ID   CSP_THEMA               Reviewed;          66 AA.
AC   O54310;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-1998, sequence version 1.
DT   25-MAY-2022, entry version 140.
DE   RecName: Full=Cold shock-like protein;
GN   Name=csp; OrderedLocusNames=TM_1683;
OS   Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826
OS   / MSB8).
OC   Bacteria; Thermotogae; Thermotogales; Thermotogaceae; Thermotoga.
OX   NCBI_TaxID=243274;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PARTIAL PROTEIN SEQUENCE.
RC   STRAIN=ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8;
RX   PubMed=10048332; DOI=10.1110/ps.8.2.394;
RA   Welker C., Bohm G., Schurig H., Jaenicke R.;
RT   "Cloning, overexpression, purification, and physicochemical
RT   characterization of a cold shock protein homolog from the hyperthermophilic
RT   bacterium Thermotoga maritima.";
RL   Protein Sci. 8:394-403(1999).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8;
RX   PubMed=10360571; DOI=10.1038/20601;
RA   Nelson K.E., Clayton R.A., Gill S.R., Gwinn M.L., Dodson R.J., Haft D.H.,
RA   Hickey E.K., Peterson J.D., Nelson W.C., Ketchum K.A., McDonald L.A.,
RA   Utterback T.R., Malek J.A., Linher K.D., Garrett M.M., Stewart A.M.,
RA   Cotton M.D., Pratt M.S., Phillips C.A., Richardson D.L., Heidelberg J.F.,
RA   Sutton G.G., Fleischmann R.D., Eisen J.A., White O., Salzberg S.L.,
RA   Smith H.O., Venter J.C., Fraser C.M.;
RT   "Evidence for lateral gene transfer between Archaea and Bacteria from
RT   genome sequence of Thermotoga maritima.";
RL   Nature 399:323-329(1999).
RN   [3]
RP   MUTAGENESIS.
RX   PubMed=10431826; DOI=10.1016/s0014-5793(99)00829-7;
RA   Frankenberg N., Welker C., Jaenicke R.;
RT   "Does the elimination of ion pairs affect the thermal stability of cold
RT   shock protein from the hyperthermophilic bacterium Thermotoga maritima?";
RL   FEBS Lett. 454:299-302(1999).
RN   [4]
RP   STRUCTURE BY NMR.
RX   PubMed=11322871; DOI=10.1046/j.1432-1327.2001.02127.x;
RA   Kremer W., Schuler B., Harrieder S., Geyer M., Gronwald W., Welker C.,
RA   Jaenicke R., Kalbitzer H.R.;
RT   "Solution NMR structure of the cold-shock protein from the
RT   hyperthermophilic bacterium Thermotoga maritima.";
RL   Eur. J. Biochem. 268:2527-2539(2001).
CC   -!- SUBUNIT: Monomer.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
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DR   EMBL; Y11219; CAA72105.1; -; mRNA.
DR   EMBL; AE000512; AAD36750.1; -; Genomic_DNA.
DR   PIR; E72222; E72222.
DR   RefSeq; NP_229483.1; NC_000853.1.
DR   RefSeq; WP_004082199.1; NZ_CP011107.1.
DR   PDB; 1G6P; NMR; -; A=1-66.
DR   PDBsum; 1G6P; -.
DR   AlphaFoldDB; O54310; -.
DR   BMRB; O54310; -.
DR   SASBDB; O54310; -.
DR   SMR; O54310; -.
DR   STRING; 243274.THEMA_05825; -.
DR   EnsemblBacteria; AAD36750; AAD36750; TM_1683.
DR   KEGG; tma:TM1683; -.
DR   eggNOG; COG1278; Bacteria.
DR   InParanoid; O54310; -.
DR   OMA; HYSTIKM; -.
DR   OrthoDB; 2034748at2; -.
DR   EvolutionaryTrace; O54310; -.
DR   Proteomes; UP000008183; Chromosome.
DR   GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003676; F:nucleic acid binding; IBA:GO_Central.
DR   GO; GO:0003723; F:RNA binding; IBA:GO_Central.
DR   GO; GO:0001072; F:transcription antitermination factor activity, RNA binding; IBA:GO_Central.
DR   GO; GO:0060567; P:negative regulation of DNA-templated transcription, termination; IBA:GO_Central.
DR   GO; GO:0010468; P:regulation of gene expression; IBA:GO_Central.
DR   CDD; cd04458; CSP_CDS; 1.
DR   Gene3D; 2.40.50.140; -; 1.
DR   InterPro; IPR012156; Cold_shock_CspA.
DR   InterPro; IPR011129; CSD.
DR   InterPro; IPR019844; CSD_1.
DR   InterPro; IPR002059; CSP_DNA-bd.
DR   InterPro; IPR012340; NA-bd_OB-fold.
DR   Pfam; PF00313; CSD; 1.
DR   PIRSF; PIRSF002599; Cold_shock_A; 1.
DR   PRINTS; PR00050; COLDSHOCK.
DR   SMART; SM00357; CSP; 1.
DR   SUPFAM; SSF50249; SSF50249; 1.
DR   PROSITE; PS00352; CSD_1; 1.
DR   PROSITE; PS51857; CSD_2; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Activator; Cytoplasm; Direct protein sequencing; DNA-binding;
KW   Reference proteome; Transcription; Transcription regulation.
FT   CHAIN           1..66
FT                   /note="Cold shock-like protein"
FT                   /id="PRO_0000100339"
FT   DOMAIN          3..62
FT                   /note="CSD"
FT   MUTAGEN         9
FT                   /note="D->N: Destabilization at high temperature."
FT                   /evidence="ECO:0000269|PubMed:10431826"
FT   MUTAGEN         61
FT                   /note="H->N: No effect on thermal stability."
FT                   /evidence="ECO:0000269|PubMed:10431826"
FT   STRAND          3..9
FT                   /evidence="ECO:0007829|PDB:1G6P"
FT   TURN            10..13
FT                   /evidence="ECO:0007829|PDB:1G6P"
FT   STRAND          14..19
FT                   /evidence="ECO:0007829|PDB:1G6P"
FT   STRAND          29..32
FT                   /evidence="ECO:0007829|PDB:1G6P"
FT   STRAND          42..51
FT                   /evidence="ECO:0007829|PDB:1G6P"
FT   STRAND          53..55
FT                   /evidence="ECO:0007829|PDB:1G6P"
FT   STRAND          58..65
FT                   /evidence="ECO:0007829|PDB:1G6P"
SQ   SEQUENCE   66 AA;  7473 MW;  851E3B79C6114E8E CRC64;
     MRGKVKWFDS KKGYGFITKD EGGDVFVHWS AIEMEGFKTL KEGQVVEFEI QEGKKGPQAA
     HVKVVE