ID   CSS2_YEAST              Reviewed;         232 AA.
AC   P43600; I2HB58;
DT   01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1995, sequence version 1.
DT   03-AUG-2022, entry version 97.
DE   RecName: Full=Secreted protein CSS2 {ECO:0000303|PubMed:26928762};
DE   AltName: Full=Condition specific secretion protein 2 {ECO:0000303|PubMed:26928762};
DE   Flags: Precursor;
GN   Name=CSS2 {ECO:0000303|PubMed:26928762}; OrderedLocusNames=YFR020W;
OS   Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=559292;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=7670463; DOI=10.1038/ng0795-261;
RA   Murakami Y., Naitou M., Hagiwara H., Shibata T., Ozawa M., Sasanuma S.,
RA   Sasanuma M., Tsuchiya Y., Soeda E., Yokoyama K., Yamazaki M., Tashiro H.,
RA   Eki T.;
RT   "Analysis of the nucleotide sequence of chromosome VI from Saccharomyces
RT   cerevisiae.";
RL   Nat. Genet. 10:261-268(1995).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=24374639; DOI=10.1534/g3.113.008995;
RA   Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA   Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA   Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT   "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL   G3 (Bethesda) 4:389-398(2014).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=17322287; DOI=10.1101/gr.6037607;
RA   Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA   Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA   Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA   Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA   LaBaer J.;
RT   "Approaching a complete repository of sequence-verified protein-encoding
RT   clones for Saccharomyces cerevisiae.";
RL   Genome Res. 17:536-543(2007).
RN   [4]
RP   GLYCOSYLATION [LARGE SCALE ANALYSIS].
RX   PubMed=19756047; DOI=10.1038/msb.2009.64;
RA   Kung L.A., Tao S.-C., Qian J., Smith M.G., Snyder M., Zhu H.;
RT   "Global analysis of the glycoproteome in Saccharomyces cerevisiae reveals
RT   new roles for protein glycosylation in eukaryotes.";
RL   Mol. Syst. Biol. 5:308-308(2009).
RN   [5]
RP   IDENTIFICATION.
RX   PubMed=22194413; DOI=10.1126/science.1215110;
RA   Brar G.A., Yassour M., Friedman N., Regev A., Ingolia N.T., Weissman J.S.;
RT   "High-resolution view of the yeast meiotic program revealed by ribosome
RT   profiling.";
RL   Science 335:552-557(2012).
RN   [6]
RP   SUBCELLULAR LOCATION.
RX   PubMed=26928762; DOI=10.1038/nmeth.3795;
RA   Yofe I., Weill U., Meurer M., Chuartzman S., Zalckvar E., Goldman O.,
RA   Ben-Dor S., Schuetze C., Wiedemann N., Knop M., Khmelinskii A.,
RA   Schuldiner M.;
RT   "One library to make them all: streamlining the creation of yeast libraries
RT   via a SWAp-Tag strategy.";
RL   Nat. Methods 13:371-378(2016).
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:26928762}.
CC   -!- PTM: N-glycosylated. {ECO:0000269|PubMed:19756047}.
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DR   EMBL; D50617; BAA09259.1; -; Genomic_DNA.
DR   EMBL; AY558471; AAS56797.1; -; Genomic_DNA.
DR   EMBL; BK006940; DAA35105.1; -; Genomic_DNA.
DR   PIR; S56275; S56275.
DR   RefSeq; NP_001257674.1; NM_001270745.1.
DR   AlphaFoldDB; P43600; -.
DR   BioGRID; 300363; 7.
DR   STRING; 4932.YFR020W; -.
DR   PaxDb; P43600; -.
DR   EnsemblFungi; YFR020W_mRNA; YFR020W; YFR020W.
DR   GeneID; 850576; -.
DR   KEGG; sce:YFR020W; -.
DR   SGD; S000001916; CSS2.
DR   VEuPathDB; FungiDB:YFR020W; -.
DR   HOGENOM; CLU_1192391_0_0_1; -.
DR   InParanoid; P43600; -.
DR   OMA; NCNSTIH; -.
DR   BioCyc; YEAST:G3O-30471-MON; -.
DR   PRO; PR:P43600; -.
DR   Proteomes; UP000002311; Chromosome VI.
DR   RNAct; P43600; protein.
DR   GO; GO:0005783; C:endoplasmic reticulum; HDA:SGD.
DR   GO; GO:0005576; C:extracellular region; HDA:SGD.
PE   1: Evidence at protein level;
KW   Glycoprotein; Reference proteome; Secreted; Signal.
FT   SIGNAL          1..17
FT                   /evidence="ECO:0000255"
FT   CHAIN           18..232
FT                   /note="Secreted protein CSS2"
FT                   /id="PRO_0000202689"
FT   CARBOHYD        25
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        26
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        53
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        77
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        163
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   232 AA;  25721 MW;  2DFCB3249511B7A0 CRC64;
     MWCYSHFLLI FVSFVTSFAH KLPANNSTTN GGTDGIAVPV IETTIDSGMY SENGTDLMTP
     EDLPDLLSDG IVLSFANTTE TGSDSDSTLI DSEDLRRCID MPDRSCSAQR GNLCSYSFWD
     IPFSFLNTVH DIFGMTNMGN CAVMAGDKGA FYYKYYPVEP NCNSTIHQKT IDDALQQATE
     QLNGDFNNMY FFHVNRGGLW QGDMMVGTRV FTWFAGAKWA EHKGSIEAGF TS