ID   CSST1_CONGH             Reviewed;          74 AA.
AC   P0DW20;
DT   03-AUG-2022, integrated into UniProtKB/Swiss-Prot.
DT   03-AUG-2022, sequence version 1.
DT   03-AUG-2022, entry version 1.
DE   RecName: Full=Consomatin Gh1 {ECO:0000303|PubMed:35383850};
DE            Short=ConSST Gh1 {ECO:0000305};
DE   AltName: Full=Somatostatin-related peptide {ECO:0000303|PubMed:35383850};
DE            Short=SSRP {ECO:0000303|PubMed:35383850};
DE   Flags: Precursor;
OS   Conus grahami (Cone snail) (Africonus grahami).
OC   Eukaryota; Metazoa; Spiralia; Lophotrochozoa; Mollusca; Gastropoda;
OC   Caenogastropoda; Neogastropoda; Conoidea; Conidae; Conus; Lautoconus.
OX   NCBI_TaxID=308501;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], PROBABLE D-AMINO ACID AT TRP-67, PROBABLE
RP   HYDROXYLATION AT PRO-71, PROBABLE HYDROXYLATION AT PRO-72, PROBABLE
RP   HYDROXYLATION AT PRO-74, AND PROBABLE DISULFIDE BOND.
RC   TISSUE=Venom duct;
RX   PubMed=35319982; DOI=10.1126/sciadv.abk1410;
RA   Ramiro I.B.L., Bjoern-Yoshimoto W.E., Imperial J.S., Gajewiak J.,
RA   Salcedo P.F., Watkins M., Taylor D., Resager W., Ueberheide B.,
RA   Braeuner-Osborne H., Whitby F.G., Hill C.P., Martin L.F., Patwardhan A.,
RA   Concepcion G.P., Olivera B.M., Safavi-Hemami H.;
RT   "Somatostatin venom analogs evolved by fish-hunting cone snails: from prey
RT   capture behavior to identifying drug leads.";
RL   Sci. Adv. 8:eabk1410-eabk1410(2022).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Venom duct;
RX   PubMed=35383850; DOI=10.1093/molbev/msac075;
RA   Koch T.L., Ramiro I.B.L., Florez-Salcedo P., Engholm E., Jensen K.J.,
RA   Chase K., Olivera B.M., Bjoern-Yoshimoto W.E., Safavi-Hemami H.;
RT   "Reconstructing the origins of the somatostatin and allatostatin-C
RT   signaling systems using the accelerated evolution of biodiverse cone snail
RT   venoms.";
RL   Mol. Biol. Evol. 0:0-0(2022).
CC   -!- FUNCTION: Moderately activates human somatostatin receptors (SSTR) with
CC       a preferential activation of SSTR1 and SSTR4. In vivo, does not cause
CC       behavioral changes in mice within a few minutes of intracranial
CC       injection, but causes a progressive loss of movement thereafter. Four
CC       to five hours after injection, mice recover, even with the highest dose
CC       tested. Shows antinociception and antihyperalgesia activities in two
CC       mouse models of acute pain, most probably by acting outside the central
CC       nervous system. {ECO:0000250|UniProtKB:P0DQT5}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305|PubMed:35319982}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom duct.
CC       {ECO:0000305|PubMed:35319982}.
CC   -!- DOMAIN: The cysteine framework is C-C. {ECO:0000305}.
CC   -!- MISCELLANEOUS: This peptide is an evolutionarily optimized stable
CC       analog of somatostatin. In addition, it adopts nearly identical
CC       conformations as in the somatostatin drug analog Octreotide. As this
CC       drug, it contains a D-Trp at the same position, whose synthesis is a
CC       common strategy used for enhancing the metabolic stability of compounds
CC       in drug design. {ECO:0000250|UniProtKB:P0DQT5}.
CC   -!- MISCELLANEOUS: Consomatins evolved by gene duplication of a
CC       'Somatostatin and related peptides (SSRP)' gene expressed in the snail
CC       neuroendocrine system. {ECO:0000269|PubMed:35383850}.
CC   -!- MISCELLANEOUS: Does not activate any of the other 313 GPCRs tested.
CC       Shows little or no activating activity at the SSTR2, SSTR3 and SSTR5.
CC       {ECO:0000250|UniProtKB:P0DQT5}.
CC   -!- SIMILARITY: Belongs to the conotoxin C superfamily. Consomatin family.
CC       {ECO:0000305}.
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PE   1: Evidence at protein level;
KW   D-amino acid; Disulfide bond; G-protein coupled receptor impairing toxin;
KW   Hydroxylation; Secreted; Signal; Toxin.
FT   SIGNAL          1..22
FT                   /evidence="ECO:0000255"
FT   PROPEP          23..57
FT                   /evidence="ECO:0000305|PubMed:35319982"
FT                   /id="PRO_0000456121"
FT   PEPTIDE         58..74
FT                   /note="Consomatin Gh1"
FT                   /evidence="ECO:0000305|PubMed:35319982"
FT                   /id="PRO_0000456122"
FT   MOD_RES         67
FT                   /note="D-tryptophan"
FT                   /evidence="ECO:0000250|UniProtKB:P0DQT5,
FT                   ECO:0000305|PubMed:35319982"
FT   MOD_RES         71
FT                   /note="4-hydroxyproline"
FT                   /evidence="ECO:0000305|PubMed:35319982"
FT   MOD_RES         72
FT                   /note="4-hydroxyproline"
FT                   /evidence="ECO:0000305|PubMed:35319982"
FT   MOD_RES         74
FT                   /note="4-hydroxyproline"
FT                   /evidence="ECO:0000305|PubMed:35319982"
FT   DISULFID        65..70
FT                   /evidence="ECO:0000250|UniProtKB:P0DQT5,
FT                   ECO:0000305|PubMed:35319982"
SQ   SEQUENCE   74 AA;  8383 MW;  8FE0EA683B06ACCE CRC64;
     MQTACWVMVM MMVWITAPLS EGGKLNDVIR GLVPDDVTPQ LILRSLFFHR PSDSVVRPTV
     PVRICYWKVC PPSP