 MW;  DD29ABA3F3932952 CRC64;
     MAQGKESVSV VEMEGSGNGP AVEMRHFETL FRLLPVGLCI SALVLMLKSE QSDQYMQLDY
     SNVDAFRCLA YANGICAGYS LISAFDSMVP VSHHISRSWI LFLLDQGITY LMLAGGAVAT
     QVLYVAYKGD EKATWEQICG SYGRFCNRAG ASVIISFFAL VCFLLLSLLS AYRLFSKYDP
     PIHGGAKLED QTTAQI