ID   CSPLY_ARATH             Reviewed;         297 AA.
AC   Q501G6; Q9FM13;
DT   23-OCT-2007, integrated into UniProtKB/Swiss-Prot.
DT   07-JUN-2005, sequence version 1.
DT   25-MAY-2022, entry version 84.
DE   RecName: Full=CASP-like protein 4A2;
DE            Short=AtCASPL4A2;
GN   OrderedLocusNames=At5g62820; ORFNames=MQB2.14;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=9628582; DOI=10.1093/dnares/5.1.41;
RA   Sato S., Kaneko T., Kotani H., Nakamura Y., Asamizu E., Miyajima N.,
RA   Tabata S.;
RT   "Structural analysis of Arabidopsis thaliana chromosome 5. IV. Sequence
RT   features of the regions of 1,456,315 bp covered by nineteen physically
RT   assigned P1 and TAC clones.";
RL   DNA Res. 5:41-54(1998).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RA   Kim C.J., Chen H., Cheuk R.F., Shinn P., Ecker J.R.;
RT   "Arabidopsis ORF clones.";
RL   Submitted (JUN-2005) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   GENE FAMILY, AND NOMENCLATURE.
RX   PubMed=24920445; DOI=10.1104/pp.114.239137;
RA   Roppolo D., Boeckmann B., Pfister A., Boutet E., Rubio M.C.,
RA   Denervaud-Tendon V., Vermeer J.E., Gheyselinck J., Xenarios I., Geldner N.;
RT   "Functional and evolutionary analysis of the CASPARIAN STRIP MEMBRANE
RT   DOMAIN PROTEIN family.";
RL   Plant Physiol. 165:1709-1722(2014).
CC   -!- SUBUNIT: Homodimer and heterodimers. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250}; Multi-pass membrane
CC       protein {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the Casparian strip membrane proteins (CASP)
CC       family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=BAB10851.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR   EMBL; AB009053; BAB10851.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; CP002688; AED97661.1; -; Genomic_DNA.
DR   EMBL; BT022001; AAY25413.1; -; mRNA.
DR   EMBL; BT023479; AAY57318.1; -; mRNA.
DR   RefSeq; NP_201088.1; NM_125677.4.
DR   AlphaFoldDB; Q501G6; -.
DR   STRING; 3702.AT5G62820.1; -.
DR   iPTMnet; Q501G6; -.
DR   PaxDb; Q501G6; -.
DR   PRIDE; Q501G6; -.
DR   ProteomicsDB; 222691; -.
DR   EnsemblPlants; AT5G62820.1; AT5G62820.1; AT5G62820.
DR   GeneID; 836403; -.
DR   Gramene; AT5G62820.1; AT5G62820.1; AT5G62820.
DR   KEGG; ath:AT5G62820; -.
DR   Araport; AT5G62820; -.
DR   TAIR; locus:2170703; AT5G62820.
DR   eggNOG; ENOG502QW75; Eukaryota.
DR   HOGENOM; CLU_048961_2_0_1; -.
DR   InParanoid; Q501G6; -.
DR   OMA; PFKIENP; -.
DR   OrthoDB; 1502083at2759; -.
DR   PhylomeDB; Q501G6; -.
DR   PRO; PR:Q501G6; -.
DR   Proteomes; UP000006548; Chromosome 5.
DR   ExpressionAtlas; Q501G6; baseline and differential.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IBA:GO_Central.
DR   InterPro; IPR006702; CASP_dom.
DR   Pfam; PF04535; DUF588; 1.
PE   2: Evidence at transcript level;
KW   Cell membrane; Membrane; Reference proteome; Transmembrane;
KW   Transmembrane helix.
FT   CHAIN           1..297
FT                   /note="CASP-like protein 4A2"
FT                   /id="PRO_0000308689"
FT   TOPO_DOM        1..149
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        150..170
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        171..191
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        192..212
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        213..225
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        226..246
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        247..265
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        266..286
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        287..297
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   REGION          1..135
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1..24
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        68..85
FT                   /note="Pro residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        89..135
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   297 AA;  32612 MW;  9109D4AF07009400 CRC64;
     MKMKRTASSN SEAQSYNESP HSPLRFHSPL SDAGDLPESR YVSPEGSPFK IENPKSIVAG
     NKLTQFSPLP SPIPPPPPQF PPPRRQRNAR VPMNSSSDKS PSSMVVHNSW VREDGGQTTT
     RKAGAPINGE ESTRTTVNRA RGDDLVSLTA LGFRITEVIL CVISFSIMAA DKTQGWSGDS
     YDRYKEYRYC LAVNVIAFVY SAFEACDAAC YIAKESYMIN CGFHDLFVFS MDQLLAYLLM
     SASSCAATRV DDWVSNWGKD EFTQMATASI AVSFLAFGAF AVSALISSYR LFTHASS