CST9L_HUMAN
ID CST9L_HUMAN Reviewed; 147 AA.
AC Q9H4G1; B2R5A1;
DT 23-JAN-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 164.
DE RecName: Full=Cystatin-9-like;
DE AltName: Full=Testatin {ECO:0000303|PubMed:11756564};
DE Flags: Precursor;
GN Name=CST9L; Synonyms=CTES7B {ECO:0000303|PubMed:20565543};
GN ORFNames=UNQ1835/PRO3543;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=12975309; DOI=10.1101/gr.1293003;
RA Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J.,
RA Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P.,
RA Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E., Heldens S., Huang A.,
RA Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D.,
RA Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L.,
RA Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C.,
RA Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J.,
RA Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M.;
RT "The secreted protein discovery initiative (SPDI), a large-scale effort to
RT identify novel human secreted and transmembrane proteins: a bioinformatics
RT assessment.";
RL Genome Res. 13:2265-2270(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Testis;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=11780052; DOI=10.1038/414865a;
RA Deloukas P., Matthews L.H., Ashurst J.L., Burton J., Gilbert J.G.R.,
RA Jones M., Stavrides G., Almeida J.P., Babbage A.K., Bagguley C.L.,
RA Bailey J., Barlow K.F., Bates K.N., Beard L.M., Beare D.M., Beasley O.P.,
RA Bird C.P., Blakey S.E., Bridgeman A.M., Brown A.J., Buck D., Burrill W.D.,
RA Butler A.P., Carder C., Carter N.P., Chapman J.C., Clamp M., Clark G.,
RA Clark L.N., Clark S.Y., Clee C.M., Clegg S., Cobley V.E., Collier R.E.,
RA Connor R.E., Corby N.R., Coulson A., Coville G.J., Deadman R., Dhami P.D.,
RA Dunn M., Ellington A.G., Frankland J.A., Fraser A., French L., Garner P.,
RA Grafham D.V., Griffiths C., Griffiths M.N.D., Gwilliam R., Hall R.E.,
RA Hammond S., Harley J.L., Heath P.D., Ho S., Holden J.L., Howden P.J.,
RA Huckle E., Hunt A.R., Hunt S.E., Jekosch K., Johnson C.M., Johnson D.,
RA Kay M.P., Kimberley A.M., King A., Knights A., Laird G.K., Lawlor S.,
RA Lehvaeslaiho M.H., Leversha M.A., Lloyd C., Lloyd D.M., Lovell J.D.,
RA Marsh V.L., Martin S.L., McConnachie L.J., McLay K., McMurray A.A.,
RA Milne S.A., Mistry D., Moore M.J.F., Mullikin J.C., Nickerson T.,
RA Oliver K., Parker A., Patel R., Pearce T.A.V., Peck A.I.,
RA Phillimore B.J.C.T., Prathalingam S.R., Plumb R.W., Ramsay H., Rice C.M.,
RA Ross M.T., Scott C.E., Sehra H.K., Shownkeen R., Sims S., Skuce C.D.,
RA Smith M.L., Soderlund C., Steward C.A., Sulston J.E., Swann R.M.,
RA Sycamore N., Taylor R., Tee L., Thomas D.W., Thorpe A., Tracey A.,
RA Tromans A.C., Vaudin M., Wall M., Wallis J.M., Whitehead S.L.,
RA Whittaker P., Willey D.L., Williams L., Williams S.A., Wilming L.,
RA Wray P.W., Hubbard T., Durbin R.M., Bentley D.R., Beck S., Rogers J.;
RT "The DNA sequence and comparative analysis of human chromosome 20.";
RL Nature 414:865-871(2001).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [6]
RP PROTEIN SEQUENCE OF 29-43.
RX PubMed=15340161; DOI=10.1110/ps.04682504;
RA Zhang Z., Henzel W.J.;
RT "Signal peptide prediction based on analysis of experimentally verified
RT cleavage sites.";
RL Protein Sci. 13:2819-2824(2004).
RN [7]
RP TISSUE SPECIFICITY, AND VARIANT PRO-109.
RX PubMed=11756564; DOI=10.1093/molehr/8.1.8;
RA Eriksson A., Toehoenen V., Wedell A., Nordqvist K.;
RT "Isolation of the human testatin gene and analysis in patients with
RT abnormal gonadal development.";
RL Mol. Hum. Reprod. 8:8-15(2002).
RN [8]
RP TISSUE SPECIFICITY.
RX PubMed=20565543; DOI=10.1111/j.1525-142x.2010.00418.x;
RA Frygelius J., Arvestad L., Wedell A., Toehoenen V.;
RT "Evolution and human tissue expression of the Cres/Testatin subgroup genes,
RT a reproductive tissue specific subgroup of the type 2 cystatins.";
RL Evol. Dev. 12:329-342(2010).
CC -!- INTERACTION:
CC Q9H4G1; A8MQ03: CYSRT1; NbExp=3; IntAct=EBI-3923092, EBI-3867333;
CC Q9H4G1; P60411: KRTAP10-9; NbExp=3; IntAct=EBI-3923092, EBI-10172052;
CC Q9H4G1; Q6L8H4: KRTAP5-1; NbExp=3; IntAct=EBI-3923092, EBI-12074540;
CC Q9H4G1; A8MW99: MEI4; NbExp=3; IntAct=EBI-3923092, EBI-19944212;
CC Q9H4G1; Q7Z3S9: NOTCH2NLA; NbExp=3; IntAct=EBI-3923092, EBI-945833;
CC Q9H4G1; P0DPK4: NOTCH2NLC; NbExp=3; IntAct=EBI-3923092, EBI-22310682;
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Specifically expressed in testis.
