CST8_MOUSE
ID CST8_MOUSE Reviewed; 142 AA.
AC P32766; O89102; Q543A7;
DT 01-OCT-1993, integrated into UniProtKB/Swiss-Prot.
DT 30-MAY-2000, sequence version 2.
DT 03-AUG-2022, entry version 153.
DE RecName: Full=Cystatin-8;
DE AltName: Full=Cystatin-related epididymal spermatogenic protein;
DE AltName: Full=Cystatin-related epididymal-specific protein;
DE Flags: Precursor;
GN Name=Cst8; Synonyms=Cres;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
RC STRAIN=C3H/HeJ, and CD-1;
RX PubMed=10229662; DOI=10.1042/bj3400085;
RA Cornwall G.A., Hsia N., Sutton H.G.;
RT "Structure, alternative splicing and chromosomal localization of the
RT cystatin-related epididymal spermatogenic gene.";
RL Biochem. J. 340:85-93(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Testis;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Testis;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 4-142.
RC TISSUE=Epididymis;
RX PubMed=1280328; DOI=10.1210/mend.6.10.1280328;
RA Cornwall G.A., Orgebin-Crist M.-C., Hann S.R.;
RT "The CRES gene: a unique testis-regulated gene related to the cystatin
RT family is highly restricted in its expression to the proximal region of the
RT mouse epididymis.";
RL Mol. Endocrinol. 6:1653-1664(1992).
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Performs a specialized role during sperm development and
CC maturation.
CC -!- SUBCELLULAR LOCATION: Secreted.
CC -!- TISSUE SPECIFICITY: Proximal caput region of the epididymis. Lower
CC expression in the testis. Within the testis it is localized to the
CC elongating spermatids, whereas within the epididymis it is exclusively
CC synthesized by the proximal caput epithelium.
CC -!- INDUCTION: Testicular factors or hormones other than androgens present
CC in the testicular fluid may be involved in the regulation of CRES gene
CC expression.
CC -!- SIMILARITY: Belongs to the cystatin family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AF091503; AAC61754.1; -; Genomic_DNA.
DR EMBL; AK018943; BAB31489.1; -; mRNA.
DR EMBL; AK054409; BAC35768.1; -; mRNA.
DR EMBL; AK161808; BAE36585.1; -; mRNA.
DR EMBL; BC049753; AAH49753.1; -; mRNA.
DR EMBL; AF090691; AAC36316.1; -; mRNA.
DR EMBL; S49926; AAC35390.1; -; mRNA.
DR CCDS; CCDS16848.1; -.
DR PIR; A45361; A45361.
DR RefSeq; NP_034108.1; NM_009978.2.
DR RefSeq; XP_006498721.1; XM_006498658.3.
DR RefSeq; XP_006498722.1; XM_006498659.3.
DR PDB; 6UIO; X-ray; 1.83 A; A/B/C/D=31-142.
DR PDBsum; 6UIO; -.
DR AlphaFoldDB; P32766; -.
DR SMR; P32766; -.
DR STRING; 10090.ENSMUSP00000028931; -.
DR MEROPS; I25.027; -.
DR GlyGen; P32766; 2 sites.
DR PhosphoSitePlus; P32766; -.
DR PaxDb; P32766; -.
DR PRIDE; P32766; -.
DR ProteomicsDB; 283968; -.
DR Antibodypedia; 1474; 235 antibodies from 32 providers.
DR DNASU; 13012; -.
DR Ensembl; ENSMUST00000028931; ENSMUSP00000028931; ENSMUSG00000027442.
DR Ensembl; ENSMUST00000109947; ENSMUSP00000105573; ENSMUSG00000027442.
DR GeneID; 13012; -.
DR KEGG; mmu:13012; -.
DR UCSC; uc008mto.1; mouse.
DR CTD; 10047; -.
DR MGI; MGI:107161; Cst8.
DR VEuPathDB; HostDB:ENSMUSG00000027442; -.
DR eggNOG; ENOG502T6MT; Eukaryota.
DR GeneTree; ENSGT00940000162294; -.
DR HOGENOM; CLU_118168_2_2_1; -.
DR InParanoid; P32766; -.
DR OMA; YFIDVEI; -.
DR OrthoDB; 1565344at2759; -.
DR PhylomeDB; P32766; -.
DR BioGRID-ORCS; 13012; 0 hits in 71 CRISPR screens.
DR ChiTaRS; Cst8; mouse.
DR PRO; PR:P32766; -.
DR Proteomes; UP000000589; Chromosome 2.
DR RNAct; P32766; protein.
DR Bgee; ENSMUSG00000027442; Expressed in seminiferous tubule of testis and 33 other tissues.
DR Genevisible; P32766; MM.
DR GO; GO:0009986; C:cell surface; IDA:MGI.
DR GO; GO:0005737; C:cytoplasm; IDA:MGI.
DR GO; GO:0005576; C:extracellular region; IDA:MGI.
DR GO; GO:0004869; F:cysteine-type endopeptidase inhibitor activity; IEA:UniProtKB-KW.
DR CDD; cd00042; CY; 1.
DR InterPro; IPR000010; Cystatin_dom.
DR InterPro; IPR046350; Cystatin_sf.
DR Pfam; PF00031; Cystatin; 1.
DR SMART; SM00043; CY; 1.
DR SUPFAM; SSF54403; SSF54403; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Disulfide bond; Glycoprotein; Protease inhibitor;
KW Reference proteome; Secreted; Signal; Thiol protease inhibitor.
FT SIGNAL 1..19
FT /evidence="ECO:0000255"
FT CHAIN 20..142
FT /note="Cystatin-8"
FT /id="PRO_0000006654"
FT MOTIF 77..81
FT /note="Secondary area of contact"
FT /evidence="ECO:0000255"
FT CARBOHYD 39
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 100
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 95..105
FT /evidence="ECO:0000250"
FT DISULFID 119..139
FT /evidence="ECO:0000250"
FT CONFLICT 4..15
FT /note="PLWLSLILFIIP -> GTRDEQVGESQK (in Ref. 4; AAC35390)"
FT /evidence="ECO:0000305"
FT STRAND 34..37
FT /evidence="ECO:0007829|PDB:6UIO"
FT HELIX 43..59
FT /evidence="ECO:0007829|PDB:6UIO"
FT STRAND 63..100
FT /evidence="ECO:0007829|PDB:6UIO"
FT TURN 111..113
FT /evidence="ECO:0007829|PDB:6UIO"
FT STRAND 116..126
FT /evidence="ECO:0007829|PDB:6UIO"
FT HELIX 127..129
FT /evidence="ECO:0007829|PDB:6UIO"
FT STRAND 131..141
FT /evidence="ECO:0007829|PDB:6UIO"
SQ SEQUENCE 142 AA; 16288 MW; 50B446E98F66673E CRC64;
MAKPLWLSLI LFIIPVALAV GVDQSKNEVK AQNYFGSINI SNANVKQCVW FAMKEYNKES
EDKYVFLVDK ILHAKLQITD RMEYQIDVQI SRSNCKKPLN NTENCIPQKK PELEKKMSCS
FLVGALPWNG EFNLLSKECK DV
