CST12_MOUSE
ID CST12_MOUSE Reviewed; 128 AA.
AC Q9DAN8;
DT 01-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 124.
DE RecName: Full=Cystatin-12;
DE AltName: Full=Cystatin TE-1;
DE Flags: Precursor;
GN Name=Cst12;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND TISSUE SPECIFICITY.
RC STRAIN=C57BL/6J; TISSUE=Testis;
RX PubMed=12444065; DOI=10.1095/biolreprod.102.003368;
RA Li Y., Friel P.J., Robinson M.O., McLean D.J., Griswold M.D.;
RT "Identification and characterization of testis- and epididymis-specific
RT genes: cystatin SC and cystatin TE-1.";
RL Biol. Reprod. 67:1872-1880(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Testis;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: May play a specialized role in spermatogenesis.
CC {ECO:0000269|PubMed:12444065}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Located at the very proximal caput epididymis (at
CC protein level). Expressed in epididymis, Sertoli cells and testis. Also
CC found to be weakly expressed in ovary and prostate.
CC {ECO:0000269|PubMed:12444065}.
CC -!- SIMILARITY: Belongs to the cystatin family. {ECO:0000305}.
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DR EMBL; AF440737; AAL30843.1; -; mRNA.
DR EMBL; AK005670; BAB24179.1; -; mRNA.
DR EMBL; AL844519; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC119432; AAI19433.1; -; mRNA.
DR CCDS; CCDS50737.2; -.
DR RefSeq; NP_081330.1; NM_027054.1.
DR AlphaFoldDB; Q9DAN8; -.
DR SMR; Q9DAN8; -.
DR STRING; 10090.ENSMUSP00000126008; -.
DR MEROPS; I25.024; -.
DR GlyGen; Q9DAN8; 1 site.
DR iPTMnet; Q9DAN8; -.
DR PhosphoSitePlus; Q9DAN8; -.
DR PaxDb; Q9DAN8; -.
DR PRIDE; Q9DAN8; -.
DR ProteomicsDB; 284054; -.
DR DNASU; 69362; -.
DR Ensembl; ENSMUST00000028932; ENSMUSP00000028932; ENSMUSG00000027443.
DR GeneID; 69362; -.
DR KEGG; mmu:69362; -.
DR CTD; 69362; -.
DR MGI; MGI:1916612; Cst12.
DR VEuPathDB; HostDB:ENSMUSG00000027443; -.
DR eggNOG; ENOG502TDK9; Eukaryota.
DR GeneTree; ENSGT00940000162636; -.
DR HOGENOM; CLU_118168_3_2_1; -.
DR InParanoid; Q9DAN8; -.
DR OrthoDB; 1565344at2759; -.
DR PhylomeDB; Q9DAN8; -.
DR BioGRID-ORCS; 69362; 1 hit in 72 CRISPR screens.
DR ChiTaRS; Cst12; mouse.
DR PRO; PR:Q9DAN8; -.
DR Proteomes; UP000000589; Chromosome 2.
DR RNAct; Q9DAN8; protein.
DR Bgee; ENSMUSG00000027443; Expressed in seminiferous tubule of testis and 29 other tissues.
DR ExpressionAtlas; Q9DAN8; baseline and differential.
DR Genevisible; Q9DAN8; MM.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0004869; F:cysteine-type endopeptidase inhibitor activity; IEA:UniProtKB-KW.
DR CDD; cd00042; CY; 1.
DR InterPro; IPR000010; Cystatin_dom.
DR InterPro; IPR046350; Cystatin_sf.
DR Pfam; PF00031; Cystatin; 1.
DR SUPFAM; SSF54403; SSF54403; 1.
PE 1: Evidence at protein level;
KW Disulfide bond; Glycoprotein; Protease inhibitor; Reference proteome;
KW Secreted; Signal; Thiol protease inhibitor.
FT SIGNAL 1..21
FT /evidence="ECO:0000255"
FT CHAIN 22..128
FT /note="Cystatin-12"
FT /id="PRO_0000285791"
FT CARBOHYD 122
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 82..92
FT /evidence="ECO:0000250"
FT DISULFID 105..125
FT /evidence="ECO:0000250"
SQ SEQUENCE 128 AA; 15036 MW; 40AFD00103E55ED6 CRC64;
MLWKSVLSVA LIVLGIHDCS FKFLEIDKNE EEFAISVEHV VFHFNENQDD DFAYKFLRVR
RSLRQKYTLK YLVDLEMGRT LCGKYDEDID NCPLQEGPGE RKVRCTYIVE TEAWVTKFTI
LNSTCVQT
