ID   CST8_RAT                Reviewed;         142 AA.
AC   O88969;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1998, sequence version 1.
DT   03-AUG-2022, entry version 122.
DE   RecName: Full=Cystatin-8;
DE   AltName: Full=Cystatin-related epididymal spermatogenic protein;
DE   Flags: Precursor;
GN   Name=Cst8; Synonyms=Cres;
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=Sprague-Dawley; TISSUE=Epididymis;
RX   PubMed=10229662; DOI=10.1042/bj3400085;
RA   Cornwall G.A., Hsia N., Sutton H.G.;
RT   "Structure, alternative splicing and chromosomal localization of the
RT   cystatin-related epididymal spermatogenic gene.";
RL   Biochem. J. 340:85-93(1999).
CC   -!- FUNCTION: Performs a specialized role during sperm development and
CC       maturation.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the cystatin family. {ECO:0000305}.
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DR   EMBL; AF090692; AAC36317.1; -; mRNA.
DR   RefSeq; NP_062131.1; NM_019258.1.
DR   AlphaFoldDB; O88969; -.
DR   SMR; O88969; -.
DR   STRING; 10116.ENSRNOP00000006667; -.
DR   MEROPS; I25.027; -.
DR   GlyGen; O88969; 1 site.
DR   PaxDb; O88969; -.
DR   Ensembl; ENSRNOT00000006667; ENSRNOP00000006667; ENSRNOG00000004989.
DR   GeneID; 29679; -.
DR   KEGG; rno:29679; -.
DR   UCSC; RGD:2434; rat.
DR   CTD; 10047; -.
DR   RGD; 2434; Cst8.
DR   eggNOG; ENOG502T6MT; Eukaryota.
DR   GeneTree; ENSGT00940000162294; -.
DR   HOGENOM; CLU_118168_2_2_1; -.
DR   InParanoid; O88969; -.
DR   OrthoDB; 1565344at2759; -.
DR   PhylomeDB; O88969; -.
DR   PRO; PR:O88969; -.
DR   Proteomes; UP000002494; Chromosome 3.
DR   Genevisible; O88969; RN.
DR   GO; GO:0009986; C:cell surface; ISO:RGD.
DR   GO; GO:0005737; C:cytoplasm; ISO:RGD.
DR   GO; GO:0005576; C:extracellular region; ISO:RGD.
DR   GO; GO:0004869; F:cysteine-type endopeptidase inhibitor activity; IEA:UniProtKB-KW.
DR   CDD; cd00042; CY; 1.
DR   InterPro; IPR000010; Cystatin_dom.
DR   InterPro; IPR046350; Cystatin_sf.
DR   Pfam; PF00031; Cystatin; 1.
DR   SMART; SM00043; CY; 1.
DR   SUPFAM; SSF54403; SSF54403; 1.
PE   2: Evidence at transcript level;
KW   Disulfide bond; Glycoprotein; Protease inhibitor; Reference proteome;
KW   Secreted; Signal; Thiol protease inhibitor.
FT   SIGNAL          1..19
FT                   /evidence="ECO:0000255"
FT   CHAIN           20..142
FT                   /note="Cystatin-8"
FT                   /id="PRO_0000006655"
FT   MOTIF           77..81
FT                   /note="Secondary area of contact"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        100
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   DISULFID        95..105
FT                   /evidence="ECO:0000250"
FT   DISULFID        119..139
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   142 AA;  16246 MW;  FB873FAA6B6CAB34 CRC64;
     MTKPLLLSLI FFIIPAALAV DVDQSKNEVK AQRYFGSISI SNANVKQCVW FAMKEYNKGS
     EDKYLFLLDK TLHATLQITD RMEYHIDVQI SRSNCRKPLN NTENCIPQKN PKLEKKLSCS
     FLVGALPWNG EFDLLSKECK DV