CST13_MOUSE
ID CST13_MOUSE Reviewed; 141 AA.
AC Q80ZN5; Q9DAP1;
DT 21-JUN-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2003, sequence version 1.
DT 03-AUG-2022, entry version 126.
DE RecName: Full=Cystatin-13;
DE AltName: Full=Cystatin-T;
DE Flags: Precursor;
GN Name=Cst13 {ECO:0000312|MGI:MGI:1916544};
GN Synonyms=Cymg1 {ECO:0000312|EMBL:AAT11922.2};
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1] {ECO:0000305, ECO:0000312|EMBL:AAT11922.2}
RP NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND
RP DEVELOPMENTAL STAGE.
RC TISSUE=Testis {ECO:0000312|EMBL:AAT11922.2};
RX PubMed=15645076; DOI=10.1093/abbs/37.1.11;
RA Xiang Y., Nie D.-S., Wang J., Tan X.-J., Deng Y., Luo S.-W., Lu G.-X.;
RT "Cloning, characterization and primary function study of a novel gene,
RT Cymg1, related to family 2 cystatins.";
RL Acta Biochim. Biophys. Sin. 37:11-18(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Testis;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3] {ECO:0000312|EMBL:AAH48681.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Testis {ECO:0000312|EMBL:AAH48681.1};
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: May perform a specialized role during sperm development and
CC maturation. {ECO:0000303|PubMed:15645076}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}. Cytoplasm
CC {ECO:0000269|PubMed:15645076}. Note=According to PubMed:15645076 it is
CC cytoplasmic.
CC -!- TISSUE SPECIFICITY: Expressed exclusively in testis. Found in
CC spermatagonia, spermatocytes, round spermatids, elongating spermatids
CC and spermatozoa. {ECO:0000269|PubMed:15645076}.
CC -!- DEVELOPMENTAL STAGE: Expressed only in the adult. Levels are low 1 week
CC postpartum, steadily increase 2 to 5 weeks postpartum, are highest at 7
CC weeks and then drop to back the levels found at 5 weeks.
CC {ECO:0000269|PubMed:15645076}.
CC -!- SIMILARITY: Belongs to the cystatin family. {ECO:0000255}.
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DR EMBL; AY600990; AAT11922.2; -; mRNA.
DR EMBL; AK005665; BAB24175.1; -; mRNA.
DR EMBL; BC048681; AAH48681.1; -; mRNA.
DR CCDS; CCDS16849.1; -.
DR RefSeq; NP_081300.1; NM_027024.3.
DR RefSeq; XP_011238068.1; XM_011239766.1.
DR AlphaFoldDB; Q80ZN5; -.
DR SMR; Q80ZN5; -.
DR STRING; 10090.ENSMUSP00000036005; -.
DR MEROPS; I25.027; -.
DR PaxDb; Q80ZN5; -.
DR PRIDE; Q80ZN5; -.
DR ProteomicsDB; 283967; -.
DR DNASU; 69294; -.
DR Ensembl; ENSMUST00000046589; ENSMUSP00000036005; ENSMUSG00000036924.
DR GeneID; 69294; -.
DR KEGG; mmu:69294; -.
DR UCSC; uc008mtq.1; mouse.
DR CTD; 69294; -.
DR MGI; MGI:1916544; Cst13.
DR VEuPathDB; HostDB:ENSMUSG00000036924; -.
DR eggNOG; ENOG502T6MU; Eukaryota.
DR GeneTree; ENSGT00940000163249; -.
DR HOGENOM; CLU_118168_2_2_1; -.
DR InParanoid; Q80ZN5; -.
DR OMA; ETYVYVQ; -.
DR OrthoDB; 1565344at2759; -.
DR PhylomeDB; Q80ZN5; -.
DR BioGRID-ORCS; 69294; 0 hits in 73 CRISPR screens.
DR PRO; PR:Q80ZN5; -.
DR Proteomes; UP000000589; Chromosome 2.
DR RNAct; Q80ZN5; protein.
DR Bgee; ENSMUSG00000036924; Expressed in seminiferous tubule of testis and 9 other tissues.
DR Genevisible; Q80ZN5; MM.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0004869; F:cysteine-type endopeptidase inhibitor activity; IEA:UniProtKB-KW.
DR CDD; cd00042; CY; 1.
DR InterPro; IPR000010; Cystatin_dom.
DR InterPro; IPR046350; Cystatin_sf.
DR Pfam; PF00031; Cystatin; 1.
DR SMART; SM00043; CY; 1.
DR SUPFAM; SSF54403; SSF54403; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Disulfide bond; Protease inhibitor; Reference proteome;
KW Secreted; Signal; Thiol protease inhibitor.
FT SIGNAL 1..24
FT /evidence="ECO:0000255"
FT CHAIN 25..141
FT /note="Cystatin-13"
FT /evidence="ECO:0000255"
FT /id="PRO_0000006661"
FT REGION 76..80
FT /note="Secondary area of contact"
FT /evidence="ECO:0000250, ECO:0000305"
FT SITE 26
FT /note="Reactive site"
FT /evidence="ECO:0000250, ECO:0000305"
FT DISULFID 94..104
FT /evidence="ECO:0000250|UniProtKB:P01034"
FT DISULFID 118..138
FT /evidence="ECO:0000250|UniProtKB:P01034"
FT CONFLICT 97
FT /note="I -> V (in Ref. 2; BAB24175)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 141 AA; 16825 MW; C20FA0D8A884951F CRC64;
MARFLQTLLF LVIMVEFVSR RVEAWGSPQI VRPFEDIPKS YVYVQHALWY AMKEYNKASN
DLYNFRVVDI LKSQEQITDS LEYYLEVNIA RTMCKKIAGD NENCLFQQDP KMKKMVFCIF
IVSSKPWKFE LKMLKKQCKD I
