CSSS_BACSU
ID CSSS_BACSU Reviewed; 451 AA.
AC O32193; O32303;
DT 26-JUL-2002, integrated into UniProtKB/Swiss-Prot.
DT 28-JUL-2009, sequence version 2.
DT 03-AUG-2022, entry version 146.
DE RecName: Full=Sensor histidine kinase CssS;
DE EC=2.7.13.3;
GN Name=cssS; Synonyms=yvqB; OrderedLocusNames=BSU33020;
OS Bacillus subtilis (strain 168).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=224308;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=9353931; DOI=10.1099/00221287-143-10-3305;
RA Medina N., Vannier F., Roche B., Autret S., Levine A., Seror S.J.;
RT "Sequencing of regions downstream of addA (98 degrees) and citG (289
RT degrees) in Bacillus subtilis.";
RL Microbiology 143:3305-3308(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9639930; DOI=10.1099/00221287-144-6-1593;
RA Wipat A., Brignell C.S., Guy J.B., Rose M., Emmerson P.T., Harwood C.R.;
RT "The yvsA-yvqA (293 degrees - 289 degrees) region of the Bacillus subtilis
RT chromosome containing genes involved in metal ion uptake and a putative
RT sigma factor.";
RL Microbiology 144:1593-1600(1998).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
RN [4]
RP SEQUENCE REVISION TO 444.
RX PubMed=19383706; DOI=10.1099/mic.0.027839-0;
RA Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A.,
RA Vallenet D., Wang T., Moszer I., Medigue C., Danchin A.;
RT "From a consortium sequence to a unified sequence: the Bacillus subtilis
RT 168 reference genome a decade later.";
RL Microbiology 155:1758-1775(2009).
RN [5]
RP FUNCTION.
RX PubMed=11717295; DOI=10.1128/jb.183.24.7365-7370.2001;
RA Kobayashi K., Ogura M., Yamaguchi H., Yoshida K., Ogasawara N., Tanaka T.,
RA Fujita Y.;
RT "Comprehensive DNA microarray analysis of Bacillus subtilis two-component
RT regulatory systems.";
RL J. Bacteriol. 183:7365-7370(2001).
RN [6]
RP CHARACTERIZATION.
RC STRAIN=168;
RX PubMed=11555295; DOI=10.1046/j.1365-2958.2001.02576.x;
RA Hyyrylaeinen H.-L., Bolhuis A., Darmon E., Muukkonen L., Koski P.,
RA Vitikainen M., Sarvas M., Pragai Z., Bron S., van Dijl J.M., Kontinen V.P.;
RT "A novel two-component regulatory system in Bacillus subtilis for the
RT survival of severe secretion stress.";
RL Mol. Microbiol. 41:1159-1172(2001).
CC -!- FUNCTION: Member of the two-component regulatory system CssS/CssR
CC required to control the cellular response to secretion stress. Required
CC for the transcription of htrA. Could detect misfolded proteins at the
CC membrane-cell wall interface and then activate CssR by phosphorylation.
CC {ECO:0000269|PubMed:11717295}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + protein L-histidine = ADP + protein N-phospho-L-
CC histidine.; EC=2.7.13.3;
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAB07976.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; Z93941; CAB07976.1; ALT_INIT; Genomic_DNA.
DR EMBL; AJ223978; CAA11751.1; -; Genomic_DNA.
DR EMBL; AL009126; CAB15292.2; -; Genomic_DNA.
DR PIR; D70045; D70045.
DR RefSeq; NP_391182.2; NC_000964.3.
DR RefSeq; WP_003228527.1; NZ_JNCM01000033.1.
DR AlphaFoldDB; O32193; -.
DR SMR; O32193; -.
DR IntAct; O32193; 16.
DR STRING; 224308.BSU33020; -.
DR PaxDb; O32193; -.
DR PRIDE; O32193; -.
DR EnsemblBacteria; CAB15292; CAB15292; BSU_33020.
DR GeneID; 935936; -.
DR KEGG; bsu:BSU33020; -.
DR PATRIC; fig|224308.179.peg.3578; -.
DR eggNOG; COG2205; Bacteria.
DR eggNOG; COG2770; Bacteria.
DR InParanoid; O32193; -.
DR OMA; IKMWMAS; -.
DR PhylomeDB; O32193; -.
DR BioCyc; BSUB:BSU33020-MON; -.
DR BRENDA; 2.7.13.3; 658.
DR Proteomes; UP000001570; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0000155; F:phosphorelay sensor kinase activity; IBA:GO_Central.
DR CDD; cd00082; HisKA; 1.
DR Gene3D; 3.30.565.10; -; 1.
DR InterPro; IPR003660; HAMP_dom.
DR InterPro; IPR003594; HATPase_C.
DR InterPro; IPR036890; HATPase_C_sf.
DR InterPro; IPR005467; His_kinase_dom.
DR InterPro; IPR003661; HisK_dim/P.
DR InterPro; IPR036097; HisK_dim/P_sf.
DR InterPro; IPR004358; Sig_transdc_His_kin-like_C.
DR Pfam; PF00672; HAMP; 1.
DR Pfam; PF02518; HATPase_c; 1.
DR Pfam; PF00512; HisKA; 1.
DR PRINTS; PR00344; BCTRLSENSOR.
DR SMART; SM00304; HAMP; 1.
DR SMART; SM00387; HATPase_c; 1.
DR SMART; SM00388; HisKA; 1.
DR SUPFAM; SSF47384; SSF47384; 1.
DR SUPFAM; SSF55874; SSF55874; 1.
DR PROSITE; PS50885; HAMP; 1.
DR PROSITE; PS50109; HIS_KIN; 1.
PE 1: Evidence at protein level;
KW ATP-binding; Cell membrane; Kinase; Membrane; Nucleotide-binding;
KW Phosphoprotein; Reference proteome; Transferase; Transmembrane;
KW Transmembrane helix; Two-component regulatory system.
FT CHAIN 1..451
FT /note="Sensor histidine kinase CssS"
FT /id="PRO_0000074743"
FT TOPO_DOM 1..9
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 10..30
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 31..165
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 166..186
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 187..451
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT DOMAIN 187..239
FT /note="HAMP"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00102"
FT DOMAIN 247..451
FT /note="Histidine kinase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00107"
FT MOD_RES 250
FT /note="Phosphohistidine; by autocatalysis"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00107"
FT CONFLICT 444
FT /note="T -> S (in Ref. 2; CAA11751)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 451 AA; 52097 MW; DCC269B9038176F0 CRC64;
MKNKPLAFQI WVVISGILLA ISILLLVLFS NTLRDFFTNE TYTTIENEQH VLTEYRLPGS
IERRYYSEEA TAPTTVRSVQ HVLLPENEEA SSDKDLSILS SSFIHKVYKL ADKQEAKKKR
YSADVNGEKV FFVIKKGLSV NGQSAMMLSY ALDSYRDDLA YTLFKQLLFI IAVVILLSWI
PAIWLAKYLS RPLVSFEKHV KRISEQDWDD PVKVDRKDEI GKLGHTIEEM RQKLVQKDET
ERTLLQNISH DLKTPVMVIR GYTQSIKDGI FPKGDLENTV DVIECEALKL EKKIKDLLYL
TKLDYLAKQK VQHDMFSIVE VTEEVIERLK WARKELSWEI DVEEDILMPG DPEQWNKLLE
NILENQIRYA ETKIEISMKQ DDRNIVITIK NDGPHIEDEM LSSLYEPFNK GKKGEFGIGL
SIVKRILTLH KASISIENDK TGVTYRIAVP K
