CST12_ARATH
ID CST12_ARATH Reviewed; 63 AA.
AC Q6NN03; Q9LZA2;
DT 23-FEB-2022, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 111.
DE RecName: Full=Protein CYSTEINE-RICH TRANSMEMBRANE MODULE 12 {ECO:0000303|PubMed:29272523};
DE Short=AthCYSTM12 {ECO:0000303|PubMed:29272523};
GN Name=CYSTM12 {ECO:0000303|PubMed:29272523};
GN OrderedLocusNames=At5g04080 {ECO:0000312|Araport:AT5G04080};
GN ORFNames=F8F6_290 {ECO:0000312|EMBL:CAB85528.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] (ISOFORM 2).
RC STRAIN=cv. Columbia;
RX PubMed=11130714; DOI=10.1038/35048507;
RA Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E.,
RA Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M.,
RA Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.,
RA Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M.,
RA Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L.,
RA O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D.,
RA Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M.,
RA Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L.,
RA Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B.,
RA Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P.,
RA Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M.,
RA Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D.,
RA Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A.,
RA Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I.,
RA Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T.,
RA Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A.,
RA McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U.,
RA Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W.,
RA Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M.,
RA Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S.,
RA Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G.,
RA Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W.,
RA Bevan M., Fransz P.F.;
RT "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.";
RL Nature 408:823-826(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Shinn P., Chen H., Cheuk R.F., Kim C.J., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP FUNCTION, HOMODIMERIZATION, INTERACTION WITH CYSTM4; CYSTM6 AND CYSTM7,
RP TISSUE SPECIFICITY, INDUCTION BY SALT, AND SUBCELLULAR LOCATION.
RC STRAIN=cv. Columbia;
RX PubMed=29272523; DOI=10.1093/pcp/pcx202;
RA Xu Y., Yu Z., Zhang D., Huang J., Wu C., Yang G., Yan K., Zhang S.,
RA Zheng C.;
RT "CYSTM, a novel non-secreted cysteine-rich peptide family, involved in
RT environmental stresses in Arabidopsis thaliana.";
RL Plant Cell Physiol. 59:423-438(2018).
CC -!- FUNCTION: Involved in resistance to abiotic stress.
CC {ECO:0000303|PubMed:29272523}.
CC -!- SUBUNIT: Homodimer and heterodimers (PubMed:29272523). Binds weakly to
CC CYSTM4, CYSTM6 and CYSTM7 (PubMed:29272523).
CC {ECO:0000269|PubMed:29272523}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:29272523};
CC Single-pass membrane protein {ECO:0000255}. Cytoplasm
CC {ECO:0000269|PubMed:29272523}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q6NN03-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q6NN03-2; Sequence=VSP_061396, VSP_061397;
CC -!- TISSUE SPECIFICITY: Mostly expressed in roots, flowers and siliques
CC and, to a lower extent, in stems and leaves.
CC {ECO:0000269|PubMed:29272523}.
CC -!- INDUCTION: Induced by salt in shoots. {ECO:0000269|PubMed:29272523}.
CC -!- SIMILARITY: Belongs to the CYSTM1 family. {ECO:0000305}.
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DR EMBL; AL162873; CAB85528.1; -; Genomic_DNA.
DR EMBL; CP002688; AED90694.1; -; Genomic_DNA.
DR EMBL; CP002688; AED90695.1; -; Genomic_DNA.
DR EMBL; BT010917; AAR24695.1; -; mRNA.
DR EMBL; BT011498; AAS00338.1; -; mRNA.
DR PIR; T48435; T48435.
DR RefSeq; NP_196028.2; NM_120490.4.
DR AlphaFoldDB; Q6NN03; -.
DR PaxDb; Q6NN03; -.
DR PRIDE; Q6NN03; -.
DR EnsemblPlants; AT5G04080.1; AT5G04080.1; AT5G04080. [Q6NN03-1]
DR EnsemblPlants; AT5G04080.2; AT5G04080.2; AT5G04080. [Q6NN03-2]
DR GeneID; 830287; -.
DR Gramene; AT5G04080.1; AT5G04080.1; AT5G04080. [Q6NN03-1]
DR Gramene; AT5G04080.2; AT5G04080.2; AT5G04080. [Q6NN03-2]
DR Araport; AT5G04080; -.
DR TAIR; locus:2150685; AT5G04080.
DR eggNOG; ENOG502SDV1; Eukaryota.
DR HOGENOM; CLU_128451_6_1_1; -.
DR InParanoid; Q6NN03; -.
DR OMA; MENNGNR; -.
DR OrthoDB; 1562510at2759; -.
DR PhylomeDB; Q6NN03; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q6NN03; baseline and differential.
DR GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IDA:UniProtKB.
DR GO; GO:0042802; F:identical protein binding; IDA:UniProtKB.
DR GO; GO:0042803; F:protein homodimerization activity; IDA:UniProtKB.
DR InterPro; IPR028144; CYSTM_dom.
DR InterPro; IPR044850; WIH2-like.
DR PANTHER; PTHR31568; PTHR31568; 1.
DR Pfam; PF12734; CYSTM; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cell membrane; Cytoplasm; Membrane;
KW Reference proteome; Transmembrane; Transmembrane helix.
FT CHAIN 1..63
FT /note="Protein CYSTEINE-RICH TRANSMEMBRANE MODULE 12"
FT /id="PRO_0000454809"
FT TRANSMEM 40..56
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 1..34
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 49
FT /note="C -> W (in isoform 2)"
FT /id="VSP_061396"
FT VAR_SEQ 50..63
FT /note="Missing (in isoform 2)"
FT /id="VSP_061397"
SQ SEQUENCE 63 AA; 7322 MW; D4D7A1486AB24CF3 CRC64;
MQDMRDQNPP QGYPAAEQVS EQPGQDKKKK KPRFFETKQK GDRGFIEGCL FALCCCWICE
MCF