CC {ECO:0000269|PubMed:11756564, ECO:0000269|PubMed:20565543}.
CC -!- MISCELLANEOUS: Lacks critical consensus sites important for cysteine
CC protease inhibition. {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the cystatin family. {ECO:0000305}.
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DR EMBL; AY358615; AAQ88978.1; -; mRNA.
DR EMBL; AK312112; BAG35048.1; -; mRNA.
DR EMBL; AL121894; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH471133; EAX10140.1; -; Genomic_DNA.
DR EMBL; BC029656; AAH29656.1; -; mRNA.
DR CCDS; CCDS13157.1; -.
DR RefSeq; NP_542177.1; NM_080610.2.
DR AlphaFoldDB; Q9H4G1; -.
DR SMR; Q9H4G1; -.
DR BioGRID; 126159; 84.
DR IntAct; Q9H4G1; 9.
DR STRING; 9606.ENSP00000366178; -.
DR GlyGen; Q9H4G1; 2 sites.
DR iPTMnet; Q9H4G1; -.
DR PhosphoSitePlus; Q9H4G1; -.
DR BioMuta; CST9L; -.
DR DMDM; 20137970; -.
DR MassIVE; Q9H4G1; -.
DR PaxDb; Q9H4G1; -.
DR PeptideAtlas; Q9H4G1; -.
DR PRIDE; Q9H4G1; -.
DR ProteomicsDB; 80835; -.
DR Antibodypedia; 42869; 101 antibodies from 19 providers.
DR DNASU; 128821; -.
DR Ensembl; ENST00000376979.4; ENSP00000366178.3; ENSG00000101435.5.
DR GeneID; 128821; -.
DR KEGG; hsa:128821; -.
DR MANE-Select; ENST00000376979.4; ENSP00000366178.3; NM_080610.3; NP_542177.1.
DR UCSC; uc002wtk.5; human.
DR CTD; 128821; -.
DR DisGeNET; 128821; -.
DR GeneCards; CST9L; -.
DR HGNC; HGNC:16233; CST9L.
DR HPA; ENSG00000101435; Group enriched (epididymis, testis).
DR MIM; 616536; gene.
DR neXtProt; NX_Q9H4G1; -.
DR OpenTargets; ENSG00000101435; -.
DR PharmGKB; PA26982; -.
DR VEuPathDB; HostDB:ENSG00000101435; -.
DR eggNOG; ENOG502TAJ0; Eukaryota.
DR GeneTree; ENSGT00940000161877; -.
DR HOGENOM; CLU_118168_3_1_1; -.
DR InParanoid; Q9H4G1; -.
DR OMA; TISTEPW; -.
DR OrthoDB; 1577727at2759; -.
DR PhylomeDB; Q9H4G1; -.
DR PathwayCommons; Q9H4G1; -.
DR SignaLink; Q9H4G1; -.
DR BioGRID-ORCS; 128821; 13 hits in 1061 CRISPR screens.
DR GeneWiki; CST9L; -.
DR GenomeRNAi; 128821; -.
DR Pharos; Q9H4G1; Tdark.
DR PRO; PR:Q9H4G1; -.
DR Proteomes; UP000005640; Chromosome 20.
DR RNAct; Q9H4G1; protein.
DR Bgee; ENSG00000101435; Expressed in left testis and 51 other tissues.
DR ExpressionAtlas; Q9H4G1; baseline and differential.
DR Genevisible; Q9H4G1; HS.
DR GO; GO:0005615; C:extracellular space; IBA:GO_Central.
DR GO; GO:0004869; F:cysteine-type endopeptidase inhibitor activity; IEA:UniProtKB-KW.
DR GO; GO:0019730; P:antimicrobial humoral response; IBA:GO_Central.
DR CDD; cd00042; CY; 1.
DR InterPro; IPR043250; CST9-like.
DR InterPro; IPR000010; Cystatin_dom.
DR InterPro; IPR046350; Cystatin_sf.
DR PANTHER; PTHR46945; PTHR46945; 1.
DR Pfam; PF00031; Cystatin; 1.
DR SUPFAM; SSF54403; SSF54403; 1.
PE 1: Evidence at protein level;
KW Direct protein sequencing; Disulfide bond; Glycoprotein;
KW Protease inhibitor; Reference proteome; Secreted; Signal;
KW Thiol protease inhibitor.
FT SIGNAL 1..28
FT /evidence="ECO:0000269|PubMed:15340161"
FT CHAIN 29..147
FT /note="Cystatin-9-like"
FT /id="PRO_0000006656"
FT CARBOHYD 117
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 139
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 98..108
FT /evidence="ECO:0000250"
FT DISULFID 122..142
FT /evidence="ECO:0000250"
FT VARIANT 109
FT /note="H -> P (in dbSNP:rs2295564)"
FT /evidence="ECO:0000269|PubMed:11756564"
FT /id="VAR_022079"
SQ SEQUENCE 147 AA; 17276 MW; 3A3286FFADCA4FD6 CRC64;
MLGLPWKGGL SWALLLLLLG SQILLIYAWH FHEQRDCDEH NVMARYLPAT VEFAVHTFNQ
QSKDYYAYRL GHILNSWKEQ VESKTVFSME LLLGRTRCGK FEDDIDNCHF QESTELNNTF
TCFFTISTRP WMTQFSLLNK TCLEGFH
