CSQR_ECOLI
ID CSQR_ECOLI Reviewed; 261 AA.
AC P32144; Q2M8I1;
DT 01-OCT-1993, integrated into UniProtKB/Swiss-Prot.
DT 27-APR-2001, sequence version 2.
DT 03-AUG-2022, entry version 154.
DE RecName: Full=HTH-type transcriptional repressor CsqR {ECO:0000305};
DE AltName: Full=Regulator of sulfoquinovose catabolism {ECO:0000303|PubMed:30372406};
GN Name=csqR {ECO:0000303|PubMed:30372406}; Synonyms=yihW;
GN OrderedLocusNames=b3884, JW5567;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=8346018; DOI=10.1093/nar/21.15.3391;
RA Plunkett G. III, Burland V., Daniels D.L., Blattner F.R.;
RT "Analysis of the Escherichia coli genome. III. DNA sequence of the region
RT from 87.2 to 89.2 minutes.";
RL Nucleic Acids Res. 21:3391-3398(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [4]
RP PROBABLE FUNCTION.
RC STRAIN=K12;
RX PubMed=24463506; DOI=10.1038/nature12947;
RA Denger K., Weiss M., Felux A.K., Schneider A., Mayer C., Spiteller D.,
RA Huhn T., Cook A.M., Schleheck D.;
RT "Sulphoglycolysis in Escherichia coli K-12 closes a gap in the
RT biogeochemical sulphur cycle.";
RL Nature 507:114-117(2014).
RN [5]
RP FUNCTION, DNA-BINDING, ACTIVITY REGULATION, SUBUNIT, AND INDUCTION.
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=30372406; DOI=10.1099/mic.0.000740;
RA Shimada T., Yamamoto K., Nakano M., Watanabe H., Schleheck D., Ishihama A.;
RT "Regulatory role of CsqR (YihW) in transcription of the genes for
RT catabolism of the anionic sugar sulfoquinovose (SQ) in Escherichia coli K-
RT 12.";
RL Microbiology 165:78-89(2019).
CC -!- FUNCTION: Involved in the regulation of the sulfoquinovose operon
CC (PubMed:24463506, PubMed:30372406). Represses the expression of the
CC yihUTS operon and of the yihV and csqR genes. Binds DNA inside the
CC spacer between the bidirectional transcription units comprising the
CC yihUTS operon and the yihV gene, and upstream the csqR gene itself
CC (PubMed:30372406). {ECO:0000269|PubMed:30372406,
CC ECO:0000305|PubMed:24463506}.
CC -!- ACTIVITY REGULATION: Inactivated in the presence of the effectors
CC sulfoquinovose and sulfoquinovosyl glycerol, leading to the de-
CC repression of the target genes. {ECO:0000269|PubMed:30372406}.
CC -!- SUBUNIT: Monomer in the absence of DNA. Exhibits a high level of
CC cooperativity once it is bound to its target DNA.
CC {ECO:0000269|PubMed:30372406}.
CC -!- INDUCTION: Negatively autoregulated. {ECO:0000269|PubMed:30372406}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAB03017.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR EMBL; L19201; AAB03017.1; ALT_INIT; Genomic_DNA.
DR EMBL; U00096; AAD13446.2; -; Genomic_DNA.
DR EMBL; AP009048; BAE77425.1; -; Genomic_DNA.
DR RefSeq; NP_418320.2; NC_000913.3.
DR RefSeq; WP_000059678.1; NZ_STEB01000017.1.
DR AlphaFoldDB; P32144; -.
DR SMR; P32144; -.
DR BioGRID; 4260965; 3.
DR DIP; DIP-12504N; -.
DR STRING; 511145.b3884; -.
DR jPOST; P32144; -.
DR PaxDb; P32144; -.
DR PRIDE; P32144; -.
DR EnsemblBacteria; AAD13446; AAD13446; b3884.
DR EnsemblBacteria; BAE77425; BAE77425; BAE77425.
DR GeneID; 66672210; -.
DR GeneID; 948381; -.
DR KEGG; ecj:JW5567; -.
DR KEGG; eco:b3884; -.
DR PATRIC; fig|1411691.4.peg.2827; -.
DR EchoBASE; EB1795; -.
DR eggNOG; COG1349; Bacteria.
DR HOGENOM; CLU_060699_0_0_6; -.
DR InParanoid; P32144; -.
DR OMA; IRRDFDQ; -.
DR PhylomeDB; P32144; -.
DR BioCyc; EcoCyc:EG11849-MON; -.
DR PRO; PR:P32144; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0003677; F:DNA binding; IDA:EcoCyc.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR GO; GO:2000143; P:negative regulation of DNA-templated transcription, initiation; IEP:EcoCyc.
DR CDD; cd00090; HTH_ARSR; 1.
DR Gene3D; 1.10.10.10; -; 1.
DR InterPro; IPR011991; ArsR-like_HTH.
DR InterPro; IPR014036; DeoR_C.
DR InterPro; IPR001034; DeoR_HTH.
DR InterPro; IPR037171; NagB/RpiA_transferase-like.
DR InterPro; IPR018356; Tscrpt_reg_HTH_DeoR_CS.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR Pfam; PF00455; DeoRC; 1.
DR Pfam; PF08220; HTH_DeoR; 1.
DR PRINTS; PR00037; HTHLACR.
DR SMART; SM00420; HTH_DEOR; 1.
DR SUPFAM; SSF100950; SSF100950; 1.
DR SUPFAM; SSF46785; SSF46785; 1.
DR PROSITE; PS00894; HTH_DEOR_1; 1.
DR PROSITE; PS51000; HTH_DEOR_2; 1.
PE 1: Evidence at protein level;
KW DNA-binding; Reference proteome; Repressor; Transcription;
KW Transcription regulation.
FT CHAIN 1..261
FT /note="HTH-type transcriptional repressor CsqR"
FT /id="PRO_0000050276"
FT DOMAIN 8..63
FT /note="HTH deoR-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00349"
FT DNA_BIND 25..44
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00349"
SQ SEQUENCE 261 AA; 28507 MW; A529920D22C9DD19 CRC64;
MSLTELTGNP RHDQLLMLIA ERGYMNIDEL ANLLDVSTQT VRRDIRKLSE QGLITRHHGG
AGRASSVVNT AFEQREVSQT EEKKAIAEAV ADYIPDGSTI FITIGTTVEH VARALLNHNH
LRIITNSLRV AHILYHNPRF EVMVPGGTLR SHNSGIIGPS AASFVADFRA DYLVTSVGAI
ESDGALMEFD VNEANVVKTM MAHARNILLV ADHTKYHASA AVEIGNVAQV TALFTDELPP
AALKSRLQDS QIEIILPQED A
