CSTF1_MOUSE
ID CSTF1_MOUSE Reviewed; 431 AA.
AC Q99LC2; Q3UMB8; Q8BJW1; Q9CSU8;
DT 07-JUN-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 158.
DE RecName: Full=Cleavage stimulation factor subunit 1;
DE AltName: Full=CF-1 50 kDa subunit;
DE AltName: Full=Cleavage stimulation factor 50 kDa subunit;
DE Short=CSTF 50 kDa subunit;
DE Short=CstF-50;
GN Name=Cstf1;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Egg, Embryo, and Mammary gland;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=FVB/N; TISSUE=Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Spleen, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: One of the multiple factors required for polyadenylation and
CC 3'-end cleavage of mammalian pre-mRNAs (By similarity). May be
CC responsible for the interaction of CSTF with other factors to form a
CC stable complex on the pre-mRNA (By similarity).
CC {ECO:0000250|UniProtKB:Q05048}.
CC -!- SUBUNIT: Homodimer (By similarity). The CSTF complex is composed of
CC CSTF1 (50 kDa subunit), CSTF2 (64 kDa subunit) and CSTF3 (77 kDa
CC subunit) (By similarity). Interacts (via repeats WD) directly with
CC CSTF3 (By similarity). Interacts (via repeat WD6) with BARD1 (By
CC similarity). Interacts with ERCC6 (By similarity).
CC {ECO:0000250|UniProtKB:Q05048}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q05048}.
CC -!- DOMAIN: N-terminus mediates homodimerization.
CC {ECO:0000250|UniProtKB:Q05048}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAB27929.1; Type=Frameshift; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AK011943; BAB27929.1; ALT_FRAME; mRNA.
DR EMBL; AK050686; BAC34379.1; -; mRNA.
DR EMBL; AK078667; BAC37353.1; -; mRNA.
DR EMBL; AK145001; BAE26180.1; -; mRNA.
DR EMBL; BC003440; AAH03440.1; -; mRNA.
DR CCDS; CCDS17130.1; -.
DR RefSeq; NP_077161.1; NM_024199.2.
DR RefSeq; XP_006500135.2; XM_006500072.3.
DR AlphaFoldDB; Q99LC2; -.
DR SMR; Q99LC2; -.
DR BioGRID; 212117; 8.
DR STRING; 10090.ENSMUSP00000112076; -.
DR iPTMnet; Q99LC2; -.
DR PhosphoSitePlus; Q99LC2; -.
DR EPD; Q99LC2; -.
DR MaxQB; Q99LC2; -.
DR PaxDb; Q99LC2; -.
DR PeptideAtlas; Q99LC2; -.
DR PRIDE; Q99LC2; -.
DR ProteomicsDB; 277904; -.
DR Antibodypedia; 28869; 243 antibodies from 31 providers.
DR DNASU; 67337; -.
DR Ensembl; ENSMUST00000116375; ENSMUSP00000112076; ENSMUSG00000027498.
DR GeneID; 67337; -.
DR KEGG; mmu:67337; -.
DR UCSC; uc008ocp.1; mouse.
DR CTD; 1477; -.
DR MGI; MGI:1914587; Cstf1.
DR VEuPathDB; HostDB:ENSMUSG00000027498; -.
DR eggNOG; KOG0640; Eukaryota.
DR GeneTree; ENSGT00910000144253; -.
DR HOGENOM; CLU_041619_0_0_1; -.
DR InParanoid; Q99LC2; -.
DR OMA; SNRCINT; -.
DR OrthoDB; 587820at2759; -.
DR PhylomeDB; Q99LC2; -.
DR TreeFam; TF314234; -.
DR Reactome; R-MMU-72163; mRNA Splicing - Major Pathway.
DR Reactome; R-MMU-72187; mRNA 3'-end processing.
DR Reactome; R-MMU-73856; RNA Polymerase II Transcription Termination.
DR Reactome; R-MMU-77595; Processing of Intronless Pre-mRNAs.
DR BioGRID-ORCS; 67337; 25 hits in 77 CRISPR screens.
DR PRO; PR:Q99LC2; -.
DR Proteomes; UP000000589; Chromosome 2.
DR RNAct; Q99LC2; protein.
DR Bgee; ENSMUSG00000027498; Expressed in spermatid and 236 other tissues.
DR ExpressionAtlas; Q99LC2; baseline and differential.
DR Genevisible; Q99LC2; MM.
DR GO; GO:0005848; C:mRNA cleavage stimulating factor complex; IBA:GO_Central.
DR GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR GO; GO:0031124; P:mRNA 3'-end processing; IEA:InterPro.
DR Gene3D; 1.20.960.50; -; 1.
DR Gene3D; 2.130.10.10; -; 2.
DR InterPro; IPR044633; CstF1-like.
DR InterPro; IPR032028; CSTF1_dimer.
DR InterPro; IPR038184; CSTF1_dimer_sf.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_repeat.
DR InterPro; IPR019775; WD40_repeat_CS.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR PANTHER; PTHR44133; PTHR44133; 1.
DR Pfam; PF16699; CSTF1_dimer; 1.
DR Pfam; PF00400; WD40; 5.
DR SMART; SM00320; WD40; 6.
DR SUPFAM; SSF50978; SSF50978; 1.
DR PROSITE; PS00678; WD_REPEATS_1; 1.
DR PROSITE; PS50082; WD_REPEATS_2; 4.
DR PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE 1: Evidence at protein level;
KW mRNA processing; Nucleus; Reference proteome; Repeat; WD repeat.
FT CHAIN 1..431
FT /note="Cleavage stimulation factor subunit 1"
FT /id="PRO_0000050945"
FT REPEAT 106..145
FT /note="WD 1"
FT REPEAT 171..210
FT /note="WD 2"
FT REPEAT 215..254
FT /note="WD 3"
FT REPEAT 260..301
FT /note="WD 4"
FT REPEAT 303..343
FT /note="WD 5"
FT REPEAT 395..431
FT /note="WD 6"
SQ SEQUENCE 431 AA; 48382 MW; 8BD7BEFE422824D2 CRC64;
MYRTKVGLKD RQQLYKLIIS QLLYDGYISI ANGLINEIKP QSVCAPSEQL LHLIKLGMEN
DDTAVQYAIG RSDTVAPGTG IDLEFDADVQ TMSPEASEYE TCYVTSHKGP CRVATYSRDG
QLIATGSADA SIKILDTERM LAKSAMPIEV MMNETAQQNM ENHPVIRTLY DHVDEVTCLA
FHPTEQILAS GSRDYTLKLF DYSKPSAKRA FKYIQEAEML RSISFHPSGD FILVGTQHPT
LRLYDINTFQ CFVSCNPQDQ HTDAICSVNY NPSANMYVTG SKDGCIKLWD GVSNRCITTF
EKAHDGAEVC SAIFSKNSKY ILSSGKDSVA KLWEISTGRT LVRYTGAGLS GRQVHRTQAV
FNHTEDYILL PDERTISLCC WDSRTAERRN LLSLGHNNIV RCIVHSPTNP GFMTCSDDFR
ARFWYRRSTT D
